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Two-Dimensional Nanomaterials for Drug Delivery in Regenerative Medicine
Published in Harishkumar Madhyastha, Durgesh Nandini Chauhan, Nanopharmaceuticals in Regenerative Medicine, 2022
Zahra Mohammadpour, Seyed Morteza Naghib
Graphene-based nanomaterials have shown excellent prospects in immunotherapy. Gurunathan et al. conducted a mechanistic study on the pathways involved in cellular toxicity and immunomodulatory of GO and vanillin-functionalised GO (V-rGO) (Gurunathan et al. 2019). Molecular evaluations were performed on THP-1 cells, a human acute monocytic leukaemia cell line. The results revealed that both nanomaterials stimulated cancer cell lines to release cytokines and chemokines, including IL1-β, TNF-α, GM-CSF, IL-6, IL-8, and MCP-1. However, the released cytokines were significantly higher in the case of V-rGO than that observed for GO. GO has been employed as a carrier for the delivery of immunostimulatory CpG oligodeoxynucleotides (ODNs) (Zhang et al. 2017a; Tao et al. 2014). Zhang et al. showed that the cellular uptake of CpG ODNs that were loaded on GO-chitosan (GO-CS) nanocomposites was higher than free CpG ODNs and GO/CpG ODNs complexes (Zhang et al. 2017a). Higher levels of interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) confirmed the observation. Tao et al. further showed that CpG delivery was controlled by the photothermal effect of GO-PEG-PEI (Tao et al. 2014).
Design, Synthesis, and Studies of Novel Piperidine Substituted Triazine Derivatives as Potential Anti-Inflammatory and Antimicrobial Agents
Published in Cristobal N. Aguilar, Suresh C. Ameta, A. K. Haghi, Green Chemistry and Biodiversity, 2019
Pro-inflammatory cytokine production by lip polysaccharide (LPS) in THP-1 cells was measured according to the method described by Hwang et al.27 During the assay, THP-1 cells were cultured in RPMI 1640 culture medium (Gibco BRL, Pasley, UK) containing 100 U/mL Penicillin and 100 mg/mL Streptomycin containing 10% fetal bovine serum (FBS, JRH). Cells were differentiated with phorbol myristate acetate (PMA, Sigma). Following cell plating, the test compounds 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-N-phenylpiperazine-1-carbox-amide urea derivatives (3a-h) and (4-(4,6-dimethoxy-1,3,5-triazin-2-yl)piperazin-1-yl)(phenyl)methanone amide derivatives (3i-p) in 0.5% DMSO were added to each well separately and the plate was incubated for 30 min at 37°C. Finally, LPS (E. coli 0127:B8, Sigma Chemical Co., St. Louis, MO) was added, at a final concentration of 1 μg/mL in each well. Plates were further incubated at 37°C for 24 h in 5% CO2. After incubation, supernatants were harvested, and assayed for TNF-α and IL-6 by ELISA as described by the manufacturer (BD Biosciences). The % inhibitions are measured at 10 μM concentration. The Dexamethasone is used as standard drug. The results are tabulated in Tables 15.7 and 15.10.
Nanoparticles as Nitroso-Glutathion Vehicles
Published in Bertrand Henri Rihn, Biomedical Application of Nanoparticles, 2017
Luc Ferrari, Roudayna Diab, Christophe Nemos, Ramia Safar, Chloe Puisney
We reanalyzed the data of Safar et al. (2016) following criteria selected for our study. Indeed, Safar et al. exposed the same nanoparticles as ours to a monocytes-macrophages model: THP-1. We then compared the differentially regulated gene groups following treatments. It appears that the response of THP-1 involved more genes than Caco-2 response. This difference could be explained by the fact that THP-1 cells are a model of monocytes–macrophages and thus represent the immune system of the body. Whereas Caco-2 cells represents the digestive system of the body less reactive than the immune system. Also, the signaling pathway mainly significantly affected by our conditions was the cytokine–cytokine receptor pathway in THP-1, but this route was not significantly affected on the Caco-2 model. This supports a specific cell response. While working with titanium dioxide nanoparticles, Tilton et al. also demonstrated that THP-1 response differed from Caco-2 response model (Tilton et al. 2015). This suggests that study of the adaptive response following exposure to drug delivery nanoparticles should be performed on different cell models to predict various potentially harmful effects of these compounds.
