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Plants from Brazil Used Against Snake Bites
Published in Mahendra Rai, Shandesh Bhattarai, Chistiane M. Feitosa, Wild Plants, 2020
Jocimar de Souza, Bruna Stramandinoli Deamatis, Fernanda Mayumi Ishii, Ingrid Francine Araújo de Oliveira, Gustavo Rodrigues Toledo Piza, Jorge Amaral Filho, Edson Hideaki Yoshida, José Carlos Cogo, Angela Faustino Jozala, Denise Grotto, Rauldenis Almeida Fonseca Santos, Yoko Oshima-Franco
It is worth pointing out, according to Alam et al. (2013) “among in vitro free radical scavenging methods, DPPH method is furthermore rapid, simple (i.e., not involved with many steps and reagents), and inexpensive in comparison to other test models”. Besides, regarding ex vivo antioxidant evaluation, this is the first study using the nutrient solution (Tyrode) as sample for bathing the phrenic-diaphragm nerve preparation, which justifies the importance of the study.
Assessment of Secondary Metabolites in the Yams of Dioscorea oppositifolia L. & Dioscorea pentaphylla L.
Published in Parimelazhagan Thangaraj, Phytomedicine, 2020
S. Vivek, Y. Aron Santhosh Kumar, M. Palanisamy
The DPPH is a stable free radical, which has been widely accepted as a tool for estimating free radical scavenging activities of anti-oxidants. The DPPH is a stable free radical and accepts an electron or hydrogen radical to become a stable diamagnetic molecule (Kalaivani and Mathew 2010). The degree of discoloration indicates the potential of the plant extract to scavenge a free radical due to its ability to donate a hydrogen proton. The concentration-dependent curve of the DPPH radical scavenging activity of D. oppositifolia and D. pentaphylla compared well with the ascorbic acid. The result is indicative of the hydrogen donating ability of D. pentaphylla, since the effects of anti-oxidants on the DPPH radical scavenging is thought to be due to their hydrogen donating ability (Conforti et al. 2005).
Advanced Glycation End Products—A Special Hazard in Diabetes
Published in Robert Fried, Richard M. Carlton, Type 2 Diabetes, 2018
Robert Fried, Richard M. Carlton
Traditional preparations and the ethanolic extracts of E. tinifolia, M. zapota, O. campechianum, and P. auritum showed significant activity in the DPPH reduction assay. Results suggest that the metabolites responsible for the detected radical-scavenging activity are different from those involved in inhibiting AGE formation (Dzib-Guerra, Escalante-Erosa, García-Sosa et al. 2016). DPPH is a common antioxidant assay.
The Radioprotective Effect of Ascorbic Acid and Kefir against Genotoxicity Induced by Exposure in Mice Blood Lymphocytes
Published in Nutrition and Cancer, 2021
Farideh Koohian, Daryoush Shahbazi-Gahrouei, Mahbobeh Koohiyan, Ahmad Shanei
DPPH is a stable organic free radical and is a known proton-radical scavenging effect indicating the power of antioxidants to gift hydrogen or electrons. This method is widely used owning to it is simple, rapid, sensitive and reproducible. Decrease in the concentration of the DPPH solution in the presence of an antioxidant is permitted to evaluate the reduction in its absorbance at a particular wavelength (14). The Free radical-scavenging activity of ascorbic acid and kefir measured as blenching of 1,1-diphenyl-2-picryl hydrazyl radical (DPPH), butylated hydroxytoluene (BHT) used as an antioxidant reference (15). Different concentrations of sample solution (0.02-0.4 mg/mL) were added to an equal volume of a methanol solution of DPPH (100 mM). After one quarter hour, the absorbance of these solutions was assessed spectrophotometrically at 517 nm against the black sample. The measurement was repeated with three sets. The percentage of the inhibition of DPPH free radical was calculated using the equation:
Synthesis of silver nanoparticles using Ziziphus nummularia leaf extract and evaluation of their antimicrobial, antioxidant, cytotoxic and genotoxic potential (4-in-1 system)
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2021
Hemali Padalia, Sumitra Chanda
DPPH activity was found to increase in a dose-dependent manner. The IC50 value of AgNPs was 520 µg/mL (Figure 7(A)). Kanipandian et al.[17] reported DPPH free radical scavenging activity of AgNPs synthesized from Cleistanthus collinus, 69% inhibition at a concentration of 1000 µg/mL however the activity was quite less as compared to the present results; the synthesized AgNPs showed 88% inhibition at concentration of 960 µg/mL. ABTS radical cation scavenging activity was also dose dependent, IC50 value of synthesized AgNPs was 55 µg/mL (Figure 7(B)).Nagajyothi et al., [26] also reported dose-dependent ABTS radical cation scavenging activity for AgNPs synthesized using Inonotus obliquus extract. The reducing capacity of AgNPs showed a steady increase in the absorbance of the reaction mixture with increase in concentration of AgNPs (Figure 7(C)). Similar results are reported by [5] for AgNPs synthesized using Syzygium cumini seed extract.
Development and optimization of sitagliptin and dapagliflozin loaded oral self-nanoemulsifying formulation against type 2 diabetes mellitus
Published in Drug Delivery, 2021
Mohsin Kazi, Abdulmohsen Alqahtani, Ajaz Ahmad, Omar M. Noman, Mohammed S. Aldughaim, Ali S. Alqahtani, Fars K. Alanazi
For antioxidant activity, DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging assay was employed. This method was carried out as previously described by Brand-Williams et al. (1995). The method quantifies free radical scavenging capacity of the investigated samples. DPPH is a molecule that contains a stable free radical. In the presence of an antioxidant which is able to donate an electron to DPPH, the change in the absorbance at 517 nm is followed spectrophotometrically (UV mini-1240, Shimadzu, Japan). Various concentrations of DPPH (10, 50, 100, 500, and 1000 μg/mL) were used. The assay mixture was made up of a total volume of 1 mL, containing 500 μL of the sample, 125 μL of the prepared DPPH, and 375 μL of methanol. As positive control, ascorbic acid was used. The samples were incubated for 30 min at 25 °C, after which the decrease in absorbance was measured at λ = 517 nm. Radical scavenging activity of the samples were calculated using the following equation below: