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Glossary of scientific and technical terms in bioengineering and biological engineering
Published in Megh R. Goyal, Scientific and Technical Terms in Bioengineering and Biological Engineering, 2018
Synapomorphy is a shared derived trait between evolutionary lineages. A homology that evolved in an ancestor common to all species on one branch of a phylogeny, but not common to species on other branches.
Synthesis of oxidovanadium complexes containing coumarin and naphthalene: bromoperoxidase activity and DNA/BSA binding
Published in Journal of Coordination Chemistry, 2023
Mitali Majumder, Tapashi Das, Kajal Krishna Rajak
DNA is assumed to be the primary target for cytotoxicity [15]. Vanadium metal complexes bind with DNA mainly through covalent and non-covalent interactions, such as major and minor groove binding, intercalation and electrostatic interactions [16]. There is also a possibility that the interaction with various metal complexes leads to destruction of DNA which in turn helps to terminate cancerous cells. In biological fluids, there is a major contribution of serum albumin in circulatory system and it has importance in physiological and pharmacological functions. As Bovine serum albumin (BSA) contains 76% sequence homology with Human serum albumin (HSA) [17], it can be taken as a model protein. So, BSA binding study with metal complexes is also important for the implementation of drug delivery.
The response surface optimization of β-mannanase produced by Weissella cibaria F1 and its potential in juice clarification
Published in Preparative Biochemistry & Biotechnology, 2022
Hairui Ji, Huiying Cao, Li Zhao, Ruiying Na, Wenxiang Ping, Jingping Ge, Dan Zhao
The genomic DNA prep Pure Bacteria Kit (Tiangen Biotech Co. Ltd., China) was used to extract genomic DNA of β-mannanase-producing LAB. The polymerase chain reaction (PCR) amplification primers (Shenggong Biotech Co. Ltd., China) were the universal primers for specific identification, 16 SF (5′-AGAGTTTGATCCTGGCTCAG-3′) and 16 SR (5′-CTACGGCTACCTTGTTACGA-3′). The PCR conditions included a first denaturing steep for 3 min at 95 °C, followed by 30 cycles: denaturing for 30 s at 95 °C, annealing for 45 s at 55 °C, extension for 90 s at 72 °C and final extension for 10 min at 72 °C.[21] The obtained amplicons were confirmed with agarose gel electrophoresis 1.0% (w/v). The sequence homology was analyzed by blast in National Center for Biotechnology Information (NCBI) database. Different strains were selected, according to the sequence homology. The phylogenetic tree was constructed by MEGA 5.0 software to determine the species relationship.
Lindane degradation by root epiphytic bacterium Achromobacter sp. strain A3 from Acorus calamus and characterization of associated proteins
Published in International Journal of Phytoremediation, 2019
Unique polypeptides appeared in lane corresponding to MSM supplemented with lindane and these bands were not observed in the culture without lindane (Figure 3). Approximate molecular weight of unique polypeptide bands was estimated using Molecular Weight Analysis Tool of Image LabTM software (version 3.0) using BlueRay prestained protein ladder (10-180kD, GeneDireXTM) as standard molecular weight marker. The estimated molecular weight of polypeptides for Achromobacter sp. strain A3 was 35.5kD and 64.7kD for band 1 and 2, respectively. For identification, proteins were digested with trypsin and subjected to MALDI-TOF and MS/MS analysis. They were identified using elucidated amino acid sequence by sequence homology. Mass spectra of polypeptides revealed intense peaks which were subjected to Mascot Search against the NCBI database. Table 2 shows the matched protein for each corresponding protein band of Achromobacter sp. strain A3 with significant protein score and sequence coverage.