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Reduction and Fixation of Sacroiliac joint Dislocation by the Combined Use of S1 Pedicle Screws and an Iliac Rod
Published in Kai-Uwe Lewandrowski, Donald L. Wise, Debra J. Trantolo, Michael J. Yaszemski, Augustus A. White, Advances in Spinal Fusion, 2003
Kai-Uwe Lewandrowski, Donald L. Wise, Debra J. Trantolo, Michael J. Yaszemski, Augustus A. White
Within platelets are a variety of bone growth factors: TGF-β, platelet-derived growth factors (PDGF), IGF, and vascular endothelial growth factors (VEGF). Scientists have discovered that with a concentration of 1 million platelets/μL, enough growth factors can be isolated to enhance bone growth [14]. In addition, fibrinogen, which is found within the plasma containing the platelets, is used in this solution to aid in homeostasis. The formation of such solution is a multistep process [1,14]: To begin with, one unit of blood is drawn from the patient before surgery. Using a cell saver, this blood is separated into three parts: red cells, plasma, and a buffy coat. The buffy coat is taken and ultra-concentrated to a density of 1 million platelets/μL. This concentrate is then added to thrombin to make it into a soft or hard gel, depending on the concentration of thrombin added. The gel is then cut into strips and added to the fusion site as needed.
Magnetic Separation in Integrated Micro-Analytical Systems
Published in Nguyễn T. K. Thanh, Clinical Applications of Magnetic Nanoparticles, 2018
Separation of cells can be categorized into two types depending on whether the method uses biochemical assay, which relies on biochemical binding, including antigen–antibody binding (immunoassay), biotin–avidin binding and DNA and RNA hybridization. Physical separation does not depend on biochemical binding. The most commonly utilized method is centrifugation to separate cells based on their density. After centrifugation of blood, cells are sorted in different layers. Leukocytes (white blood cells), thrombocytes (platelets) and possible cancer cells are separated into a thin layer called a buffy coat between the layers containing erythrocytes (red cells) and plasma. Commonly utilized types of separation media include Ficoll and OncoQuick.60
Green, lipophilic and recyclable catalysts for the aerobic oxidation of alcohols
Published in Inorganic and Nano-Metal Chemistry, 2020
Zahra Mousavi, Bahram Pourgholam, Asghar Zamani, Seyyed Meysam Abtahi Froushani
This experimental study was granted institutional ethical permission by Urmia University, Urmia, Iran. Heparinized blood samples (10 mL) were isolated from 5 volunteers. All volunteers signed the consent form. Samples were centrifuged for 10 min at 400 g at 4 °C to isolate a buffy-coat layer. The collected cells were diluted 1: 2 in PBS and centrifuged over Histopaque 1083 for 30 min at 800 g at 18 °C. The cells were re-suspended and contaminant erythrocytes were deleted by hypotonic lysis. PBMCs were rinsed three times and then re-suspended in RPMI-1640 containing 10% fetal calf serum.