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Pseudomonas
Published in Dongyou Liu, Handbook of Foodborne Diseases, 2018
Rajasekharan Sharika, Krishnaswamy Balamurugan
The classical and standard method for the detection of Pseudomonas is through a culture-dependent noninvasive method of oropharyngeal swab and sputum analysis, in contrast to bronchoalveolar lavage fluid (BALF) aspiration, which is an invasive technique to sample the lower respiratory tract. Samples are further cultured in Pseudomonas isolation agar or cetrimide agar. Polymerase chain reaction based diagnosis methods targeting outer membrane protein (oprL) and exotoxin A (ETA) genes allow early detection of pathogen, with around 93% sensitivity.117 Antibody detection method through enzyme-linked immunosorbent assay for phospholipase C, exotoxin A, alkaline protease, and elastase is also a reliable method for diagnosis.118
Aeromonas hydrophila biofilm, exoprotease, and quorum sensing responses to co-cultivation with diverse foodborne pathogens and food spoilage bacteria on crab surfaces
Published in Biofouling, 2018
Iqbal Kabir Jahid, Md. Furkanur Rahaman Mizan, Jinjong Myoung, Sang-Do Ha
Preliminary experiments were performed to identify the appropriate selective medium for isolating bacteria from dual-cultures. A. hydrophila was selected using thiosulfate-citrate-bile salts-sucrose agar (TCBS) (Oxoid, Hampshire, UK). S. Typhimurium was selected using brilliant green agar (BGA) containing nalidixic acid (20 mg ml−1) and novobiocin (25 mg ml−1) (Jahid et al. 2014). L. monocytogenes was selected from monoculture and dual culture with A. hydrophila using PALCAM selective media (Oxoid). P. aeruginosa and P. fluorescens were grown in cetrimide agar and Pseudomonas Agar, respectively. P. carotovorum grown on TSA agar was separated from A. hydrophila based on colony morphology; on TSA agar, A. hydrophila forms opaque colonies, whereas P. carotovorum forms transparent colonies and can be further confirmed by growth on TCBS agar plates. Negative growth on TCBS revealed that the strains were P. carotovorum. PALCAM agar and cetrimide agar plates were incubated at 37 °C for 48 h. TSA, TCBS, BGA, and Pseudomonas Agar plates were incubated at 30 °C for 24 h. The Vibrio harveyi was initially grown in LB medium and luminescence was determined using autoinducer bioassay (AB) medium (Bassler et al. 1993). For the violacein assay, CV026 was grown in LB medium without NaCl.
Dental unit water content and antibiotic resistance of Pseudomonas aeruginosa and Pseudomonas species: a case study
Published in Journal of Oral Microbiology, 2022
M. Tesauro, M. Consonni, I. Grappasonni, G. Lodi, R. Mattina
Colonies were selected on the basis of their morphology and colorcolor, and 286 of them were isolated from Cetrimide agar; subsequently, 14 strains were eliminated because of contamination with molds. In assessing the main biochemical properties of the remaining 272 strains, 163 strains were positive and 109 negative for the fluorescence tests, and 238 positive and 34 negative for oxidase, respectively. Therefore, DNA was extracted from 272 strains and qualitative PCR for P. aeruginosa was applied showing 70/272 (25.7%) positive samples; after the elimination of dubious samples, 198 were tested by PCR for identifying Pseudomonas spp., showing 105/198 (53.3%) positive strains (Figure 1).
Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced by Pseudomonas aeruginosa isolates of pus samples in vitro
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2019
Muhammad Salman, Rizwana Rizwana, Hayat Khan, Iqbal Munir, Muhammad Hamayun, Aquib Iqbal, Abdul Rehman, Khalid Amin, Ghayour Ahmed, Majid Khan, Ajmal Khan, Faiz Ul Amin
A total of 500 bacterial samples from superficial wounds, scrapes, cuts, incisions and burns were collected using sterile cotton swab without contaminating it with skin commensals. Samples were streaked on McConekey agar, nutrient agar, cetrimide agar and blood agar. The bacterial growth was observed on these media for the colony morphology. For further identification and characterization, these isolates were subjected to microscopic examination, Gram staining, biochemical assays and finally confirmed by 16Sribosomal RNA technique.