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Nanotechnologies Assemblies of siRNA and Chemotherapeutic Drugs Codelivered for Cancer Therapeutic Applications
Published in Loutfy H. Madkour, Nanoparticle-Based Drug Delivery in Cancer Treatment, 2022
Therefore, the objective of this section study was to develop a topical siRNA delivery system that can permeate through the stratum corneum and viable epidermis and efficiently deliver BRAF-targeted siRNA to the basal epidermis where melanoma cells reside. The study [215] has been focused on designing a delivery system for topical administration of BRAF-siRNA (v-Raf murine sarcoma viral oncogene homolog B). Molecular analysis of melanoma cells from patients has shown that the majority of the melanocytes contain a mutation in the gene that encodes BRAF protein. Among BRAF mutations, 90% involves a single-point mutation that substitutes thymine with adenine at nucleotide 1799 [216]. This mutation in the gene-encoding BRAF protein activates the downstream signals of the mitogen-activated protein kinase pathway and ultimately causes an oncogenic increase of melanocytes proliferation and division [217]. Due to the high incidence of mutation in BRAF gene in melanoma cells and increased risk of mortality, we selected the BRAF-siRNA as the model therapeutic for delivery.
Trends in Cancer Screening: Different Diagnostic Approaches
Published in Anjana Pandey, Saumya Srivastava, Recent Advances in Cancer Diagnostics and Therapy, 2022
Anjana Pandey, Saumya Srivastava
Mutations in the BRAF gene can be present in melanoma (Glitza and Davies, 2014), colorectal, and thyroid cancer. Various assays are used for the detection of this mutation such as pyrosequencing (Romano, 2015).
Thyroid Disorder Diagnosis by Optimal Convolutional Neuron based CNN Architecture
Published in Journal of Experimental & Theoretical Artificial Intelligence, 2022
Rajole Bhausaheb Namdeo, Gond Vitthal Janardan
Musholt et al. (2010) have conducted real-time experimentation with 290 preoperatively harvested FNABs that underwent routine cytologic evaluation. The authors have performed the BRAF V600E mutation analysis through mutation-specific PCR. Then, a hybrid-specific RT-PCR assay was also utilised to detect the RET/PTC1 rearrangements. The identified genetic alterations were evaluated via direct sequencing.