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Dermal Fibroblast Function
Published in Brian J. Nickoloff, Dermal Immune System, 2019
When fibroblasts are placed in culture they will aggregate independently from teratocarcinoma cells and from myoblasts.100,101 The cadherins which are Ca2+ dependent are very important in such selective cell-cell adhesion reactions.102 Three subclasses, E-, N-, and P-, of cadherins exist and all have a similar structure, consisting of a carboxy-terminal cytoplasmic domain, a transmembrane domain, and an extracellular amino-terminal domain.99 All cadherins have 43 to 58% amino acid homology.99 Although fibroblasts from mature animals do not usually express significant levels of cadherins, they may express certain of these during embryogenesis. N-cadherin is expressed on embryonic chick-liver fibroblasts.103
Angiogenesis and Roles of Adhesion Molecules in Psoriatic Disease
Published in Siba P. Raychaudhuri, Smriti K. Raychaudhuri, Debasis Bagchi, Psoriasis and Psoriatic Arthritis, 2017
Asmita Hazra, Saptarshi Mandal
VE-cadherin is unique in distribution to endothelial cells, and it can act as a master regulator in vasculogenesis. N-cadherins may be found in primitive ECs or in EC mural cell junctions. Unlike other cells where N-cadherin is expressed, in endothelial cells, N-cadherin is often found diffusely all over the EC surface, whereas VE-cadherin is usually found in EC-EC junctions, from where it eventually displaces N-cadherin. VE-cadherin homotypic engagement usually stabilizes vessels and decreases permeability or proliferation. In contrast, N-cadherin usually permits endothelial cell proliferation and motility.
Metastasis
Published in John Melford, Pocket Guide to Cancer, 2017
In adults, the transition of cells from an epithelial type to a mesenchymal type is a normal occurrence, induced in response to wound healing and inflammation. During this process, epithelial cells undergo a series of structural changes that includes, adoption of a mesenchymal phenotype and detachment from one another. These are similar to the observed metastatic changes undergone by transformed epithelial cells in carcinomas. An early event in the transition from an epithelial cell type to a mesenchymal cell type is the cadherin switch. This involves a reduction in levels of E-cadherin, accompanied by an increase in levels of N-cadherin, a protein present in mesenchymal cells. N-cadherin destabilizes the adhesion of epithelial cells, thereby promoting cell separation.
Cell-cell junctions in developing and adult tendons
Published in Tissue Barriers, 2020
Sophia K. Theodossiou, Jett B. Murray, Nathan R. Schiele
N-cadherin is a regulator of cell adhesion and connective tissue morphogenesis that has also been explored in patterning of the musculoskeletal tissues in the limbs. N-cadherin-null mice do not survive in utero unless rescued with transgenic expression of a cardiac cadherin.40 While non-rescued N-cadherin-null mice survive to form forelimb buds at E9.5, they are not viable by E11-E12 due to cardiac malformations, and further limb development cannot be assessed.40 To address this limitation, a follow-up study cultured forelimbs from rescued E10.5 N-cadherin-null mice ex vivo for 7 days (d), and found that the limbs developed and did not differ significantly from wild-type forelimbs in overall morphology, size, and cellular condensation of chondrogenic precursors.41 Although N-cadherin expression was absent in the mutant limbs, expression of cadherin-11 was not affected, indicating that cadherin-11 and other cadherins may drive limb development in the absence of N-cadherin.41
Dynamic Volume Assessment of Hepatocellular Carcinoma in Rat Livers Using a Clinical 3T MRI and Novel Segmentation
Published in Journal of Investigative Surgery, 2018
Lorenzo A. Orci, Graziano Oldani, Stephanie Lacotte, Florence Slits, Iris Friedli, Wolfgang Wirth, Christian Toso, Jean-Paul Vallée, Lindsey A. Crowe
N-cadherin was quantified by Western blot analysis. N-cadherin functions as an invasion promoter and is frequently up-regulated in epithelial tumor cells. It induces changes, rendering the cells more motile and invasive [22]. Cells were plated for 48 hr before lysis for Western blotting. Total protein extracts (40 μg) were loaded on an SDS-PAGE gel. Electrophoresed samples were electro-blotted on polyvinylidene fluoride (PVDF) membranes (Immobilon-P, Sigma Aldrich, Buchs, Switzerland). The membranes were incubated overnight at 4°C with an anti-N-cadherin antibody (32/N-Cadherin, BD Pharmingen, Allschwil, Switzerland) or an anti-beta-catenin (Sigma Aldrich, Buchs, Switzerland). Detection was performed using an anti-rabbit or an anti-mouse Horseradish Peroxidase (HRP)-conjugated secondary antibody (Bio-Rad Laboratories, Cressier, Switzerland).
Sex-dependent expression of N-cadherin-GluA1 pathway-related molecules in the prefrontal cortex mediates anxiety-like behavior in male offspring following prenatal stress
Published in Stress, 2021
Shuya Shao, Jing Li, Shengquan Chen, YanKai Dong, Shang Wang, Zhongliang Zhu, Longshan Xie, Hui Li
N-cadherin plays an important role in the formation of the neural circuit and the development of the nervous system (Yang et al., 2016), such as synapse assembly and synaptic plasticity (Brusés, 2006; Tai et al., 2007). Reduced N-cadherin expression leads to impairment of synaptic function through interactions with GluA1 (Mills et al., 2017), and synaptic deficits in the PFC induce anxiety symptoms (Christoffel et al., 2011; Leuner & Shors, 2013). The third trimester of gestation is a period of accelerated development of synapse formation (Kostović & Jovanov-Milosević, 2006). Thus, PS exposure reduces the expression of N-cadherin, which may impair synaptic formation through interactions with GluA1, resulting in anxiety-like behavior in male offspring.