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The Bioenergetics of Mammalian Sperm Motility
Published in Claude Gagnon, Controls of Sperm Motility, 2020
Storey has proposed that the metabolic capacity of sperm mitochondria reflects the availability of substrates in the lumen of the oviduct. Thus, rabbit sperm mitochondria (or more strictly hypotonically treated rabbit sperm) can oxidize lactate very readily and lactate is present at a concentration of up to 3 mM in rabbit oviduct fluid (Table 1). However, they lack L-glycerol 3-phosphate oxidase, glutamate oxaloacetate transaminase, and acyl CoA-carnitine transferase, and consequently are unable to utilize endogenous lipids and phospholipids. Bovine oviduct fluid contains a low concentration of oxidizable substrate, but bull sperm can rapidly metabolize endogenous lipids.54,55
Recent trends and perspectives in enzyme based biosensor development for the screening of triglycerides: a comprehensive review
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2018
Vinita Hooda, Anjum Gahlaut, Ashish Gothwal, Vikas Hooda
Dissolved oxygen (DO) which is consumed in the oxidation reaction of triglycerides by membrane bound with enzymes lipase, glycerol kinase (GK), glycerol-3-phosphate oxidase (GPO) is measured in these biosensors. This dissolved O2 is directly proportional to the concentration of TG. These biosensors are very simple to handle and can be easily operated in the laboratories without employment of special trainee and expertise. The disadvantage with DO based TG biosensors is that atmospheric oxygen can interfere in their working and thus resulting in low sensitivity. Earlier in 1984, Kelly et al. developed a TG measurement system, which involved indirect monitoring of NADH using its reaction with oxygen by horseradish peroxidase (HRP) [32]. The optimal response was shown at 33˚C temperature and 7.5pH within 300 s by the proposed biosensor. The biosensor showed linearity and LOD as 0.05 to 0.3 mM and 0.05 mM, respectively. Table 2 provides a comparison of various DO metric based TG biosensors [33,34].
The Effect of Phytosterol-Rich Fraction from Palm Fatty Acid Distillate on Blood Serum Lipid Profile of Dyslipidemia Rats
Published in Journal of Dietary Supplements, 2018
Kgs Ahmadi, Huda Oktafa, Teti Estiasih
The principle of triglyceride analysis in the sample is that triglycerides are hydrolyzed by lipase into glycerol and free fatty acids. Glycerol is then phosphorylated by glycerol kinase in the presence of ATP and Mg+2 ions. Glycerol-3-P is oxidized by glycerol-3-phosphate oxidase (GPO) in the presence of oxygen into dihydroxyacetone phosphate and hydrogen peroxide. A colored product is produced after reaction of hydrogen peroxide with 4-aminoantipyrine and phenol-derivative in the presence of the peroxidase. Absorbance was measured at 500 nm (Bucolo and David, 1973).
Co-drug of isoniazid and sulfur containing antioxidant for attenuation of hepatotoxicity and treatment of tuberculosis
Published in Drug and Chemical Toxicology, 2022
Neha V. Bhilare, Suneela S. Dhaneshwar, Kakasaheb R. Mahadik, Arunava Dasgupta
Commercially available spectrophotometric kits purchased from Coral Clinical Systems, Mumbai, Maharashtra were used for the estimation of triglyceride (TG) and cholesterol content. For measuring TG in plasma, a colorimetric (GPO/PAP) method was employed based on the glycerol-3-phosphate-oxidase enzyme. Estimation of cholesterol was carried out using CHOD/PAP method (Sullivan et al. 1985)