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A Survey of Newer Gene Probing Techniques
Published in Victor A. Bernstam, Pocket Guide to GENE LEVEL DIAGNOSTICS in Clinical Practice, 2019
Another addition to amplification methods for DNA analysis is the use of a thermostable DNA ligase, which both amplifies DNA and discriminates a single-base substitution. The reaction is composed of two parts — one is a ligase detection reaction (LDR), the product of which is then further amplified in a ligase chain reaction (LCR), in which both strands of genomic DNA are used as targets for oligonucleotide hybridization. Similar to PCR, the ligation products from one round of ligation become templates for another round, and the amount of product exponentially increases when repeated thermal cycling is maintained.
Understanding and Diagnosing Herpes Simplex Virus
Published in Marie Studahl, Paola Cinque, Tomas Bergström, Herpes Simplex Viruses, 2017
A number of NA amplification techniques, such as PCR, ligase chain reaction, strand displacement, transcription mediated amplification, NA sequence based amplification (NASBA), branched DNA technique, and hybrid capture assays, have been described (42).
Target Amplification-Based Techniques
Published in Attila Lorincz, Nucleic Acid Testing for Human Disease, 2016
Antoinette A.T.P. Brink, Peter J.F. Snijders, Chris J.L.M. Meijer
Other cyclic amplification methods such as the ligase chain reaction (LCR) rely on DNA denaturation and renaturation. Some of these methods were developed for specific purposes that could not be covered by a standard PCR.
Tuberculosis in the 21th century: Current status of diagnostic methods
Published in Experimental Lung Research, 2018
Patrícia Poeta, Vanessa Silva, Andreia Guedes, José Eduardo Pereira, Ana Cláudia Coelho, Gilberto Igrejas
The Nucleic Acids Amplification Tests (NAAT) is a method that can be used to detect MTB directly from clinical specimens, however, it has not been endorsed yet by the WHO for use in low- to middle-income countries.42 NAAT consists of the amplification of nucleic acids, DNA and RNA, and sometimes these tests can be combined with very specific detection systems and in particular with the hybridization of different probes.20 NAAT include different methodologies such as PCR, real-time PCR (RT-PCR), Ligase Chain Reaction (LCR), DNA fingerprinting, Line Probe Assays (LPAs), Xpert MTB/RIF tests, Spoligotyping and Loop-mediated isothermal amplification. The NAAT are tests which make a rapid diagnosis of TB possible. However, these tests show variable sensitivity taking into account the sample and its bacterial load. The NAAT require expensive equipment and skilled laboratory and trained technicians in these methodologies.26