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Adipose Tissue-Derived Adult Stem Cells
Published in Richard K. Burt, Alberto M. Marmont, Stem Cell Therapy for Autoimmune Disease, 2019
Laura Aust, Lyndon Cooper, Blythe Devlin, Tracey du Laney, Sandra Foster, Jeffrey M. Gimble, Farshid Guilak, Yuan Di C. Halvorsen, Kevin Hicok, Amy Kloster, Henry E. Rice, Anindita Sen, Robert W. Storms, William O. Wilkison
After expansion in culture, the primary isolated human ADAS cells display a distinct phenotype based on cell surface protein expression (characterized by flow cytometric analysis) and cytokine expression (characterized by polymerase chain reaction and ELISA) (Table 2). The profile is similar, but not identical, to that described for human bone marrow stromal cells.10 For example, unlike human bone marrow stromal cells, ADAS cells do not express the STRO-1 (a marker of osteoprogenitor stem cells) surface antigen or the interleukin 1 cytokines.10,21 The ADAS surface protein phenotype is also similar to that described for skeletal muscle derived adult stem cells. 14a,b Both populations express the common acute lymphoblastic leukemia antigen (CD10), aminopeptidase (CD13), and the major histocompatibility antigen class I; however, unlike muscle derived adult stem cells, ADAS cells do not express the neural cell adhesion molecule or N-CAM (CD56).14a,22
Gene Therapy in Tissue Engineering: Prospects and Challenges
Published in Rajesh K. Kesharwani, Raj K. Keservani, Anil K. Sharma, Tissue Engineering, 2022
Bone marrow-derived mesenchymal cells are the adult stem cells for bone and cartilage repair and are derived from the generic marrow of the osteogenic lineage (Bruder et al., 1998; Yang et al., 2001; Howard et al., 2002). These can be isolated via antibody selection procedure by targeting cell surface receptor on the marrow tissues such as endoglin (Haynesworth et al., 1992; Majumdar et al., 2000) and Stro-1 (Simmons and Torok-Storb, 1991; Howard et al., 2002; Stewart et al., 2003). Stromal vascular fraction (SVF) is the source for adipose-derived stem cells (ADSCs) harvested during liposuction. A processed lipoaspirate filtrate generates ADSCs after subsequent digestion, washing, and filtration in the presence of fetal bovine serum (Zuk et al., 2001). Analogous to mesenchymal stem cells, ADSCs exhibit tremendous potential to differentiate into all mesenchymal lineages and possess MSC-like immunophenotypes (Boquest et al., 2005). They are also known to support allogenic transplantations by immunosuppression of mixed lymphocyte reaction and lymphocyte proliferation (Puissant et al., 2005). These can also differentiate into smooth muscle cells as well as endothelial cells by treating with TGF-β1 and bone morphogenetic protein 4 (BMP4), leading to synthesis of collagen and vessel walls, thus is an important source for vascular regeneration (Chang et al., 2010; Zang et al., 2011). Recently, research is more focused toward the applicability quotient since the discovery that umbilical cord-derived mesenchymal stem cells (UC-MSCs) can undergo multilineage differentiation. Umbilical cord cells (UB-MSCs) are very similar to mesenchymal stem cells, as they can differentiate into many cell types such as adipocytes, osteoblasts (Lee et al., 2004), hepatocytes (Kang et al., 2006), and neuronal-like cells (Hou et al., 2003).
Mesenchymal Stem Cells from Dental Tissues
Published in Vincenzo Guarino, Marco Antonio Alvarez-Pérez, Current Advances in Oral and Craniofacial Tissue Engineering, 2020
Febe Carolina Vázquez Vázquez, Jael Adrián Vergara-Lope Núñez, Juan José Montesinos, Patricia González-Alva
Tooth Germ Progenitor Cells (TGPCs) are a stem cell population identified in the dental mesenchyme of the third molar tooth germ during the late bell stage. TGPCs can be expanded in plastic culture plates and maintained for approximately 60 populations doublings, during which they retain their spindle-shaped morphology and high proliferation rate. TGPCs express the MSCs associated markers STRO-1 and CD73, CD90, CD105 and CD166, but are negative for CD34, CD45 and CD133 (Yalvac et al. 2010).
Poly-ε-caprolactone/poly(rotaxane) seeded with human dental pulp stem cells or osteoblasts promotes angiogenesis: a chorioallantoic membrane assay
Published in Journal of Biomaterials Science, Polymer Edition, 2022
Natacha Kalline de Oliveira, Emanuela Prado Ferraz, Flávia Cristina Perillo Rosin, Luciana Correa, Maria Cristina Zindel Deboni
The stemness properties of the hDPSCs were confirmed using flow cytometry (FACSCalibur, BD Biosciences, USA) with a panel of primary antibodies including CD14-PE, CD44-APC, CD45-APC (BD Biosciences, Franklin Lakes, New Jersey, USA), CD146-APC (BioLegend, San Diego, CA, USA), CD34-FITC (Dako, Santa Clara, CA, USA), STRO-1-FITC (BD Biosciences), and the unbound factory antibody, Nanog, and its isotypic controls, IgG2k-FITC (BD Biosciences).