Comparison of biological responses between submerged, pseudo-air-liquid interface, and air-liquid interface exposure of A549 and differentiated THP-1 co-cultures to combustion-derived particles
Published in Journal of Environmental Science and Health, Part A, 2022
Kamaljeet Kaur, Raziye Mohammadpour, Anne Sturrock, Hamidreza Ghandehari, Christopher Reilly, Robert Paine, Kerry E. Kelly
THP-1 were differentiated into pulmonary macrophage-like cells using phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich, St. Louis, MO).[29] THP-1 cells with a concentration of 860 K-1000K cells/mL were suspended in the media with 0.5 µg/mL PMA, and 9 mL of the suspension was seeded in T25K flask. After 90 min, as described by Kasurinen et al.,[30] the floating cells were aspirated, and 7 mL of phosphate buffer solution (PBS; PBS tablet, VWR Life Science, PA, USA) was added into the flask. The flask was gently tapped to resuspend the cells, transferred to a 50 mL vial, followed by centrifugation at 1.2 K xg for 6 min. The cells were washed twice with PBS before resuspending in DMEM (with FBS). These differentiated THP-1 cells were subsequently seeded onto A549 cells in a co-culture, as discussed in the next paragraph.
Effect of collection methods on combustion particle physicochemical properties and their biological response in a human macrophage-like cell line
Published in Journal of Environmental Science and Health, Part A, 2019
Kamaljeet Kaur, Isabel C. Jaramillo, Raziye Mohammadpour, Anne Sturrock, Hamidreza Ghandehari, Christopher Reilly, Robert Paine, Kerry E. Kelly
THP-1 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in complete growth medium (Roswell Park Memorial Institute medium 1640 with L-glutamine and 0.05 mM 2-mercaptoethanol (Bio-Rad, Inc. Hercules, CA) and 10% Fetal Bovine Serum (GE Healthcare Bio-Sciences, Inc. Marlborough, MA)). THP-1 cells are a pro-monocytic cell line that are differentiated to pulmonary macrophage-like cells in 200 nM of phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich, St. Louis, MO), as described by Daigneault et al.[36] and O’Reilly et al.[37] Cell-cultures were incubated at 37 °C in 5% CO2 and 95% humidified air and kept in the logarithmic phase of growth throughout all experiments.
Pro-inflammatory responses induced by A. fumigatus and A. versicolor in various human macrophage models
Published in Journal of Toxicology and Environmental Health, Part A, 2019
Elisabeth Øya, Anita Solhaug, Anette K. Bølling, Reidun Øvstebø, Tonje B. Steensen, Anani K.J. Afanou, Jørn A. Holme
The human leukemia monocytic cell line THP-1 is widely used to study monocyte/macrophage function (Chanput, Mes, and Wichers 2014; Lund et al. 2016). Treatment with phorbol-12-myristate-13-acetate (PMA) stimulates differentiation into adherent macrophage-like cells (THP-1 Ma) with a pro-inflammatory phenotype (Daigneault et al. 2010; Park et al. 2007; Starr et al. 2018). In vivo and ex vivo macrophage differentiations from human blood monocytes are driven by human granulocyte-macrophage colony–stimulating factor (GM-CSF), which also results in a pro-inflammatory phenotype of MDM (Riddy et al. 2018). The ex vivo/in vitro model of cultured sputum macrophages/airway macrophages (AM) is a new interesting model suggested to represent normal tissue-specific macrophages derived from the surface of the central airways (Bølling et al. 2018).