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Extremophilic Microbes and their Extremozymes for Industry and Allied Sectors
Published in Ajar Nath Yadav, Ali Asghar Rastegari, Neelam Yadav, Microbiomes of Extreme Environments, 2021
Hiran Kanti Santra, Debdulal Banerjee
Violacein is a water insoluble purple color pigment having antibacterial and antioxidative properties, produced by Chromobacterium violaceum that grows specifically on a medium containing tryptophan (Soliev et al. 2011). This bacterium is a common habitat of soil and water. Prodigiosin (red pyrrole-containing pigment) is produced by actinobacterial and gamma-proteobacterial members like Streptomyces coelicolor, Hahella chejuensis, Pseudomonas magnesiorubra, Vibrio psychroerythreus, and Serratia sp. (Williamson et al. 2006). Some strains of psychrophilic Janthinobacterium lividum are found to produce red colored prodigiosin and purple colored violacein. Purple and red colored strains exhibited antibiosis in the terms of high level of resistance against β-lactam antibiotics (Schloss et al. 2010). Prodigiosin are of clinical importance as they act as immunosuppressive and anticancer agents (Williamson et al. 2006).
Production, characterization, and powder preparation of quorum quenching acylase AiiO for pathogen control
Published in Preparative Biochemistry and Biotechnology, 2019
Yue Li, Jing Zhao, Chunshan Quan, Liming Jin, Yongbin Xu, Ming Chen
AHL-degrading activity of AHL acylase AiiO was at first determined with the bioassay indicator strain C. violaceum CV026. LB agar containing 2% agar was spread with 1 mL of C. violaceum CV026 overnight culture and divided into two parts with a sterile knife. Each part of LB agar was covered with a filter membrane. Purified AiiO protein with the final concentration of 30 mg/L and C6-HSL of 40 µM were resolved in 100 mM Tris buffer (pH 7.0) and reacted at 30 °C for 4 h. The reaction mixture of 10 μL (marked with E) and an equal volume of C6-HSL solution of 40 µM (marked with C) was spotted separately in each filter membrane on the LB agar, which was incubated at 30 °C for 24 h to observe color changes. The indicator strain C. violaceum CV026 responds to C6-HSL with violacein pigment production.[15]