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Some Points of Grammar and Style
Published in Edward J. Rothwell, Michael J. Cloud, Engineering Writing by Design, 2017
Edward J. Rothwell, Michael J. Cloud
5.5. Fix each run-on sentence. (a) We treated an analogous system in Chapter 1 the method of solution is the same.(b) We refer to expressions of this type as waves additional waves are treated later in this section.(c) To evaluate x, multiply (1) through by y and integrate this gives x = 2/c.(d) Equation (2) is the f-transformation it is closely related to the g-transformation of Chapter 10.
Some Points of Grammar and Style
Published in Edward J. Rothwell, Michael J. Cloud, Engineering Writing by Design, 2020
Edward J. Rothwell, Michael J. Cloud
Fix each run-on sentence. We treated an analogous system in Chapter 1 the method of solution is the same.We refer to expressions of this type as waves additional waves are treated later in this section.To evaluate x, multiply (1) through by y and integrate this gives x = 2/c.Equation (2) is the f-transformation it is closely related to the g-transformation of Chapter 10.
Statistical Machine Translation and Comparable Corpora
Published in Krzysztof Wołk, Machine Learning in Translation Corpora Processing, 2019
RUN-ON—Also known as fused sentences, run-on sentences are typified by putting two complete sentences together without a break or punctuation. An example of a run-on sentence is: My favorite movies are documentaries they are very educational
Hybrid gene regulatory network for product styling construction in interactive evolutionary design
Published in Journal of Engineering Design, 2023
Dong Zeng, Jingjing Miao, Chaogang Tang, Yaxin Long, Maoen He
The population size is set to 12, crossover rate is 0.6, mutation rate is 0.03 and number of satisfactory schemes obtained by the user is 4. The extracted information from the H-PGRN provides inherent knowledge of the algorithm as follows: in order to retain the good traits, the important genes and gene association groups within the parents should be passed on as much as possible. Since unspecified features of the previous generation may affect the operation of the new generation during the fusion design of the system (Nie, Zhang, and Wang 2023), an inheritance based strategy is introduced here, as shown in Figure 11. Different inheritance paths are established according to the importance of the genes and the degree of connectivity within the associated groups. In this study, the probability of inheritance of association groups and is set to 0.8, and to 0.7, and to 0.6. After a trial run, it was found that there are about 6 genes with a high gene expression level, so the top six important gene nodes are selected to set the probability of inheritance to 0.8.
Effects of cadmium stress on the morphology, physiology, cellular ultrastructure, and BvHIPP24 gene expression of sugar beet (Beta vulgaris L.)
Published in International Journal of Phytoremediation, 2023
Dali Liu, Zhuo Gao, Jiajia Li, Qi Yao, Wenbo Tan, Wang Xing, Zhenqiang Lu
Specific primers were designed based on the BvHIPP24 gene (XM_010694287.2) in B. vulgaris [forward primer (5′-3′): GCT CTT TCT GGA CTT AAA GGA GTG; reverse primer (5′-3′): TGC CAC CAT AGT GTA AGG CA]. The product length was 162 bp. The RNA of B. vulgaris was extracted with RNA-easy Isolation Reagent (Vazyme), and reverse transcription synthesis of first-strand cDNA was performed using TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen). qRT-PCR was performed using TIANGEN SuperReal PreMix Plus (SYBR Green). The reactions were carried out as follows: 15 min denaturation at 95 °C, followed by 40 cycles of amplification (95 °C for 10 s, 60 °C for 32 s). The B. vulgaris glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene [forward primer (5′-3′): GCT TTG AAC GAC CAC TTC GC; reverse primer (5′-3′): ACG CCG AGA GCA ACT TGA AC] with an amplified product of 132 bp was used as a control. Each sample was run in triplicate, and the gene expression was statistically analyzed using the 2−△△Ct method (Livak and Schmittgen 2001).
Effect of Benzophenone-3 on performance, structure and microbial metabolism in an EGSB system
Published in Environmental Technology, 2020
Laura Castrillón Cano, Yudy Andrea Londoño, Nancy J. Pino, Gustavo A. Peñuela
cDNAs were used for the quantification of the mcrA, ACAs and gyrb genes mRNA levels by qRT-PCR with a LightCycler 1.5 (Roche, Switzerland). qRT-PCR was carried out using a LightCycler 480 SYBR Green I Master (Roche, Germany), in a 20 μL volume. The pair of mcrA. ACAs and and gyrB gene primers used have been reported previously [32–34]. Thermal cycling conditions were: 95°C for 10 min; followed by 45 cycles of 95°C for 10 s; 60°C for 20 s (ACAs gene); 55°C for 20 s (mcrA gene); 50°C for 20 s (gyrB gene); and a final extension step at 72°C for 30 s. A log 10 series of triplicate dilutions of DNA fragment containing reference gene were evaluated and the PCR efficiencies were determined from the slope of the curve. The correlation coefficients were also calculated from the curves. A melting curve was also done for every pair of primers to evaluate their specificity. Distilled water was included in each run as negative control, gyrb was used as housekeeping gene. The expression of each gene was measured in triplicate, and the relative expression ratio was calculated by the Pfaffl method.