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Hydrogels with Ubiquitous Roles in Biomedicine and Tissue Regeneration
Published in Harishkumar Madhyastha, Durgesh Nandini Chauhan, Nanopharmaceuticals in Regenerative Medicine, 2022
Priyanka, Pooja A Chawla, Aakriti, Viney Chawla, Durgesh Nandini Chauhan, Bharti Sapra
In dentistry, root canal therapy (RCT) is commonly performed. During RCT, the removal of tender or necrotic dental pulp is carried out followed by replacement with a synthetic material. On the contrary, current investigations provide substantial data on the possibility of dental pulp and dentin engineering. As TE approach holds the promise to override conventional RCT, there is a need for customised scaffolds for the same. Various investigations in recent times have demonstrated the capability of dental pulp stem cells (DPSCs) along with a scaffold material to produce soft connective tissue, which is analogous to dental pulp (Cordeiro et al., 2008; Huang et al., 2010; Iohara et al., 2009; Nakashima and Iohara, 2011; Prescott et al., 2008; Sakai et al., 2010).
Clinical Progresses in Regenerative Dentistry and Dental Tissue Engineering
Published in Vincenzo Guarino, Marco Antonio Alvarez-Pérez, Current Advances in Oral and Craniofacial Tissue Engineering, 2020
The first type of dental pulp stem cell was isolated from the human pulp tissue and termed as ‘post-natal’ Dental Pulp Stem Cells (DPSCs) by Gronthos et al. 2000. The stem cell population in the pulp is tiny: approximately 1% of the total cells (Smith et al. 1995).
Large three-dimensional cell constructs for tissue engineering
Published in Science and Technology of Advanced Materials, 2021
Jun-Ichi Sasaki, Gabriela L Abe, Aonan Li, Takuya Matsumoto, Satoshi Imazato
Dental pulp assumes a variety of roles including maintenance of tooth homeostasis, pain transmission, and regeneration of dentin [84,85]. Generally, dental pulp is extracted in the case of irreversible pulpitis and pulp necrosis, then the root canal is filled with a rubber-based material [86,87]. However, pulpless teeth lose their natural biological defense, increasing the risk of serious caries, apical periodontitis, and ultimately tooth loss [88,89]. Specifically, the hazard ratio for tooth loss increases 7.4-fold for pulpless molars and 1.8-fold for pulpless anterior teeth and premolars relative to their pulp-conserved counterparts [90]. Thus, dental pulp regeneration can recover the function of teeth and improve the prognosis of a pulpless tooth. 3D cell constructs were fabricated using dental pulp stem cells (DPSCs) and their ability to regenerate dental pulp was evaluated [91].
Dental pulp stem cells in serum-free medium for regenerative medicine
Published in Journal of the Royal Society of New Zealand, 2020
Dawn E. Coates, Mohammad Alansary, Lara Friedlander, Diogo G. Zanicotti, Warwick J. Duncan
Research into the growth and clinical applications of dental pulp stem cells (DPSCs) has increasing focused on serum-free options for expanding and differentiating these cells. There is clear data that different serum-free media will change the behaviour of DPSCs when cultured for extended time periods (Bakopoulou et al. 2017). A definition of what constitutes ‘current good practice’ in relation to in vitro amplification of DPSCs and an agreement on the markers that define DPSCs is required. This review aims to investigate key research on DPSCs in serum-free media and ascertain if there is any consistency in the culture media being used and the MSC markers employed.