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Breast Imaging with Radiolabeled Peptides
Published in Raymond Taillefer, Iraj Khalkhali, Alan D. Waxman, Hans J. Biersack, Radionuclide Imaging of the Breast, 2021
Eric P. Krenning, Marion de Jong, Roelf Valkema, Casper H.J. van Eijck
The Na-diethyleletriamepenta-acetic acid (DTPA, Fluka) derivative of octreotide was synthesized by Novartis (Basel, Switzerland) using the protected [e-t-buty-loxy-carbonyl-Lys5]octreotide as starting material which was available by the reaction of octreotide with di-t-butyl-dicarbonate [(Boc)20, Fluka] in dimethylformamide. DTPA was coupled to the selectively protected octreotide in form of its dianhydride. Purification of the product was achieved by silica gel chromatography in order to separate the wanted [DTPA°-e-Boc-Lys5]octreotide from the contaminating double-substituted DTPA-derivative and unreacted starting material. Deprotecting with trifluoracetic acid and subsequent sequential purificationyielded homogeneous [DTPA°]octreotide (also called [DTPA-D-Phe']octreotide) as lyophilisate. The purity was checked by reverse-phase HPLC. Structure and amino acid composition were proven by means of nuclear magnetic resonance, fast atom bombardment mass spectrometry, and amino acid analysis [49],
A clinical and in-silico study exploring the association of CASP-3, NF-kB, miR-187, and miR-146 in pre-eclampsia
Published in Hypertension in Pregnancy, 2021
Charu Sharma, Purvi Purohit, Manoj Khokhar, Anupama Modi, Pratibha Singh, Shashank Shekhar, Shailja Sharma, Meenakshi Gothwal, Praveen Sharma
We used TRIzol (HiMedia RNA-XPressTM Reagent), a commercially available RNA extraction reagent, for extracting total RNA from the (MPT and FPT) samples. The MPT and FPT were collected from each case and control just after birth by the operating gynecologist and were immediately transferred in 1% phosphate buffer saline (PBS) in a sterile container. To 100 mg of MPT and FPT, 350 μL TRIzol was added for homogenization. Similarly, RNA from whole blood samples of the study population was extracted using RBC lysis buffer in a ratio of 1:3. The sample was centrifuged at 1300 rpm at 4°C for 15 minutes, and 1 ml PBS was added to dissolve the pellet, followed by centrifugation at 3000 rpm for 2 minutes. TRIzol was added to the pellet (in a ratio of 1000:1) to obtain a homogeneous solution. Subsequently, a similar protocol for RNA isolation for tissue and blood was carried out. As follows, an equal amount of chloroform was added for phase separation and after then isopropanol was used to remove the aqueous layer (in a ratio of 1:1), and the contents were briefly vortexed. The pellet was then dissolved in 1 ml of 75% ethanol and centrifuged at 12,000 rpm for 5 mins. Twenty microliters of diethyl dicarbonate (DEPC) treated water was added after air-drying the pellet, followed by incubation at 65°C for 3–4 minutes. The total RNA was quantified using a microplate reader (BioTek Instruments, Inc.). Only those RNA samples with a 260/280 and 260/230 ratio of ≥1.8 were considered as suitable for RT-PCR.
Lysine-mediated hydroxyethyl starch-10-hydroxy camptothecin micelles for the treatment of liver cancer
Published in Drug Delivery, 2020
Guofei Li, Mingming Zhao, Limei Zhao
10-Hydroxy camptothecin (99%) was purchased from Wuhan Lishizhen Pharmaceutical Co., Ltd. (Wuhan, China). 10-Hydroxy camptothecin injection (1 mg/mL) was purchased from Shengjing Hospital of China Medical University (Shenyang, China). Hydroxyethyl starch (HES, 130 kDa/0.4) was obtained from Chongqing Daxin Pharmaceutical Co., Ltd. (Chongqing, China). The Mw of HES is 130 kDa and the degree of substitution is 0.4. 2,2′-dithiodipyridine (99%), dithiothreitol (98%), 3-mercaptopropionic acid (98%), N,N′-dicyclohexycarbodiimide (99%), 4-dimethylaminopyridine (99%) and N-hydroxysuccinimide (99%), N-(tert-butoxycarbonyl)glycine (98%), succinic anhydride (99%), di-tert-butyl dicarbonate (99%) were purchased from Shanghai Darui Fine Chemical Co., Ltd. (Shanghai, China). 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide and human liver cancer Hep-G2 cell line were purchased from Shanghai Institutes for Biological Sciences (Shanghai, China).
Preparation and evaluation of tumour microenvironment response multistage nanoparticles for epirubicin delivery and deep tumour penetration
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2018
Jialing Dai, Shangcong Han, Fang Ju, Mei Han, Lisa Xu, Ruoyu Zhang, Yong Sun
tBAM was synthesized according previous procedure [19]. Ethanolamine was dissolved in 100 ml, 1 M, NaOH, and stirred. Then, di-tert-butyl dicarbonate (Boc2O) was dissolved in 50 ml 1,4-diethylene dioxide added into ethanolamine solution, reaction 48 h. Termination reaction with water addition and extracted with ethyl acetate three times. The organic phase was washed with saturated salt water and dried with anhydrous magnesium sulphate. After vacuum drying, a yellow oil product, Boc-ethanolamine, was obtained. Boc-ethanolamine and triethylamine were dissolved in anhydrous CH2Cl2, pre cooling in an ice water bath for 20 min and nitrogen protection, under magnetic stirring condition by dropwise addition of methacryloyl chloride and sealing. The reaction mixture was allowed to warm to room temperature and stirred overnight. The reaction mixture was washed with water, 10% citric acid and 10% K2CO3, sat.NaHCO3 and brine. The organic layer was dried over Na2SO4, and the solvent was evaporated under reduced pressure. The product was purified by recrystallization from CH2Cl2/hexane to give the product.