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Order Blubervirales: Surface Protein
Published in Paul Pumpens, Peter Pushko, Philippe Le Mercier, Virus-Like Particles, 2022
Paul Pumpens, Peter Pushko, Philippe Le Mercier
Second, yeast Pichia pastoris was used for another set of hepatitis B vaccines. They were Cuban SHBs-based vaccine Heberbiovac HB elaborated by the Centro de Ingenieria Genetica Y Biotecnologia and produced by the Heberbiotec SA (Cuba) company (Galbán García et al. 1992) and its analogue, which was produced in India under the trademark Enivac HB (Kaur and Mani 2000; Estévez et al. 2007). A low-cost P. pastoris-produced vaccine Shanvac B was registered in India (Abraham et al. 1999).
Role of Engineered Proteins as Therapeutic Formulations
Published in Peter Grunwald, Pharmaceutical Biocatalysis, 2019
Khushboo Gulati, Krishna Mohan Poluri
Several of the protein therapeutics have already been developed using different protein scaffolds and are under clinical trials. Some of them include: Affibodies® from the synthetic Z domain of staphylococcal protein, Adnectins® from type III fibronectin domains, Avimers® from LDLR-A modules, Anticalins® from lipocalins, engineered Kunitz Domains, and DARPins® from ankyrin repeat proteins. One of the synthetic scaffolds, namely ecallantide (Kalbitor®), is a 60-amino-acid-long protein based on kunitz domain and has been approved as a therapeutic that inhibits plasma kallikrein. It was selected by phage display technology and was grown in Pichia pastoris strain of yeast (Zuraw et al., 2010). Some of these scaffolds are described in detail in the following sections.
Recombinant Antibodies
Published in Siegfried Matzku, Rolf A. Stahel, Antibodies in Diagnosis and Therapy, 2019
Melvyn Little, Sergey M. Kipriyanov
The methylotrophic yeast Pichia pastoris has been shown to be suitable for the high-level expression of various heterologous proteins, either intracellular or secreted into the culture supernatant (see review by Cregg et al., 1993). Recently, Ridder et al. (1995) demonstrated the applicability of the Pichia expression system for the secretion of antibody fragments. A rabbit scFv against the human leukemia inhibitory factor (hLIF) was expressed in Pichia cells using the expression vector pPIC9 that provides the α-mating factor signal sequence for secretion and the HIS4 gene for selection of the recombinant yeast clones. The yield was about 100 mg per liter of shake flask culture.
Prevention and Detoxification of Mycotoxins in Human Food and Animal Feed using Bio-resources from South Mediterranean Countries: a Critical Review
Published in Critical Reviews in Toxicology, 2023
Amina Aloui, Jalila Ben Salah-Abbès, Abdellah Zinedine, Amar Riba, Noel Durand, Jean Christophe Meile, Didier Montet, Catherine Brabet, Samir Abbès
Recently, Abdel-Kareem et al. (2019) have explored the action mechanism and bio-control ability of S. cerevisiae against the aflatoxigenic Aspergillus flavus. They noticed that all tested yeasts were able to inhibit the growth of Aspergillus flavus Z103, and that the maximum rate of elimination (85%)was recorded with living cells. The authors have suggested degradation as a mechanism of detoxification regarding the extensive production of exo-chitinase, which is an extracellular lytic enzyme. Moreover, Tayel et al. (2013) highlighted the ability of Pichia anomala to inhibit A. flavus growth in malfunctioning airtight storage systems. They suggested that the hyphae of A. flavus were totally penetrated and destroyed by the parasitic and lytic actions of the antagonistic yeast, which is the possible mode of action adopted by this antagonistic yeast against fungal growth. This could be proposed as a possible approach for the bio-control of mycotoxins since the yeast exerts a beneficial effect by suppressing fungal growth and limiting mycotoxin production.
Interactions among the mycobiome, bacteriome, inflammation, and diet in people living with HIV
Published in Gut Microbes, 2022
María José Gosalbes, Nuria Jimenéz-Hernandéz, Elena Moreno, Alejandro Artacho, Xavier Pons, Sonia Ruíz-Pérez, Beatriz Navia, Vicente Estrada, Mónica Manzano, Alba Talavera-Rodriguez, Nadia Madrid, Alejandro Vallejo, Laura Luna, José A. Pérez-Molina, Santiago Moreno, Sergio Serrano-Villar
PWH showed an altered mycobiome, in keep with that defined in other inflammatory diseases, such as Crohn’s disease, Clostridium difficile infection, or type 2 diabetes.28–32 All these diseases are characterized by an enrichment for Candida genus and specifically C. albicans. In our study, PWH showed enrichment for C. albicans, C. parapsilosis, and D. hansenii. In contrast, Malassezia restricta was depleted in PWH, while in Crohn’s disease this yeast dominates the mycobiome and correlates with disease severity.33Pichia kluyveri was also depleted in PWH, in accordance with Mukherjee et al.14 that described an enrichment of Candida and a depletion of Pichia in the oral cavity of PWH. Interestingly, Penicillium, which was also depleted in PWH mycobiome, has been described as a xenobiotic degrader resulting in anti-inflammatory metabolites.34
Effects of the Cobalt-60 gamma radiation on Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase
Published in International Journal of Radiation Biology, 2022
Abdelghani Iddar, Mohammed El Mzibri, Adnane Moutaouakkil
This work was carried out to assess the adaptive response of Pichia pastoris, used as a eukaryotic model, after gamma irradiation. Indeed, the methylotrophic yeast P. pastoris represents a good eukaryotic model for many studies. Similar to Escherichia coli, P. pastoris can be grown easily in a laboratory with a relatively short regeneration time and it is able to grow up to high cell densities, resulting in high protein production yields (Potvin et al. 2010). P. pastoris is a single cell organism considered as most effective heterologous expression system with an easy carry out of genetic manipulation and it has become more extensively used for industrial applications (Gasser et al. 2013). P. pastoris allowed the expression and production of proteins that could not be produced efficiently by bacteria or other host organisms (Cereghino et al. 2002). Moreover, P. pastoris has a number of promoters that allow the regulation of proteins in the cell (Stadlmayr et al. 2010). The purpose of this study was to evaluate the effect of Cobalt-60 gamma radiation on P. pastoris GAPDH activity and protein levels. The effect of radiation on induction of some oxidative damages and on antioxidant enzymes activities was also explored.