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Chemical Permeabilization of Cells for Intracellular Product Release
Published in Juan A. Asenjo, Separation Processes in Biotechnology, 2020
Thomas J. Naglak, David J. Hettwer, Henry Y. Wang
Plant cells have been permeabilized for product release by the use of organic solvents, most notably DMSO. Brodelius and Nilsson (1983) report the use of immobilized Catharanthus roseus cells permeabilized with DMSO. Treatment with 5% DMSO releases 85 to 90% of intracellular ajmalicine, a circulatory drug, within a few minutes. The immobilized cells remain viable after this permeabilization, allowing for cyclic operation in three phases: cell growth, ajmalicine production, and ajmalicine release by DMSO permeabilization. Different concentrations of DMSO can be used to permeabilize other species of plant cells, such as Daucus carota and Datura innoxia (Brodelius and Nilsson, 1983). Other solvents, lysolecithin, antibiotics, and basic proteins have also been employed for permeabilization of plant cells (Felix et al., 1981). In the case of lysolecithin, the permeability of soybean cells, for example, can be controlled by varying the concentration of lysolecithin and the duration of exposure (Cannon et al., 1987).
Antibacterial and anticancer activity (PANC-1) of green synthesized copper oxide nanoparticles from Catharanthus roseus
Published in Inorganic and Nano-Metal Chemistry, 2023
S. Karthika, P. Kanchana, B. Prabha Devi, S. Shanmuga Sundari
This work primarily focuses on green synthesis method for synthesis of copper oxide from Catharanthus roseus. Researchers reported that Catharanthus roseus (C. roseus) (Apocynaceae) is one of the important medicinal plants, due to the presence of the indispensable anti-cancer drugs, i.e., vincristine and vinblastine. Roots of this plant are the main source of the anti-hypertension alkaloid ajmalicine. It contains more than 130 mono-terpenoid indole alkaloids.[25] It has medicinal importance owing to the presence of alkaloids like ajamalicine, serpentine and reserpine, which are well known for their hypotensive and antispasmodic properties.[26] Improving glucose uptake in pancreatic or muscle cells could amend the hyperglycemic conditions of type 2 diabetes.