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Published in Chad A. Mirkin, Spherical Nucleic Acids, 2020
Samuel A. Jensen, Emily S. Day, Caroline H. Ko, Lisa A. Hurley, Janina P. Luciano, Fotini M. Kouri, Timothy J. Merkel, Andrea J. Luthi, Pinal C. Patel, Joshua I. Cutler, Weston L. Daniel, Alexander W. Scott, Matthew W. Rotz, Thomas J. Meade, David A. Giljohann, Chad A. Mirkin, Alexander H. Stegh
All animals were used under an approved protocol of the Institutional Animal Care and Use Committee of Northwestern University. To implant tumors, we suspended U87MG and a patient-derived TNS line in Hanks balanced salt solution. Each mouse was anesthetized and placed in a stereotaxic frame, and the surgical area was cleaned with alcohol and Betadine. For these studies, the cells were implanted in ~7-week-old female CB17 SCID mice (Taconic Farms). An incision was made in the scalp, and then a 0.7-mm burr hole was created in the skull with a microsurgical drill 2 mm lateral right of the sagittal suture and 0.5 mm posterior of bregma. A Hamilton syringe was loaded with either 1 × 105 U87MG cells or 2 × 105 huTNS cells and inserted 3.5 mm into the brain. The cells were implanted over a period of 5 min, and the needle was left in place for 1 min before withdrawing the syringe. After surgery, the skin was closed with sutures.
Cognition impairment of rat in undersea environment
Published in International Journal of Environmental Health Research, 2022
Yingxin Zou, Ying Tang, Wei Fan, Lina Liu, Yong Jiao
After the simulation model process, rats were immediately placed in the prone position on stereotaxic head holder under anesthesia with sodium pentobarbital. Approximately 3 mm posterior and 3.5 mm lateral to the bregma of rat brain, a burr hole was drilled through the skull to expose the middle cerebral artery, and a fiber optic probe (POF500, Moor Instruments Ltd., United Kingdom) was fixed to it by glue. The probe was then connected to a laser-Doppler blood flow survey meter (VMS-LDF1, Moor Instruments Ltd., United Kingdom). After the baseline of CBF stabile, accumulated CBF data in 5, 10, and 15 min was recorded, respectively. The time spent from the ending of the model process to the beginning of data acquisition was limited to 10 min. The CBF signal was calculated by transferring to the perfusion unit (PU).
The protective mechanism underlying phenylethanoid glycosides (PHG) actions on synaptic plasticity in rat Alzheimer’s disease model induced by beta amyloid 1-42
Published in Journal of Toxicology and Environmental Health, Part A, 2018
Jian-xin Jia, Xu-sheng Yan, Wei Song, Xin Fang, Zhi-ping Cai, Dong-sheng Huo, He Wang, Zhan-jun Yang
Rats were anesthetized by intraperitoneal (ip) injection of 2% pentobarbital sodium. Cannulae were implanted in bilateral ventricles and attached to the skull surface using dental cement and jeweler’s screws. Based upon the atlas of the rat brain (Paxinos and Watson 1986), stereotaxic coordinates were: anterior-posterior (AP), 0.8 mm from bregma; medial-lateral (ML), ± 1.5 mm from the midline; and dorsal-ventral (DV), 3.7 mm from the skull surface. After 7 days, bilateral ventricles injections were administered through cannulae using injection needles connected by polyethylene tubing to 5μl Hamilton microsyringes. Five μl soluble Aβ 1-42 oligomers (2 μg/μl) was stereotaxically injected from the left for 5 min followed by 5 min from the right into bilateral ventricles at a rate of 1 μl/min.
Automatic three-dimensional cephalometric annotation system using three-dimensional convolutional neural networks: a developmental trial
Published in Computer Methods in Biomechanics and Biomedical Engineering: Imaging & Visualization, 2020
Sung Ho Kang, Kiwan Jeon, Hak-Jin Kim, Jin Keun Seo, Sang-Hwy Lee
The cephalometric landmarks for this study included nasion, bregma, foramen magnum (centre), menton, mandibular foramen, coronoid, orbitale and porion, the latter four points being bilateral (Supplementary Table 1). The former three points were used to determine the midsagittal plane, the latter two bilateral points to determine the Frankfort plane. We divided the landmarks into three groups based on their regional ranges to observe their possible relationship with the regional and anatomical characteristics of landmarks. The x-axis indicated the transverse dimension, the y-axis the anterior-posterior dimension and the z-axis the superior-inferior dimension.