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Bacteria Causing Gastrointestinal Infections
Published in K. Balamurugan, U. Prithika, Pocket Guide to Bacterial Infections, 2019
B. Vinoth, M. Krishna Raja, B. Agieshkumar
Laboratory diagnosis for V. cholerae involves biochemical or serological tests for the identification of the presence of O1 serogroup antigens. The subtyping of O1 serogroup as Inaba, Ogawa, and Hikojima could be done by agglutination test. Slide agglutination test is done by treating the cultures grown on heart infusion agar, Kligler’s iron agar, and triple sugar iron agar with the antiserum to detect the specific O antigen. Other than this, some of the biochemical tests like oxidase test, ring test, triple sugar iron test, carbohydrate test, decarboxylase test, and Voges-proskauer test are done infrequently based on the necessity. The hemolytic activity of V. cholerae is used to distinguish the biotypes such as classical and E1T; classical types show negative hemolytic activity, whereas E1T or of Australian or the US gulf coast strain shows strong positive hemolytic activity.
Serratia
Published in Dongyou Liu, Handbook of Foodborne Diseases, 2018
Naheed S. Kanji, Umesh Narsinghani, Ritu A. Kumar
There are several biochemical tests that help to identify Serratia in the laboratory. S. marcescens are negative for (1) the methyl red test due to their production of 2,3-butanediol and ethanol and (2) acid production on lactose. It is positive for (1) the Voges-Proskauer test, which shows an organism's ability to convert pyruvate to acetoin, glucose, and sucrose; (2) the citrate test to produce pyruvic acid; and (3) the decarboxylase test, which is the removal of a carboxyl group from an amino acid, producing an amine and carbon dioxide.49
Characterization of integrons, extended-spectrum β-lactamases, AmpC cephalosporinase, quinolone resistance, and molecular typing of Shigella spp. from Iran
Published in Infectious Diseases, 2018
Sajjad Zamanlou, Mohammad Ahangarzadeh Rezaee, Mohammad Aghazadeh, Reza Ghotaslou, Farhad Babaie, Younes Khalili
In a prospective descriptive study, from May 2014 to May 2015, a total of 142 non-duplicate Shigella isolates were obtained from the stool cultures of different patients with diarrhea or dysentery at Tabriz, Urmia, Ardabil (northwest), and Kerman (southeast) cities, in Iran. All isolates were identified using standard Enterobacteriaceae differentiation biochemical tests such as triple sugar iron agar, motility, indole production, urea hydrolysis, methyl red/voges-Proskauer test, lysine decarboxylation, and citrate utilization. Furthermore, all isolates were serotyped by the slide agglutination test using the commercial specific polyvalent antisera (SIFIN, GmbH Berlin, Germany) [16]. The confirmed Shigella isolates were kept in a tryptic soy broth containing 20% of glycerol at –70 °C for further study.
Characterization and applications of exopolysaccharide produced by marine Bacillus altitudinis MSH2014 from Ras Mohamed, Sinai, Egypt
Published in Egyptian Journal of Basic and Applied Sciences, 2018
Sahar S. Mohamed, Shaimaa K. Amer, Manal S. Selim, Hala M. Rifaat
Colonies of strain No. 12 showed mucoid appearance on solid medium. The cells were Gram positive, rod shaped, motile and spore forming when observed under phase contrast microscope. In nitrate reduction, Voges-Proskauer tests and acid production from glucose, catalase and oxidase testes, the strain was positive, while negative for gas production from glucose. Hence, strain No. 12 was temporary identified as Bacillus sp. particularly, the partially sequenced 16S rRNA genes showed 99% similarity with Bacillus altitudinis, so strain No. 12 was identified as Bacillus altitudinis MSH2014 with accession No. (KY550404) (Fig. 1).