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Interfacial Catalysis at Oil/Water Interfaces
Published in Alexander G. Vdlkdv, Interfacial Catalysis, 2002
Dependence of the catalytic activity (maximal rate) of (a) glyceraldehyde-3-phosphate dehydrogenase, GAPDH; (b) lactate dehydrogenase, LDH; and (c) their heteroenzyme complexes, GAPDH + LDH, on the degree of surfactant (water-to-AOT molar ratio) in reverse micelles of AOT in octane. (From Ref. 60. )
Biological Process for Ethanol Production
Published in Jay J. Cheng, Biomass to Renewable Energy Processes, 2017
In the presence of NAD+ (Nicotinamide adenine dinucleotide) and phosphorus, glyceraldehyde 3-phosphate is catalyzed by glyceraldehyde 3-phosphate dehydrogenase to release a hydrogen to NAD+ and take the phosphorus to form 1,3-diphosphoglycerate.
Cytocompatibility and osteogenic differentiation of stem cells from human exfoliated deciduous teeth with cotton cellulose nanofibers for tissue engineering and regenerative medicine
Published in Journal of Biomaterials Science, Polymer Edition, 2022
Rafaella de S. S. Zanette, Leonara Fayer, Eduarda R. de Oliveira, Camila G. Almeida, Cauê R. Oliveira, Luiz F. C. de Oliveira, Carlos M. C. Maranduba, Érika C. Alvarenga, Humberto M. Brandão, Michele Munk
The cells were treated with various cotton CNF concentrations (0, 0.1, 1, 10, 50, and 100 µg mL−1) for 48 h, and total RNA was extracted from three pools of 2 × 105 cells per group using the RNeasy Micro Kit instructions (Qiagen, Hilden, Germany) and treated with DNase. According to the manufacturer’s instructions, the cDNA was generated from total RNA by reverse transcription using SuperScript III First-Strand Synthesis Supermix (Invitrogen, Carlsbad, CA, USA). Quantification of RNA and cDNA from each pooled sample was performed using a 1 µL sample in a spectrophotometer (Nanodrop, Wilmington, DE, USA). Relative quantification was performed in six-plicate using real-time PCR (Applied Biosystems 7500 Fast Real-Time PCR System, Foster City, CA, USA). Reactions were prepared using a mixture of SYBR Green PCR Master Mix (Applied Biosystems), primers, nuclease-free water, and cDNA. For glyceraldehyde-3-phosphate dehydrogenase (GAPDH), adenine phosphoribosyltransferase (APTR), Death Associated Protein Kinase 1 (DAPK1) genes, 150 ng cDNA per reaction were used, whereas for genes Heat Shock Protein Family A member 5 (HSPA5), Activating Transcription Factor 4 (ATF4), B-cell lymphoma 2 (BCL2) and BCL2 associated X protein (BAX) 600 ng cDNA were used per reaction.
Phytotoxic effect of silver nanoparticles on seed germination and growth of terrestrial plants
Published in Journal of Environmental Science and Health, Part C, 2019
Shruti Budhani, Nzube Prisca Egboluche, Zikri Arslan, Hongtao Yu, Hua Deng
Besides proteins related to defense mechanism (like oxidative stress), proteins involved in photosynthesis appears to have interested many investigators. Jhanzab et al.52 carried out proteomic analysis in wheat (T. aestivum L.) where growth was promoted by AgNP blended with nicotinic acid and KNO3. This was achieved by the regulation of energy metabolism due to suppression of glycolysis. Increased proteins included those associated with photosynthesis and glycolysis (such as glyceraldehyde-3-phosphate dehydrogenase). Antioxidant enzyme activities such as SOD, catalase, and peroxidase are promoted. Suppressed proteins were those related to glycolysis, signaling, cell wall, redox and mitochondrial electron transport chain as well as phosphoenol pyruvate carboxylase.
Effects of glycerol and glucose on docosahexaenoic acid synthesis in Aurantiochyrium limacinum SFD-1502 by transcriptome analysis
Published in Preparative Biochemistry & Biotechnology, 2023
Huaqiu Zhang, Xiangying Zhao, Chen Zhao, Jiaxiang Zhang, Yang Liu, Mingjing Yao, Jianjun Liu
Pyruvate is a precursor of acetyl-CoA and an end product of EMP pathway. Compared with glycerol, the pyruvate kinase (PK) using glucose as carbon source, which is the rate-limiting enzyme in the glycolytic pathway, was upregulated during the fermentation, especially was upregulated by 3-fold at 24 hr. Moreover, when cultured with glucose, the expression of fructose diphosphate aldolase (FBAld), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 3-phosphoglycerate kinase (3PGK) which were also involved in glycolysis was obviously higher than cultured with glycerol. Therefore, it can be concluded that the EMP pathway was more active in the group glucose than in group glycerol.