China
Africa
Explore chapters and articles related to this topic
Interleukin-8
Published in Jason Kelley, Cytokines of the Lung, 2022
Robert M. Strieter, Theodore J. Standiford, Mark W. Rolfe, Steven L. Kunkel
Confusion in this area has been greatly clarified by the isolation, purification, cloning, and expression of the potent PMN chemotactic and activating factor IL-8 (Yoshimura et al., 1987a,b; Schroeder and Christophers, 1986; Schroeder et al., 1987; Walz et al., 1987; Peveri et al., 1988; Van Damme et al., 1988; Schmid and Weissmann, 1987; Matsushima et al., 1988; Lindley et al., 1988). Several groups have isolated this polypeptide from peripheral blood monocytes, endothelial cells, or fibroblasts and have termed this factor neutrophil-activating peptide (NAP-1: Schroeder and Christophers, 1988; Schroeder et al., 1987; Larsen et al., 1989; Baggiolini et al., 1989), neutrophil chemotactic factor (NCF: Strieter et al., 1988, 1989a,b), monocyte-derived neutrophil chemotactic factor (MDNCF: Yoshimura et al., 1987a,b; Matsushima et al., 1988), neutrophil-activating factor (NAF: Walz et al., 1987; Peveri et al., 1988), and granulocyte chemotactic factor (GCF: Van Damme et al., 1988). The term interleukin-8 (IL-8) was proposed and endorsed at a meeting of investigators studying this cytokine (Westwick et al., 1989).
Immune Modulation In Sepsis
Published in Thomas F. Kresina, Immune Modulating Agents, 2020
Janet M. J. Hammond, Peter D. Potgieter
Interleukin 8 Interleukin 8 (IL-8) is a proinflammatory cytokine that serves as a potent chemoattractant factor and activator of neutrophils; it is produced by many cell types after stimulation by IL-1, TNF, or microbial products such as endotoxin. Its release causes neutrophil degranulation, and priming by TNF seems to enhance IL-8-promoted degranulation [78]. It has been detected in the urine of patients with urinary tract infections [79], and in the cerebrospinal fluid of patients with meningococcal disease [80].
Whole-body vibration training
Published in Claudio F. Donner, Nicolino Ambrosino, Roger S. Goldstein, Pulmonary Rehabilitation, 2020
A RCT by Greulich et al. (24) investigated the use of WBVT in COPD patients during hospitalization due to an acute exacerbation of COPD. While receiving similar medical treatment, 40 COPD patients (FEV1: 36 ± 16% pred.) were randomly assigned to one of two different interventions. Both groups received 30 min of daily chest physiotherapy focusing on breathing exercises to improve mucus clearance. A WBVT group also exercised on a side-alternating vibration platform (Galileo, Novotec Medical, Germany) at a high frequency (26 Hz) for 3 × 2 min daily. Although patients stayed in hospital for only 1 week, authors of this study reported very large and remarkable benefits for patients in the WBVT group. Patients in the control group showed worsened exercise capacity and quality of life at discharge, whereas patients in the WBVT group significantly improved in these outcomes (6MWD: −5 m vs. +96 m, p < 0.01; COPD Assessment Test: control group: 24 ± 9 pts to 23 ± 7; p = not significant, and WBVT-group: 29 ± 6 to 25 ± 6; p = 0.02). Another important finding of this trial was that the decrease in interleukin-8, an inflammatory marker, was significantly more pronounced in the WBVT group (p = 0.04).
