Glycosylamine

Glycosylamine

Cyanogenic Glycosides

Published in Dongyou Liu, Handbook of Foodborne Diseases, 2018

The linkage between the glycone and aglycone groups in glycoside may be an O- (O-glycoside), N- (glycosylamine), S- (a thioglycoside), or C- (C-glycoside) glycosidic bond. In addition, depending on whether the glycosidic bond lies “below” or “above” the plane of the cyclic sugar molecule, glycoside may be referred to as α-glycosides (hydrolyzed by α-amylase) or β-glycosides (hydrolyzed by β-glucosidase or emulsin).

Novel methods in glycomics: a 2019 update

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Journal Information

Published in Expert Review of Proteomics, 2020

Wei-Qian Cao, Ming-Qi Liu, Si-Yuan Kong, Meng-Xi Wu, Zheng-Ze Huang, Peng-Yuan Yang

Glycan reducing end 18O labeling (GREOL) was first reported by releasing glycans with Endo H in heavy water, resulting in a 2 Da mass difference between 18O-labeled glycans and unlabeled glycans [203]. A method named PCGOL, in which PNGase F releases a glycosylamine that undergoes conversion in 18O-water to labeled glycan, was further developed [204]. Additionally, the same group developed the glycan reducing end dual isotopic labeling (GREDIL) method, which utilized NaBH4/NaBD4 to increase the mass difference of 16O/18O-labeled glycans to 3 Da [205]. Enzymatic labeling methods are easy to control and suitable for a variety of samples.

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Cyanogenic Glycosides

Novel methods in glycomics: a 2019 update