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What Do the Heart Arteries Do When They Are Damaged? The Infinite Insults and Finite Responses in CHD
Published in Mark C Houston, The Truth About Heart Disease, 2023
A number of tests exist to measure the total antioxidant capacity of the blood. One of the most common total antioxidant capacity assays is the Trolox equivalent antioxidant capacity assay (TEAC). The oxygen radical antioxidant capacity (ORAC) assay and ferric reducing antioxidant power (FRAP) are other common oxidative stress tests.
From Designer Food Formulation to Oxidative Stress Mitigation: Health-Boosting Constituents of Cabbage
Published in Megh R. Goyal, Hafiz Ansar Rasul Suleria, Ramasamy Harikrishnan, The Role of Phytoconstitutents in Health Care, 2020
Faiza Ashfaq, Masood Sadiq Butt, Ahmad Bilal, Kanza Aziz Awan, Hafiz Ansar Rasul Suleria
The TEAC of white cabbage was measured as 1.73 µM Trolox/g F.W. [15]. Furthermore, the TEAC of green and red cabbage were 4.92 and 13.77 µM Trolox/g F.W., respectively [125]. Podsedek et al., [119] measured the ABTS values for white and red cabbage as 1.34 to 1.81 and 9.81 to 12.64 µM TEAC/g F.W., respectively.
Antioxidant assays
Published in Roger L. McMullen, Antioxidants and the Skin, 2018
Throughout the years, the ABTS assay has undergone various revisions.19–23 However, the form of the assay that has prevailed is often referred to as a decolorization assay.24 this is because the radical, ABTS•+, absorbs visible light (Figure 6.7); however, as ABTS•+ reacts with the antioxidant of interest the reduced form (ABTS), which has negligible absorbance in the visible region, is produced. Often, this method is referred to as the aforementioned TEAC because the results obtained for the antioxidant of interest are compared to results obtained when the water-soluble vitamin E derivative, Trolox, is utilized in this assay.
Effects of lipid formulations on clove extract spray dried powders: comparison of physicochemical properties, storage stability and in vitro intestinal permeation
Published in Pharmaceutical Development and Technology, 2018
Diego F. Cortes-Rojas, Claudia R. F. Souza, Mong-Jen Chen, Guenther Hochhaus, Wanderley P. Oliveira
Simple in vitro methods involving free radicals are commonly employed for the screening of natural antioxidants. One of the most employed methods is the ABTS that was carried out as described by Re et al. (Re et al. 1999). The ABTS radical cation was generated by the reaction of an ABTS 7 mM solution and a potassium persulfate 2.45 mM solution at room temperature in the dark for 16 h. This radical solution was diluted with ethanol to reach an absorbance of 0.700 ± 0.020 at 734 nm. The samples were prepared by dissolving the powders in water first and then in absolute ethanol, testing each sample in three different concentrations. 30 μL of the dilution were mixed with 3 mL of the ABTS radical solution and after 6 min of reaction, the absorbance was recorded at 734 nm in a UV–VIS HP 8453 spectrophotometer (Agilent Technologies, Waldbronn, Germany) using absolute ethanol as blank. The results were expressed in terms of TEAC employing an analytic curve of Trolox in concentrations from 0 to 19.8 μM (Re et al. 1999).
Profiling donepezil template into multipotent hybrids with antioxidant properties
Published in Journal of Enzyme Inhibition and Medicinal Chemistry, 2018
Eva Mezeiova, Katarina Spilovska, Eugenie Nepovimova, Lukas Gorecki, Ondrej Soukup, Rafael Dolezal, David Malinak, Jana Janockova, Daniel Jun, Kamil Kuca, Jan Korabecny
TEAC assay is a simple and convenient method for determination of TAC based on the ability of antioxidants to scavenge the stable ABTS radical cation (a blue–green chromophore with an absorption maximum wavelength at 734 nm). Potential antioxidants can neutralize the ABTS radical cation by either radical quenching through hydrogen atom donation or by direct reduction through electron donation. Thereby, the antioxidants decolorize ABTS radical cation and spectrophotometrically can be measured a decrease in absorbance (the loss of its colour). This depends on the intrinsic antioxidant activity, concentration sample and also reaction duration35,44,46. Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxyl acid, water soluble analogue of vitamin E) can be used as standard antioxidant and results of the experiments are usually expressed as Trolox equivalent40. This assay is usually classified as ET-based method.
Myricetin derivative-rich fraction from Syzygium malaccense prevents high-fat diet-induced obesity, glucose intolerance and oxidative stress in C57BL/6J mice
Published in Archives of Physiology and Biochemistry, 2023
Devi Nallappan, Kien Chai Ong, Uma Devi Palanisamy, Kek Heng Chua, Umah Rani Kuppusamy
TEAC level in tissue homogenates was determined according to the method outlined by Re et al. (1999). The 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical reagent was prepared by reacting 7 mM ABTS with 2.45 mM potassium persulphate. The reagent was incubated in the dark at room temperature for 12–16 h to produce ABTS radicals. The ABTS radical solution was then diluted with absolute ethanol to an absorbance of 0.68 to 0.72 at 734 nm. The sample (10 μL) was then added to 100 μL of appropriately diluted ABTS radical solution. The absorbance was measured after 6 min against blank (PBS) using a microplate reader. Trolox (water-soluble Vitamin E analogue) was used as a standard, and the result was expressed as µmol Trolox equivalent/g tissue.