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Vinca rosea (Madagascar Periwinkle) and Adhatoda vesica (Malabar Nut)
Published in Azamal Husen, Herbs, Shrubs, and Trees of Potential Medicinal Benefits, 2022
Rajib Hossain, Md Shahazul Islam, Dipta Dey, Muhammad Torequl Islam
The phytochemical content and antioxidant activity of A. vasica leaves, total antioxidant activity, 2, 2 diphenyl-1-picrylhydrazyl radical-scavenging activity, reducing power potential, and iron-chelating activity were used to determine the antioxidant activity of A. vasica methanol extract. The agar well diffusion technique was used to test antimicrobial activity. Total phenolic content was determined using the Folin-Ciocalteu reagent technique, and total flavonoid content was determined using the aluminum chloride method. Saponins, oils and lipids, phytosterol, phenolic compounds, tannins, glucose, alkaloids, flavonoids, and proteins were discovered in the leaves of A. vasica. Various antioxidant tests revealed that the extract has a strong antioxidant activity. The presence of significant quantities of polyphenolic substances (phenolic compounds and flavonoids) in the extract of A. vasica may be the cause of the plant's antioxidant action. Furthermore, the extract exhibited moderate antibacterial and cytotoxic action (lethality of brine shrimp) (Kotakadi et al., 2013).
Total polyphenol and flavonoid content comparation of Kertasari Arabica coffee (coffea arabica L.) leaves, pulp, and beans
Published in Ade Gafar Abdullah, Isma Widiaty, Cep Ubad Abdullah, Medical Technology and Environmental Health, 2020
E.R. Sadiyah, L. Purwanti, S. Hazar, S.O. Sasmita, A. Yuniarti
Folin-Ciocalteu reagent was used to measure the total polyphenol content in the samples. This method is based on hidroxy phenolic residue oxidation by the reagent in base condition that would produce a blue molybdenum-tungstate complex (Zhang & Hamauzu 2004). According to the calculation results, the level of total polyphenol content in leaves, peels, and beans of Kertasari Arabica coffee were 180.98, 37.13, and 112.31 mg GAE/g extract, respectively.
Method of Fractionating Subcellular Particles to Determine Intracellular Localization of Radiotracers
Published in Lelio G. Colombetti, Principles of Radiopharmacology, 2019
H. Orii, K. Samezime, K. Komine
Protein: A modification of the method of Lowry was used. An equal volume of 1% cuprous sulfate and 0.02% sodium potassium tartrate was mixed with 50 volumes of 2% sodium potassium tartrate was mixed with 50 volumes of 2% sodium carbonate in 0.1 N-sodium hydroxide (Folin-Ciocalteu reagent). An amount of 2.5 mℓ of twofold diluted Folin-Ciocalteu reagent was added to 0.2 mℓ sample, mixed, and the absorbance at 660 nm was measured after 20 min. The standard curve was plotted on albumin.
Antioxidant and cytoprotective effects of sequentially extracted Terminalia prunioides pods
Published in Egyptian Journal of Basic and Applied Sciences, 2023
Phazha Baeti, Keagile Bati, Kabo Masisi, Goabaone Gaobotse, Tebogo Kwape
The TPC of Terminalia prunioides pods were determined using a method by [25]. 200 µL of gallic acid at concentrations 0.0312, 0.0625, 0.125, 0.25, 0.5 and 1 mg/mL, 2 mL distilled water and 200 µL of 10% Folin-ciocalteu reagent were mixed and incubated for 8 minutes in the dark. 600 µL of 20% sodium carbonate was added and left to incubate in the dark for 2 hours at room temperature. Absorbance was read at 760 nm using a Spec200E UV-V, and the results for gallic acid were used to plot a standard curve that was used in calculating TPC of extracts. 200 µL of 1 mg/mL extracts, 2 mL distilled water and 200 µL of 10% Folin-ciocalteu reagent, were mixed and incubated for 8 minutes in the dark. 600 µL of 20% sodium carbonate was added and left to incubate in the dark for 2 hours at room at temperature. Absorbance was read at 760 nm using a Spec200E UV-V (Thermofisher Scientific, United States of America). Gallic acid standard curve (R2 = 0.99) was used in determining TPC. The TPC was calculated as,
Copper oxide (CuO) and manganese oxide (MnO) nanoparticles induced biomass accumulation, antioxidants biosynthesis and abiotic elicitation of bioactive compounds in callus cultures of Ocimum basilicum (Thai basil)
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2021
Saher Nazir, Hasnain Jan, Gouhar Zaman, Taimoor Khan, Hajra Ashraf, Bisma Meer, Muhammad Zia, Samantha Drouet, Christophe Hano, Bilal Haider Abbasi
For the determination of total phenolic contents, the protocols of Slinkard and Singleton (1977) were used with slight changes [34]. Concisely, 20 μL of the sample, 90 μL of Folin-Ciocalteu reagent and 90 μL of sodium carbonate were mixed. The reaction mixture was incubated for 1.5 h at room temperature and 200 ul was then added into microplate wells (96 well plates). The absorbance of reaction mixture was measured at 630 nm with UV/VIS-DAD spectrophotometer (Halo DR-20, UV-VIS spectrophotometer, Dynamica Ltd, Victoria, Australia). Positive and negative controls were used by employing gallic acid (1 mg/mL) and methanol (20 μL), respectively. The calibration curve (0–50 μg/mL, R2 = 0.968) was plotted by using gallic acid as standard and the TPC was expressed as gallic acid equivalents (GAE)/g of DW. Total phenolic production (TPP) was measured by using the following formula and estimated in mg gallic acid/L.
Gastroprotective effect of leaf extract of two varieties grapevine (Vitis vinifera L.) native wild and cultivar grown in North of Tunisia against the oxidative stress induced by ethanol in rats
Published in Biomarkers, 2020
Nabil Saadaoui, Asma Weslati, Taha Barkaoui, Ikram Khemiri, Wafa Gadacha, Abdelaziz Souli, Moncef Mokni, Mounira Harbi, Mossadok Ben-Attia
The Total phenolic content (TPC) of grapevine leaves extracts was measured as described by Jayaprakasha et al. (2003). 200 μL of samples were assayed with 1.0 mL of the Folin–Ciocalteu reagent (diluted in 1:10 ratio). The mixture was shaken and stabilized for 3 min before adding 800 µL of sodium carbonate (Na2CO3, 7%). Samples and blank were thoroughly agitated and vortexed. After that the obtained solution was maintained in the dark for 90 min and the absorbance was measured at 760 nm. Total o-diphenols (ToPC) was determined by molybdate assay according to the method of Mnari et al. (2016). One mL of a solution of HCl (0.5 N), 1 mL of a solution of a mixture of NaNO2 (10 g) and NaMoO4·2H2O (10 g) in 100 mL H2O, and finally 1 mL of a solution of NaOH (1 N) were added to 100 µL of the extract. After incubation for 30 min at room temperature, the absorbance was read at 500 nm. Gallic acid (from 0.01 to 0.1 mg/mL by 0.01 mg/mL steps) was used for calibration and TPC were expressed as milligrams of gallic acid equivalents per gram of dry matter (mg GAE/g DM). UV-visible analyses were carried out with the BioRad SmartSpec™ 3000 Scan UV-visible spectrophotometer.