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Carbon Nanotubes for Drug Delivery Applications
Published in Ann Rose Abraham, Soney C. George, A. K. Haghi, Carbon Nanotubes, 2023
Jahanvee Mitra, G. K. P. Srilekha, Nilesh Wagh, Jaya Lakkakula
Multidrug resistance (MDR) is a major drawback related to chemotherapy which leads to an increased dosage of the anticancer drug. An experiment was conducted in which an antibody P-gp was functionalized with SWCNTs (water-soluble) against the K562 cells (leukemia) aiming at the development of a target-specific DDS to overcome MDR. The loading and release of the DOX were observed by FT-IR spectroscopy and the characteristics of antibody-conjugated SWCNTs were measured by SEM and TEM. It was observed that Antibody P (Ap) increased the surface area of the CNTs, thus the loading capacity was increased and also that the Ap-SWCNTs efficiently targeted and entered the leukemia cells through endocytosis. Li et al. reported that the DDS system developed with Ap– SWCNTs successfully delivered the drugs and caused a high amount of cell cytotoxicity onto K562R cells, which could overpower the efflux of DOX by MDR. The best and highly demanding antibody-conjugated SWCNTs was established mainly to overcome the MDR and also to enhance the targeted delivery of DOX.70
Environmental and Biological Applications of Nanoparticles
Published in Sunipa Roy, Chandan Kumar Ghosh, Chandan Kumar Sarkar, Nanotechnology, 2017
Kaushik Roy, Chandan Kumar Ghosh, Chandan Kumar Sarkar
Though researchers are trying to develop efficient cancer treatment and therapies, drug resistance is considered to be one of the major difficulties in cancer treatment. Drug-coated polymer nanoparticles and nanospheres were explored to assess their ability to inhibit drug resistance by Wang et al. (2007). In this study, the researchers adopted a novel strategy for inhibiting multidrug resistance of tumor cells by adding tetraheptyl-ammonium-capped ferroso ferric oxide (Fe3O4) nanoparticles with accumulation of daunorubicin, a well-known anticancer drug. The result indicates the amazing synergistic effect of magnetic Fe3O4 nanoparticles on the uptake of daunorubicin in leukemia cells. The interaction between bioactive molecules and magnetic Fe3O4 nanoparticles on the cell membrane of leukemia cells probably contributes to the improved effect of cellular uptake, leading to enhanced synergistic effect to inhibit the drug resistance of leukemia K562 cells.
Optical Tweezers
Published in Yubing Xie, The Nanobiotechnology Handbook, 2012
Yingbo Zu, Fangfang Ren, Shengnian Wang
The basic use of optical tweezers in cell related research is to trap cells and position them at desired locations. As such, grabbing and relocating does not require contact with a solid surface or other cells, it is convenient to create appropriate model systems for some fundamental studies. For example, a single kidney cell was optically trapped to reveal the water transport across the cell membrane and correspondingly the volume change dynamics under osmotic shock (Lucio et al. 2003). Single K562 cells (a chronic leukemia cell line) had been positioned at various locations between two electrodes to study the cell membrane permeability change during and after an electric pulse to help understand the cell electroporation mechanism (Figure 19.8).
Enhanced periplasmic expression of human activin A in Escherichia coli using a modified signal peptide
Published in Preparative Biochemistry & Biotechnology, 2020
Zahra Hajihassan, Niloofar Khairkhah, Farshid Zandsalimi
The functional activity of activin A may be assessed by determining its ability to stimulate differentiation of cultured K562 erythroleukemia cells. This activity can be monitored by measuring the accumulation of hemoglobin according to the procedures described by Schwall and Lai but with some modifications.[22] Briefly, K562 cells were cultured in RPMI-1640 medium containing 10% heat-inactivated fetal calf serum along with penicillin and streptomycin. To induce erythroid differentiation, 50 and 100 ng/mL of recombinant activin A were individually added to the plate and incubation was done at 37 °C for 72 hr. Finally, the count of Fe+2 ions was measured with inductively coupled plasma optical emission spectrometry (Varian 730-ES Axial ICP-OES, Varian Medical Systems, Pal Alto, CA).
Folate receptor-specific cell-cell adhesion by using a folate-modified peptide-based anchor
Published in Journal of Biomaterials Science, Polymer Edition, 2019
Hiroko Nagai, Wataru Hatanaka, Masayoshi Matsuda, Akihiro Kishimura, Yoshiki Katayama, Takeshi Mori
K562 cells were maintained on suspension culture dishes (Greiner Bio-One, Munich, Germany) in RPMI-1640 medium (FUJIFILM Wako Pure Chemicals) supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U/mL penicillin, 100 μg/mL streptomycin and 0.25 μg/mL amphotericin B (all from Invitrogen, Grand Island, USA). Cells were cultured in a humidified atmosphere containing 5% CO2 at 37 °C. KB cells were maintained on cell culture dishes (Greiner Bio-One, Munich, Germany) in E-MEM medium (FUJIFILM Wako Pure Chemicals) supplemented with 10% (v/v) FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL amphotericin B, 2 mM L-Glutamin (FUJIFILM Wako Pure Chemicals). Cells were incubated in a humidified atmosphere containing 5% CO2 at 37 °C.
Green, lipophilic and recyclable catalysts for the aerobic oxidation of alcohols
Published in Inorganic and Nano-Metal Chemistry, 2020
Zahra Mousavi, Bahram Pourgholam, Asghar Zamani, Seyyed Meysam Abtahi Froushani
Cell culture is the first step to identify, assess, and prioritize chemical agents and natural products with the aim of anti-cancer effects.[26] K562 cells are of the erythroleukemia cell line. These non-adherent cells established from the pleural effusion of 53-year-old female with Chronic Myelogenous Leukemia (CML) in blast crisis.[27]