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Chitosan: A Versatile Biomaterial for the 21st Century
Published in Shakeel Ahmed, Aisverya Soundararajan, Marine Polysaccharides, 2018
A. Shajahan, V. Kaviyarasan, V. Narayanan, S. Ignacimuthu
A membrane composed of an alginate layer and a chitosan layer with sustained antimicrobial efficacy was prepared. In this study, ciprofloxacin HCl was incorporated into the alginate layer. The membrane was found to control bacterial growth persistently. The results suggested that this chitosan/alginate composite membrane incorporated with ciprofloxacin HCl has the potential for wound-dressing application [246]. Basic fibroblast growth factor (bFGF) has been shown to stimulate wound healing. However, consistent delivery of bFGF has been problematic. Mizuno et al. [247] studied the stability of bFGF incorporated into a chitosan film as a delivery vehicle for providing sustained release of bFGF. The therapeutic effect of this system on wound healing in genetically diabetic mice was determined as a model for treating clinically impaired wound healing. A chitosan film was prepared by freeze-drying hydroxypropyl chitosan in acetate buffer solution. Results suggested that chitosan itself facilitates wound repair and that bFGF incorporated into a chitosan film is a stable delivery vehicle for accelerating wound healing. HemCon®, ChitoFlex® PRO and ChitoGauze® PRO bandages are engineered chitosan-based preparations designed as haemostatic dressing and as topical antimicrobial dressing. The conflicting clamping and stimulating effects of chitosan acetate bandage on normal wounds were studied by removing the bandage from wounds at times after application ranging from 1 h to 9 days. The results showed that the 3-day application gave the earliest wound closure, and all application times gave a faster healing slope after removal compared with control wounds. Chitosan acetate bandage reduced the number of inflammatory cells in the wound at days 2 and 4 and had an overall beneficial effect on wound healing, especially during the early period, where its antimicrobial effect was most important [248].
Highly efficient soluble expression and purification of recombinant human basic fibroblast growth factor (hbFGF) by fusion with a new collagen-like protein (Scl2) in Escherichia coli
Published in Preparative Biochemistry & Biotechnology, 2020
Inamur Rahman, Lina Fang, Zhang Wei, Xiaodong Zheng, Lian Jiazhang, Lei Huang, Zhinan Xu
Fibroblast growth factor-2 (FGF-2), a.k.a. basic fibroblast growth factor (bFGF), is one of the important members of the fibroblast growth factors (FGFs) family comprising of 24 members of monomeric proteins with similar biological functions. bFGF is a multifunctional and most abundant FGFs family member found mainly in the brain, kidney, and pituitary gland,[1,2] and involved in various pathophysiological processes such as proliferation, angiogenesis, differentiation, and embryogenesis.[3,4] In addition, FGFs are proposed to play a significant role in the development and functions of many organ systems, such as cardiovascular system, nervous system, reproductive system, lungs, and hematopoietic system.[5,6] Because of its high therapeutic values, large-scale production of human bFGF (hbFGF) with high yield is demanded.
Dramatic promotion of wound healing using a recombinant human-like collagen and bFGF cross-linked hydrogel by transglutaminase
Published in Journal of Biomaterials Science, Polymer Edition, 2019
Yayuan Guo, Bing Xu, Yihang Wang, Yan Li, He Si, Xiaoyan Zheng, Zhuoyue Chen, Fulin Chen, Daidi Fan
It has been found that many growth factors, play a significant role in the dynamic stages [2, 3], such as vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF) and epidermal growth factors (EGF). The bFGF, which is the primary promoter for cell proliferation [4, 5], has been shown to play a part in some fields of the medicine, including neovascularization [6], wound repair [7] and tissue regeneration [8], as well as reconstruction of cartilage and bone [9]. In addition, the released bFGF has obvious chemotactic activity on wound cells, and is able to induce some types of cells, including inflammatory cells, fibroblasts, vascular endothelial cells to get together at the wound site [10], activating the phagocytic function of macrophages and clearing local necrotic tissue and bacteria to improve the body’s immune activity, which significantly reduce the chance of wound tissue infection [11]. However, the bFGF could lose its bioactivity rapidly in normal physiological conditions without stabilization [12]. Therefore, it is essential to integrate the bFGF into sustainable drug-releasing systems, such as hydrogels, which can enhance the efficiency of bFGF utilization.
A bilayer scaffold prepared from collagen and carboxymethyl cellulose for skin tissue engineering applications
Published in Journal of Biomaterials Science, Polymer Edition, 2018
Cemile Kilic Bektas, Ilgin Kimiz, Aylin Sendemir, Vasif Hasirci, Nesrin Hasirci
Basic fibroblastic growth factor (bFGF) is a heparin binding growth factor and is an important angiogenic factor that maintains the integrity and development of the vessels during embryogenesis, wound healing and plays a role in tumor vascularization [48]. It was also shown that bFGF expression significantly increases particularly when dermal fibroblasts and keratinocytes are co-cultured in a skin tissue engineering model, and draws attention to the importance of paracrine signaling between the two cell types and effects of this signaling on skin regeneration [55]. Present results indicate that bFGF expression of the cells seeded on BLColl scaffolds increased continuously and reached 72 pg/mL on Day 7 (Figure 7). However, bFGF expression on BLCollCS scaffolds presented a peak around 62 pg/mL on day 4 and then decreased. It is seen in the SEM micrographs that keratinocytes form dense layers on the surface of CollCS scaffolds faster than on Coll scaffolds. Since bFGF is primarily related to proliferation and motility [56], it is possible that the expression of bFGF by keratinocytes decreases after the keratinocyte proliferation is completed. MTT data on mono cultures also show that there is no increase in keratinocyte numbers on CollCS scaffolds after Day 3.