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Health and safety
Published in Roger Timings, Fabrication and Welding Engineering, 2008
The basic requirements for safe working are to: Learn the safe way of doing each task. This is also the correct way.Use the safe way of carrying out the task in practice.Ask for instruction if you do not understand a task or have not received previous instruction.Be constantly on your guard against careless actions by yourself or by others.Practice good housekeeping at all times, e.g. keep your working area clean and tidy and your tools and equipment in good condition.Co-operate promptly in the event of an accident or a fire.Report all accidents to your instructor or supervisor.Draw your instructor’s or your supervisor’s attention to any potential hazard you have noticed.
Metals I: Metals Preparation and Manufacturing
Published in Ronald Scott, of Industrial Hygiene, 2018
Good housekeeping is an important part of maintaining a safe work environment. Clothing can become heavily contaminated. Cleanup should be done with a thorough understanding of the nature of the dusts. Sweeping can add quantities of a fine particulate to the air and so should be replaced by vacuuming. Where very fine particulate is present, the vacuum should be equipped with a high-efficiency (HEPA) filter. Hosing an area with water can allow reactions between oxides and water to produce harmful gases. Furnace burners should be cleaned with care to prevent exposure to vanadium oxide residues from the fuel.
Construction management in practice
Published in Fred Sherratt, Peter Farrell, Introduction to Construction Management, 2023
Facilitating good housekeeping is an important part of site health and safety management – by making it easy for people to maintain a clear work area many hazards and risks will be avoided. Mess and rubbish also attracts other mess and rubbish, so even small piles of waste should be cleared up immediately by those who made them. A clean and tidy site is more likely to stay that way.
Neuroprotective effect of quercetin through targeting key genes involved in aluminum chloride induced Alzheimer’s disease in rats
Published in Egyptian Journal of Basic and Applied Sciences, 2023
Hala A Elreedy, Asmaa M. Elfiky, Asmaa Ahmed Mahmoud, Khadiga S. Ibrahim, Mohamed A Ghazy
The qRT-PCR was carried out in duplicate for each sample using Maxima SYBR Green qPCR Master Mix, Thermo Scientific. The reaction mixture was prepared in a total volume of 20 μL containing 10 μL of 2× SYBR Green Master Mix, 0.5 μL of each primer set for each gene and 4.5 μL of cDNA (100 ng/μL) and completed to 20 μL with water (nuclease-free). The housekeeping gene (GAPDH) was utilized to normalize each gene expression. The primer sequences for various genes (synthesized by Biosearch Technologies, USA) according to the previous study [18] and illustrated in table 1. PCR amplification was applied as the following protocol: 95°C for 10 min and 40 cycles of 95°C for 15 s, annealing for 30 s as appropriate for every primer set’s melting temperature, extension at 72°C for 10 s. RT‑qPCR was conducted using the 7500 Real‑Time PCR system (Applied Biosystems) in NRC lab. Relative expression levels of mRNA were analyzed using the 2−ΔΔCT formula.
Scaffold geometry and computational fluid dynamics simulation supporting osteogenic differentiation in dynamic culture
Published in Computer Methods in Biomechanics and Biomedical Engineering, 2023
Somruethai Channasanon, Pakkanun Kaewkong, Surapol Chantaweroad, Passakorn Tesavibul, Yotsakorn Pratumwal, Somboon Otarawanna, Soshu Kirihara, Siriporn Tanodekaew
To determine the level of osteoblast-specific gene expression, i.e. type I collagen, osteopontin and osteocalcin, total RNA was extracted from cell-seeded scaffold using TRIZOL reagent (Life Technologies, USA) according to the manufacturer’s instruction. First-strand complementary DNA (cDNA) was synthesized from 1 µg RNA by using RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, USA). The following amplification primers were used: type I collagen forward primer: 5′- TCTCCACTCTTCTAGTTCCT-3′; reverse primer:5′- TTGGGTCATTTCCACATGC-3′, osteopontin forward primer: 5′-CTTTCACTCCAATCGTCCCTAC-3′; reverse primer:5′-GCTCTCTTTGGAATG CTCAAGT-3′, osteocalcin forward primer: 5′-ATGAGGACCCTCTCTCTGCT-3′; reverse primer: 5′-CCGTAGATGCGTTTGTAGGC-3′, GAPDH forward primer: 5′-GCCATCAACGACCCCTTCATTGAC-3′; reverse primer: 5′-ACGGAAGGCCATGCC AGTGAGCTT-3′. Real-time PCR reactions were prepared by using Fast SYBR® Green Master Mix (Applied Biosystems, USA) and performed by using 7500 Fast Real-Time PCR system (Applied Biosystems). Target gene expression was normalized to the expression level of housekeeping gene GAPDH. The relative amount of each gene was calculated using the comparative 2-ΔΔCt method.
Macro- and microporous polycaprolactone/duck’s feet collagen scaffold fabricated by combining facile phase separation and particulate leaching techniques to enhance osteogenesis for bone tissue engineering
Published in Journal of Biomaterials Science, Polymer Edition, 2022
Youngeun Song, Joo Hee Choi, Nomin-Erdene Tumursukh, Na Eun Kim, Ga Young Jeon, Se Eun Kim, Soo In Kim, Jeong Eun Song, Yaşar Murat Elçin, Gilson Khang
RT-PCR was applied to evaluate osteogenic gene expression. The cell-laden scaffolds were cultured for 3 and 14 days and at a specific time point, the scaffolds were stored in a deep-freezer (- 80 °C). After all the samples were ready, they were thawed and treated with Trizol (Takara Bio, Inc., Japan) to induce cell lysis. Thereafter, chloroform (SAMCHUN chemicals, South Korea) was mixed and centrifuged at 12000 rpm at 4 °C for 15 min. The supernatant was transferred into a new Eppendorf tube (EP tube). 2-propanol (Sigma-Aldrich, USA) was added to the solution and stored at − 20 °C overnight. On the next day, samples were centrifuged at 12000 rpm at 4 °C for 15 min and the supernatant was removed and the obtained RNA was washed with an ice-cold 75% ethanol and centrifuged at 7500 rpm at 4 °C for 5 min. The supernatant was removed and RNase-DNase-free water (Gibco, USA) was added to dilute the obtained mRNA. The concentration was measured with a Biospectrophotometer (Eppendorf, USA) and cDNA was synthesized with a TOPscript™ RT DryMIX (dT18 plus) (enzynomics, South Korea) and PCR thermocycler (Takara Bio, Inc., Japan) according to the protocol. For RT-PCR, SYBRTM Green PCR Master Mix (Applied Biosystems, USA) and StepOnePlus Real-Time PCR system (Applied Biosyctems, USA) were used. Osteogenesis-specific genes: ALP; collagen type 1 (COL1); osteocalcin (OCN); runt-related transcription factor 2 (RUNX2) were used in this study and GAPDH was applied as a housekeeping gene. The sequences of the primers are described in Table 2.