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Nanopharmaceuticals in Alveolar Bone and Periodontal Regeneration
Published in Harishkumar Madhyastha, Durgesh Nandini Chauhan, Nanopharmaceuticals in Regenerative Medicine, 2022
Mark A. Reynolds, Zeqing Zhao, Michael D. Weir, Tao Ma, Jin Liu, Hockin H. K. Xu, Abraham Schneider
The root surfaces of healthy human teeth following extraction provide a significant source of hPDLSCs. The PDL tissues contain various cell types including fibroblasts, cementoblasts, osteoblasts, endothelial cells, and epithelial cells (Lekic et al. 2010). Regeneration of the periodontal attachment apparatus, including new bone, cementum, and periodontal ligament, involves highly coordinated osteogenesis, cementogenesis, and fibrogenesis because a new fibrous attachment is dependent on formation of new bone and cementum (Zeichner-David 2006).
Concavities of Crystalline Sintered Hydroxyapatite-Based Macroporous Bioreactors Initiate the Spontaneous Induction of Bone Formation
Published in Ugo Ripamonti, The Geometric Induction of Bone Formation, 2020
Further contradictions with misplaced quotations are evident under Discussion where it is reported that “BMPs (e.g. BMP-2 and BMP-7) were detected in cells involved in bone morphogenesis initiated by osteoinductive CaP ceramics [40,41]”. Reference [40], as quoted by Zhang et al. (2014), refers to the induction of BMPs’ gene expression during endochondral bone formation as initiated by naturally derived porcine transforming growth factor-β1 (pTGF-β1) delivered by allogeneic insoluble collagenous bone matrix and not by CaP constructs (Duneas et al. 1998). Reference [41], as listed by Zhang et al. (2014), reports the induction of cementogenesis by recombinant human osteogenic protein-1 (hOP-1) when delivered by insoluble collagenous matrices and not CaP ceramics to furcation defects of the Chacma baboon Papio ursinus (Ripamonti et al. 1996).
Biomimetic Approaches for the Design and Development of Multifunctional Bioresorbable Layered Scaffolds for Dental Regeneration
Published in Vincenzo Guarino, Marco Antonio Alvarez-Pérez, Current Advances in Oral and Craniofacial Tissue Engineering, 2020
Campodoni Elisabetta, Dozio Samuele Maria, Mulazzi Manuela, Montanari Margherita, Montesi Monica, Panseri Silvia, Sprio Simone, Tampieri Anna, Sandri Monica
The events constituting tooth formation are complex, they require the active presence of all cellular components of the periodontium: fibroblasts for soft connective tissues such as PDL, cementoblasts for cementogenesis, osteoblasts for bone and endothelial cells for angiogenesis (Bartold et al. 2000; Maeda et al. 2014). All these cell lineages must correctly interact with each other, as well as with a variety of molecules of the extracellular matrix (ECM) (Thesleff and Hurmerinta 1981).
CKIP-1 mediates P. gingivalis-suppressed osteogenic/cementogenic differentiation of periodontal ligament cells partially via p38 signaling pathway
Published in Journal of Oral Microbiology, 2023
Xin Huang, Junhong Xiao, Huiyi Wang, Yan Peng, Heyu Liu, Li Ma, Xiaoxuan Wang, Zhengguo Cao
500 ng RNA extracted with TRIzol (TaKaRa) was reverse transcribed to cDNA by PrimeScript RT Reagent (TaKaRa) following the manufacturer’s protocol. qPCR was conducted with SYBR qPCR Master Mix (Vazyme) and specific primers (Sangon) by Applied Biosystems 7500. Thermocycling conditions are shown below: 95°C for 30 s; 40 cycles at 95°C for 10 s, 62°C for 34 s; 72°C for 30 s. Values were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and quantified by 2ΔΔCt method. Primers for OCN, BSP, cementum attachment protein (CAP), cementum protein-1 (CEMP-1), IL-6, IL-8, IL-1β, MMP-1, MMP-3, CKIP-1 and GADPH are shown in Table S1. OCN and BSP were used to indicate both osteogenesis and cementogenesis, while CAP and CEMP-1 were used to indicate cementogenesis only.
Focusing on Hippo Pathway in Stem Cells of Oral Origin, Enamel Formation and Periodontium Regeneration
Published in Organogenesis, 2022
Tianyi Wang, Kehan Li, Hanghang Liu, En Luo
Cementoblasts are cells forming the cementum, which provides attachment of Sharpey’s fiber of the periodontal ligament.46 OE YAP displayed upregulated mRNA levels of cementogenesis phenotypic markers like ALP, RUNX2, dentin matrix acidic phosphoprotein 1 (DMP1) and OCN, while knockdown of YAP showed lower levels of these markers, suggesting that YAP promoted the differentiation and mineralization of cementoblasts.8