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High-Performance Liquid Chromatography
Published in Adorjan Aszalos, Modern Analysis of Antibiotics, 2020
Joel J. Kirschbaum, Adorjan Aszalos
Amoxicillin, ampicillin, etacillin, oxacillin, cloxacillin, flucloxacillin, and cicloxacillin eluted in this order, as did cephaloridine, cephacetrile, cephazolin, cephapirin, cephradine, and cephalothin, using an octadecylsilane column and a mobile phase of 0.1 M borate buffer, pH 8. 5-n-propanol, containing 0.6% cetyltrimethylammonium bromide, flowing at 1 ml/min into a detector set to 252 nm [148].
Ecology
Published in Paul Pumpens, Single-Stranded RNA Phages, 2020
Chitosan, a natural nontoxic biodegradable polymer obtained by the deacetylation of chitin from the exoskeleton of crustaceans, decreased pfu of the phage MS2 and feline calicivirus, but not of murine norovirus (Su et al. 2009; Davis et al. 2012). Furthermore, the MS2-inhibiting effect of chitosan was confirmed, but no effect was found with such cationic compounds as cetyltrimethylammonium bromide, nisin, and lysozyme (Ly-Chatain et al. 2013). Davis et al. (2015) demonstrated a full picture of the antiviral chitosan activity, where two chitosans of 53 and 222 kDa were tested and chitosan treatments showed the greatest reduction of the phage MS2, followed by feline calicivirus, the phage φX174, and murine norovirus.
Considering Tin as a Vital Nutrient
Published in Nate F. Cardarelli, Tin as a Vital Nutrient:, 2019
The speciation of unknown organic tin components of human and other mammalian tissue, at concentrations of 0.01 ppm or lower, presents difficulties beyond the present state-of-the-art as regards the above methodologies. The speciation of ultralow tissue tin is necessary for essential cancer prophylaxis work as will be described in later sections of this text. Technology has yet to arrive at that state. At this time we rely on two essential techniques. The phenylfluorone method, as described by Smith,86 was enhanced with cetyltrimethylammonium bromide,103 coupled with extraction techniques and other modifications as described by Sherman et al.104,105 In tracing the movement of tin in the mammalian body and its presence in specific organ extracts, the readily available 113Sn isotope is used, as will be described in a later chapter.
Effect and mechanism of paclitaxel loaded on magnetic Fe3O4@mSiO2-NH2-FA nanocomposites to MCF-7 cells
Published in Drug Delivery, 2023
Yun Ni, Peng Deng, Ruitong Yin, Ziye Zhu, Chen Ling, Mingyi Ma, Jie Wang, Shasha Li, Ruijiang Liu
Cetyltrimethylammonium bromide (CTAB) (1.0 g) was added into 180 mL of deionized water containing 2 mL ammonium hydroxide and dissolved completely using ultrasound. Next, 35 mL diethyl ether and 50 mL ethanol were added into the mixture and stirred continuously at room temperature (25 °C). Magnetic Fe3O4 nanoparticles (0.2 g) were dispersed in anhydrous ethanol (10 mL) by ultrasound. Tetraethyl orthosilicate (TEOS) (2 mL) was added after stirring for 10 min, followed by vigorous stirring at room temperature for 2 h. The obtained products were separated under an external magnetic field, washed with deionized water and ethanol, and vacuum dried at 70 °C for 12 h. The products were then dispersed in 50 mL of acidic ethanol and refluxed for 8 h (Fang et al. 2019). This process was repeated three times. Magnetic Fe3O4@mSiO2 nanocomposites were obtained via centrifugation, washing, and drying. Acidic ethanol contained 1% glacial acetic acid. The magnetic Fe3O4@mSiO2 nanocomposites were characterized using FTIR, dynamic light scattering (DLS), SEM, TEM, BET, and vibrating sample magnetometer (VSM).
Development of local anesthetic drug delivery system by administration of organo-silica nanoformulations under ultrasound stimuli: in vitro and in vivo investigations
Published in Drug Delivery, 2021
Rong-Qin Qi, Wei Liu, Duan-Yu Wang, Fan-Qing Meng, Hong-Ying Wang, Hai-Yan Qi
In a characteristic reaction, 1000 mg of cetyltrimethylammonium bromide was broken down in double distilled water under sonication and the solution temperature was acclimated to 100 °C. At that point, 2 M of NaOH solution was added to the cetyltrimethylammonium bromide arrangement. After consistent mixing, 5 mL of tetraethyl orthosilicate was included drop into the cetyltrimethylammonium bromide arrangement, and the reaction proceeded for another half a day. At that point, the strong rough item was acquired after the reaction blend was kept for in any event one day under calm conditions. This solid unrefined item was along these lines centrifuged, washed with distilled water and alcohol a few times and dried in a vacuum at 100 °C medium-term to get the free MONPs. 0.5 g of MONPs was centrifuged in the wake of refluxing in an alcohol hydrochloric acid arrangement at 50 °C for half a day to evacuate cetyltrimethylammonium bromide and was washed with DD and alcohol a few times and dried in hot air oven 80 °C for 24 h (Giraldo et al., 2007; Ma et al., 2013).
Thermal analysis of laser irradiation-gold nanorod combinations at 808 nm, 940 nm, 975 nm and 1064 nm wavelengths in breast cancer model
Published in International Journal of Hyperthermia, 2021
Leonardo Bianchi, Rachael Mooney, Yvonne R. Cornejo, Emiliano Schena, Jacob M. Berlin, Karen S. Aboody, Paola Saccomandi
GNRs were supplied by Nanopartz™, a division of Concurrent Analytical, Inc. The GNRs surface coating was selected to be 11-mercaptoundecyltrimethylammonnium bromide (MUTAB). MUTAB was preferred over other surface coatings, such as cetyltrimethylammonium bromide (CTAB), to avoid cytotoxicity issues due to the surfactant [20], since CTAB results toxic when dissociated from the GNRs [10,32]. Furthermore, MUTAB exploits peculiar characteristics thanks to the intrinsic capabilities to covalently bind to GNRs and favor the cellular uptake, due to its cationic charge [33], which makes this coating a valid candidate for biocompatible nanostructures. Four different laser wavelengths were used in the present study, i.e., 808 nm, 940 nm, 975 nm, and 1064 nm, therefore a specific GNR type was injected according to each specific wavelength. Thus, the aspect ratio of the nanostructure was selected in line with the wavelength of laser light to exploit the desired surface plasmon resonance (SPR phenomenon) [34]. The characteristics of the GNRs selected for each wavelength of the laser emitters are presented in Table 1. The GNR size, i.e., diameter (D) and length (l), the aspect ratio (AR) and the zeta potential (ζ potential) for GNRs dispersed in an 18 MOhm deionized water are displayed.