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Definition of HLA-Dw Determinants Using Homozygous Typing Cells and the Mixed Lymphocyte Culture
Published in M. Kam, Jeffrey L. Bidwell, Handbook of HLA TYPING TECHNIQUES, 2020
Care should be taken when handling blood, serum, or cells because of the possibility of Hepatitis B and human immunodeficiency virus (HIV) infection. Blood obtained by venepuncture is collected in sterile bottles containing 10 U/ml preservative-free heparin and is diluted with an equal volume of RPMI 1640 or similar medium. 10 ml of the diluted blood is layered onto 4 ml of Ficoll-Hypaque in 15 ml tubes and centrifuged at 800 × g for 25 min.
Translating the Medical Record
Published in Walter F. Stanaszek, Mary J. Stanaszek, Robert J. Holt, Steven Strauss, Understanding Medical Terms, 2020
Walter F. Stanaszek, Mary J. Stanaszek, Robert J. Holt, Steven Strauss
Blood studies assess disorders of cell production (hematopoiesis), synthesis, and function. Examination of the blood and bone marrow are the primary means of determining blood disorders. Venipuncture is used to procure larger samples of blood for testing. Bone marrow specimens are obtained through needle biopsy or aspiration. A hemogram includes platelet count, white blood cell count (WBC), red blood cell count (RBC), hematocrit (Hct), and indices. A complete blood count (CBC) includes a hemogram plus differential count.
Biochemical Parameters and Childhood Nutritional Anemia
Published in Anil Gupta, Biochemical Parameters and the Nutritional Status of Children, 2020
The method requires venipuncture to collect a blood sample. Additionally, technical staff, a laboratory setup, and medical personnel are required to perform the method. Therefore, its use in villages and remote locations is difficult.
A simple method of axillary venipuncture using single landmark for pacemaker leads implantation
Published in Acta Cardiologica, 2023
Peng Zhao, Ying Wang, Shan Zeng
We are proposing a simple and operational method of axillary venipuncture with a high success rate. Considering that subclavian vein is located beneath the medial third of clavicle and anatomical adjacent relationship between axillary vein and the connecting point of medial and middle third of clavicle is relatively fixed, our approach of axillary venipuncture uses this point as the sole landmark. Deflecting lateral 45° and inserting the needle 30–45° angle relative to skin can ensure that extra-thoracic portion of the vessel is punctured and minimise the risk of pneumothorax associated with a deep puncture that passes through intercostal space. Clavicle anatomy is unaffected by body type, which makes locating process simple and time-saving. Furthermore, the most common techniques describing axillary venipuncture require a skin incision or pacemaker pocket made prior to vein puncture [7,12]. Once the puncture fails, additional surgical wounds have been caused. Conversely, our approach can avoid unnecessary surgical cutting by making skin incision after successful puncture.
Literature review on the efficacy of near-infrared device in improving peripheral venous access time and number of attempts in pediatric patients
Published in Current Medical Research and Opinion, 2023
Salem Khalaf Al Anazi, Alexander Woodman, Waleed Abdullah Al Zahrani, Mohammed Abdulaziz Alsanad, Matar Saeed Alzahrani, Faisal Ramadan Alanazi, Mohammad Rasheed
Venipuncture is a routine procedure in healthcare settings. The prevalence of venipuncture is up to 90% and 99.6% in young children receiving an IV treatment in the neonatal PICU27,31–33. Further evidence suggests that the procedure can be especially difficult and painful in infants and children due to smaller vessel diameters, difficulty in palpation of veins, and visibility in newborns. As a result, healthcare providers often fail to access peripheral veins with only one attempt. Many patients require 2–11 attempts to access the vein27,31–33. Failed venipuncture can cause vein thrombosis, hematoma, or even nerve damage involving the lateral anterior brachial cutaneous nerve (LACN), which can lead to so-called “causalgia” or complex regional pain syndrome (CRPS)33–36. Therefore, the NIR devices have become a new revelatory tool in the healthcare industry that is expected to provide faster vein access and be less time-consuming34–36.
An 8-year Analysis of Magnesium Status in Elite International Track & Field Athletes
Published in Journal of the American College of Nutrition, 2020
N. Pollock, R. Chakraverty, I. Taylor, S. C. Killer
Blood screening for RCMg status was conducted as part of general performance screening all athletes between 2 and 4 times per year. These were always performed during athlete training phases between October and August, when athletes were undertaking regular running and strength and conditioning sessions. Blood screening was not taken during rest phases of the training cycle. Athletes were asked to avoid high intensity strength and running training or competition for at least 12 h prior to the blood test but no other restrictions were placed on training. Subjects were not asked to fast prior to the test. Venepuncture was performed with a 21- or 23-gauge butterfly needle with the athlete seated or prone lying at 45 degrees and collected into a vacutainer (BD Vacutainer® lithium heparin blood collection tubes). All blood samples were analyzed by The Doctor’s Laboratory (TDL Ltd, London) using RCMg analysis. The analysis performed was inductively coupled plasma mass spectrometry (ICP-MS) with a known laboratory coefficient of variation of 2.5%. Demographic information including age, gender and athletic event was recorded.