Inhibitory effect of recombinant human CXCL8(3-72)K11R/G31P on atherosclerotic plaques in a mouse model of atherosclerosis
Published in Immunopharmacology and Immunotoxicology, 2019
Yuanhua Qin, Weifeng Mao, Lingmin Pan, Yunliang Sun, Fushun Fan, Ying Zhao, Ying Cui, Xiaoqing Wei, Kazuhiro Kohama, Fang Li, Ying Gao
Atherosclerosis is a pathological process that will induce cardiovascular disease. Recent studies demonstrated that inflammation plays an important role in coronary heart disease, and inflammatory factors are involved in atherosclerosis development [1–8]. Interleukin-8 (IL-8) is an important inflammatory factor and is associated with the development of atherosclerosis [9–12]. High levels of IL-8 are associated with an increased risk of coronary artery disease [13–15]. CXCR1 and CXCR2 are receptors of IL-8, in which CXCR1 also binds to CXCL6/7 but CXCR2 displays higher affinity to IL-8 [16,17]. CXCR2 is a therapeutic target to reduce inflammation [18,19]. CXCL8(3-72)K11R (G31P) is a human IL-8 analog, in which the 11th amino acid (AA) lysine was substituted to arginine, and the 31st AA glycine was substituted to proline [20]. G31P analog has higher affinity to receptors and binds to both CXCR1 and CXCR2 of neutrophils to function in regulating inflammation [21,22]. 0.5 nM G31P can suppress the chemotaxis of neutrophils similar with the levels of 129 nM IL-8/CXCL8 [23]. Some studies reported the roles of CXCL8/G31P on the modulation of inflammation-related angiogenesis and ulcerative colitis [24,25]. However, the role of G31P in atherosclerosis is not defined.
Uptake and molecular impact of aluminum-containing nanomaterials on human intestinal caco-2 cells
Published in Nanotoxicology, 2018
Holger Sieg, Caroline Braeuning, Birgitta Maria Kunz, Hannes Daher, Claudia Kästner, Benjamin-Christoph Krause, Thomas Meyer, Pégah Jalili, Kevin Hogeveen, Linda Böhmert, Dajana Lichtenstein, Agnès Burel, Soizic Chevance, Harald Jungnickel, Jutta Tentschert, Peter Laux, Albert Braeuning, Fabienne Gauffre, Valérie Fessard, Jan Meijer, Irina Estrela-Lopis, Andreas F. Thünemann, Andreas Luch, Alfonso Lampen
The levels of interleukin-8 (IL-8) in cell media were measured using an enzyme-linked immunosorbent assay (ELISA). Primary IL-8 antibody (M801), biotin-conjugated human IL-8 (M802B), recombinant IL-8 cytokine, HRP-Conjugated Streptavidin (N100), SuperBlock blocking buffer, 3,3′,5,5′-tetramethylbenzidine (TMB), tumor necrosis factor alpha (TNF-α) and Tween 20 were obtained from Thermofisher scientific. 20 ng/mL tumor necrosis factor alpha (TNF-α) was used as positive control. Following 24-h incubation with particles, media were collected and frozen at 20 °C until analysis. 96 well microplates (Nunc) were coated with human recombinant IL-8 primary antibodies at 1 µg/mL and incubated overnight at 4 °C. Between each step, wells were washed with PBS-Tween 20 (0.05%). After saturation with SuperBlock for 1 h, samples and standards were added into the wells and incubated at room temperature for 2 h. Biotin-conjugated human IL-8 antibodies (0.1 µg/ml) were then added for 1 h followed by 100 µL of HRP 1:10,000 labeling for 45 min. Finally, 100 µL of the chromogenic substrate TMB was added and the reaction was stopped with 100 µL of H2SO4 (1 M). Plates were read at 405 nm. The concentrations of IL-8 expressed in fold of negative control were calculated against a standard curve prepared in duplicate.
Effect of Eicosapentaenoic Acid on Body Composition and Inflammation Markers in Patients with Head and Neck Squamous Cell Cancer from a Public Hospital in Mexico
Published in Nutrition and Cancer, 2018
Obed Solís-Martínez, Valentina Plasa-Carvalho, Geraldine Phillips-Sixtos, Yanelly Trujillo-Cabrera, Arturo Hernández-Cuellar, Gloria E. Queipo-García, Eduardo Meaney-Mendiolea, Guillermo M. Ceballos-Reyes, Vanessa Fuchs-Tarlovsky
The results obtained from the assessment of pro-inflammatory cytokine levels are shown in Table 2. There was a significant difference between interleukin-8 levels (p = 0.020) and tendencies toward a decrease of TNF-α, IL-1β, IL-6, and IFN-γ levels in the experimental group (p < 0.05). Comparing the results to the base line, both groups had a decrease in C-reactive protein (CRP) at the end of the clinical trial, but was statistically significant in the control group (25 ± 38 g/dl versus 12 ± 18 g/dl, p = 0.048); however, there were no statistical differences between experimental and control group (p > 0.05) at the end of the study.