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Microfluidics Devices as Miniaturized Analytical Modules for Cancer Diagnosis
Published in Raju Khan, Chetna Dhand, S. K. Sanghi, Shabi Thankaraj Salammal, A. B. P. Mishra, Advanced Microfluidics-Based Point-of-Care Diagnostics, 2022
Niraj K. Vishwakarma, Parul Chaurasia, Pranjal Chandra, Sanjeev Kumar Mahto
A liquid electrode DEP device was developed for continuous separation of particles of different conductivity. The device is advantageous in terms of its low cost because there is no use of expensive metal electrodes; additionally, it does not require a high voltage field for operation.120 To complete the circuit, an ionic liquid was used instead of metal electrodes in the PDMS device. The device performance was measured by continuous separation of PC-3 human prostate cancer cells. DEP force separates the particles in the electrode region due to the fact that different particles have different electrical properties and sizes. Ionic liquid and cells do not contact each other directly, and cells are collected in the culture medium. Moreover, the device is also capable of separating human breast cancer cells (MDA-MB-231) with high purity from stem cells (ADSCs). There are no metal electrodes, hence the fabrication is simpler and inexpensive.
Methanolic extract of Teucrium persicum up-regulates and induces the membrane restoration of E-cadherin protein in PC-3 cells
Published in International Journal of Environmental Health Research, 2023
Majid Tafrihi, Anahita Naeimi, Fatemeh Eizadifard
PC-3 cells are highly invasive and metastatic with a mesenchymal phenotype that is appropriate for anticancer agents (Tai et al. 2011). Our previous study showed that the treatment of PC-3 cells with methanolic extract of T. persicum leads to membrane localization of β-catenin protein and induction of epithelial-like morphology (Barati et al. 2021). Regarding the β-catenin as a partner of E-cadherin protein in adherence junctions (Zhu et al. 2020), we decided to evaluate the effect of T. persicum methanolic extract on the expression and cellular localization of E-cadherin protein in PC-3 cells.