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Bioconversion of Waste Biomass to Bioethanol
Published in Prakash Kumar Sarangi, Sonil Nanda, Bioprocessing of Biofuels, 2020
Prakash Kumar Sarangi, Sonil Nanda
Several microorganisms including fungi (e.g. Aspergillus, Candida shehatae, Fusarium sp., Kluyveromyces sp., Neurospora sp., Phanerochaete sp., Penicillium sp., Pichia kudriavzevii, Saccharomyces cerevisiae, Schizophyllum sp., Sclerotium sp., Trichoderma sp., etc.) and bacteria (e.g. Acetovibrio sp., Bacillus sp., Clostridium thermocellum, Erwinia sp., Escherichia coli, Klebsiella oxytoca, Ruminococcus sp., Zymomonas mobilis, etc.) accomplish fermentation of biomass hydrolysates to produce ethanol (Nanda et al. 2014b). S. cerevisiae is a model microorganism for ethanol fermentation because of its high efficiency, stability, a faster rate of sugar conversion and high solvent (alcohol) tolerance. Moreover, it is also considered as GRAS (generally regarded as safe). S. cerevisiae is also a potential producer of zymase, an enzyme complex that manifests the biocatalysis of sugar fermentation into ethanol and CO2 (Lin and Tanaka 2006).
Alcohol Fuels
Published in M.R. Riazi, David Chiaramonti, Biofuels Production and Processing Technology, 2017
Elia Tomás-Pejó, Antonio D. Moreno, M.R. Riazi, David Chiaramonti
With the aim of designing better engineering strategies, nonconventional microorganisms such as the native xylose-fermenting yeasts Spathaspora passalidarum, Scheffersomyces stipitis, or Candida sp. are under investigation to elucidate crucial physiological mechanisms for optimal pentose conversion to ethanol (Petschacher et al. 2005, Su et al. 2015). In addition, strains showing high tolerance to relatively high temperatures (Kluyveromyces marxianus, Ogataea polymorpha), high osmotic pressures (Zygosaccharomyces rouxii), high concentrations of inhibitory compounds such as acetic acid or furans (Zygosaccharomyces bailii, Wickerhamomyces anomalus, Pichia kudriavzevii, Candida stellata), and high concentration of ethanol (Dekkera bruxellensis, P. kudriavzevii, Schizosaccharomyces pombe, Torulaspora delbrueckii, and W. anomalus) are being studied (Tomás-Pejó et al. 2009, Miller and Blum 2010, Koppram et al. 2014, Radecka et al. 2015, Lindahl et al. 2016).
Challenges and Perspectives on Application of Biofuels in the Transport Sector
Published in Ayerim Y. Hernández Almanza, Nagamani Balagurusamy, Héctor Ruiz Leza, Cristóbal N. Aguilar, Bioethanol, 2023
F. G. Barbosa, S. Sánchez-Muñoz, E. Mier-Alba, M. J. Castro-Alonso, R. T. Hilares, P. R. F. Marcelino, C. A. Prado, M. M. Campos, A. S. Cardoso, J. C. Santos, S. S. Da Silva
In biodiesel production, LCB can be hydrolyzed and used for lipids synthesis by oleaginous yeast, such as Meyerozyma guilliermondii and Pichia kudriavzevii or filamentous fungus-like Mortierella isabellina for subsequent obtaining of biodiesel [19, 134, 135]. Those microorganisms could accumulate up to 60–80% lipids of their cell dry weight and can grow exponentially while utilizing cheap substrates, becoming suitable candidates for biotechnological purposes [136, 137]. The use of cheap carbon sources like crop byproducts, significantly diminishes the cost of yeast oil production [109].
A novel kinetic model for a cocoa waste fermentation to ethanol reaction and its experimental validation
Published in Preparative Biochemistry & Biotechnology, 2023
Eduardo Alvarado-Santos, Ricardo Aguilar-López, M. Isabel Neria-González, Teresa Romero-Cortés, Víctor José Robles-Olvera, Pablo A. López-Pérez
Sequences were submitted to a similarity search against NCBI database sequences entries using the BLAST program.[34] Sequence alignments and phylogenetic analysis were performed using MEGA version 6 and then analyzed using NCBI hyperlink (http://www.ncbi.nih.gov/). A neighbor-joining (NJ) tree was generated using Kimura’s two-parameters model and bootstrapped using 1000 random replicates with the MEGA (v 6.0) software package.[35] The Pichia kudriavzevii (KC765127), lyophilized strain, was rehydrated with sterile distilled water, subsequently, successive reseedings were made on agar plates containing 20 g/L peptone, 10 g/L yeast extract, 20 g/L xylose, and 20 g/L agar.
Effect of nickel oxide nanoparticles on bioethanol production by Pichia kudriavzveii IFM 53048 using banana peel waste substrate
Published in Environmental Technology, 2023
Florence Obiageli Nduka, Ikechukwu Noel Emmanuel Onwurah, Chikodili Joseph Obeta, Ekene John Nweze, Chinelo Chinenye Nkwocha, Favor Ntite Ujowundu, Ozoemena Emmanuel Eje, Juliet Onyinye Nwigwe
The isolate was identified as Pichia kudriavzevii and the morphological features of the selected yeast colonies were identified based on the growth on YEPD media. Also, the presence of yeast was confirmed by colony morphology, and microscopic observations in Plate 2, which were recorded in the primary isolation, are shown in Table 2.