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Role of Histone Methyltransferase in Breast Cancer
Published in Meenu Gupta, Rachna Jain, Arun Solanki, Fadi Al-Turjman, Cancer Prediction for Industrial IoT 4.0: A Machine Learning Perspective, 2021
Surekha Manhas, Zaved Ahmed Khan
Methylation at H3K36 histone protein residue is recognized as an abundant mark of histone that is highly conserved in eukaryotes. In addition, mono/di/trimethylation of H3K36 residue of H3 histone exists in yeast, and all these states of H3K36 are catalyzed through SET2. In the other cases, mammals have all other H3K36 methylation multiple writers, which include SETD2, NSD (1–3) family, SMYD2, ASH1L, and SETMAR, but SETD2 is recognized as the main sole enzyme that is responsible for proceeding the process of trimethylation at H3K36 in vivo [55]). Captivatingly, uncoupling of the activity H3K36me3 from H3K36me (1–2) over evolutionary alludes towards the biologically specified variable roles associated with each state of methylation. In addition, H3K36me3 is mostly associated with the active transcribed specific regions of specific genes and H3K36me3 level increase in the direction of the 30th end of active genes [10].
Aging Epigenetics
Published in Shamim I. Ahmad, Aging: Exploring a Complex Phenomenon, 2017
Vasily V. Ashapkin, Lyudmila I. Kutueva, Boris F. Vanyushin
In yeast, H3K36me3 methyltransferase mutants (set2Δ) were found to have shortened life spans, whereas the H3K36me3 demethylase mutants (rph1Δ) increased life spans [54]. These effects were correlated with changes in transcription fidelity. An increase in cryptic transcription from intragenic promoter-like sequences was observed in old yeast cells, whereas H3K36me3 demethylase mutation suppressed such cryptic transcription. Both age-dependent cryptic transcription and H3K36me3 loss were most obvious in the same set of long and infrequently transcribed genes, and both were suppressed by rph1Δ mutation. An age-dependent increase in cryptic transcription was also observed in a large (>400) set of C. elegans genes. Thus, a decrease in transcription fidelity may be an evolutionarily conserved aging mechanism.
Staphylococcus
Published in Dongyou Liu, Laboratory Models for Foodborne Infections, 2017
Mar Rodríguez, Alicia Rodríguez, María Jesús Andrade, Elena Bermúdez, Juan José Córdoba
Immunological tools are the most commonly used methods for detecting SEs in foods. Commercially available kits have been developed according to two different principles: (1) enzyme immunoassay comprising ELISA (TECRA Kit, TRANSIA PLATE, and RIDASCREEN SET) and enzyme-linked fluorescent assay (ELFA) (VIDAS SET and VIDASTM SET2), and (2) latex agglutination (SET-RPLA). It is widely recognized that the use of immunological methods to detect contaminants in food matrices is a difficult task, mainly because of the lack of specificity and sensitivity of the marketed kits. Moreover, only antibodies against SEA to SEE, SEG, SEH, and SElQ were available until recently. The immunological tools do not detect the other SEs.25 The main drawback of current methods to detect enterotoxins based on specific polyclonal or monoclonal antibodies remains their high cost.43
The metabolic enzyme arginase-2 is a potential target for novel immune modulatory vaccines
Published in OncoImmunology, 2020
Stine Emilie Weis-Banke, Mie Linder Hübbe, Morten Orebo Holmström, Mia Aaboe Jørgensen, Simone Kloch Bendtsen, Evelina Martinenaite, Marco Carretta, Inge Marie Svane, Niels Ødum, Ayako Wakatsuki Pedersen, Özcan Met, Daniel Hargbøl Madsen, Mads Hald Andersen
THP-1 were cultured in RPMI (Gibco) supplemented with 10% FBS. Set2 cells were cultured in RPMI with 20% FBS. OCI-AML-2 cells were cultured in Alpha-MEM (Life Technologies) with 10% FBS. MONO-MAC-1 cells were cultured in RPMI supplemented with 10% FBS, 1 mM sodium pyruvate (Life Technologies), 2 mM L-glutamine (Life Technologies) and 1x non-essential amino acids (Life Technologies). All cell lines were tested and confirmed negative for mycoplasma. Cells were passaged 2–3 times a week. Cytokine stimulation with IL-4 (400 U/ml), IL-13 (50 ng/ml), IFNy (100 U/ml) or cytokine cocktail (400 U/ml IL-4, 1000 U/ml GM-CSF and 1000 U/ml TNFα) was done by seeding of 0.5–0.75 mio cells pr. ml medium supplemented with the respective cytokines and 48 hrs of incubation before cells were harvested for various experiments. All cytokines are from Trichem.
Treating donor cells with 2-PCPA corrects aberrant histone H3K4 dimethylation and improves cloned goat embryo development
Published in Systems Biology in Reproductive Medicine, 2018
Tingchao Mao, Chengquan Han, Ruizhi Deng, Biao Wei, Peng Meng, Yan Luo, Yong Zhang
During embryonic development, H3K4 dimethylation (H3K4me2) is mainly involved in the transcriptional activation of some genes (Santosrosa et al. 2002), playing important roles in embryonic development and cell differentiation. Situated in the gene promoter region, H3K4me2 is an epigenetic marker related to transcriptional activation that is mediated by H3K4 methyltransferases, including Set1 and Set2 (Kim and Buratowski 2009). Demethylation of H3K4me2 is implemented mainly with the assistance of lysine acid-specific demethylase 1 (LSD1), which through its action inhibits gene expression (Metzger et al. 2005).