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Artemisia annua and Its Bioactive Compounds as Anti-Inflammatory Agents
Published in Tariq Aftab, M. Naeem, M. Masroor, A. Khan, Artemisia annua, 2017
Bianca Ivanescu, Andreia Corciova
To determine casticin and artemetin by GC/MS, Weathers and Towler (2012) used derivatization by adding pyridine, BSTFA-N,O-bis(trimethylsilyl)trifluoroacetamide, and TMCS-trimethylsilyl chloride, heating at 70° C for 4 hours. Chromatographic conditions included: Helium (He) as carrier gas at 1 mL/min, injection volumes in splitless mode, ion source temperature at 275° C, and oven temperature increased from 110° C at 280° C. In case of artemisinin, derivatization was not necessary.
Metabolomics reveals the depletion of intracellular metabolites in HepG2 cells after treatment with gold nanoparticles
Published in Nanotoxicology, 2018
Jeremie Zander Lindeque, Alnari Matthyser, Shayne Mason, Roan Louw, Cornelius Johannes Francois Taute
For untargeted GC-MS analysis, the dried cell extracts were oximated with the addition of 50 µl methoxyamine hydrochloride (2 mg/ml pyridine). After 1 h incubation at 50 °C, the samples were allowed to cool down for 5 min before adding a silylation agent. One hundred microliters of N,O-Bistrifluoroacetamide (BSTFA) with 1% trimethylsilyl chloride (TMCS) were added to each sample and incubated again at 50 °C for 1 h. GC-MS analyses were performed on a GC-TOF-MS system which consisted of an Agilent 7890 A GC front-end system and a Leco Pegasus HT TOFMS with an Agilent 7693 autosampler. The samples were separated with an RTX-1 MS column (30 m × 250 µm × 0.25 µm) from Restek. Two microliters of the sample was injected in splitless mode. Helium was used as a carrier gas with a constant flow rate of 1.5 ml/minute. The front inlet temperature remained 250 °C during injection. The GC oven initiated at a temperature of 70 °C for 1 min, after which the temperature was increased stepwise to 120 °C at 7 °C/min, then again increased to 230 °C at 10 °C/min, and finally increased to 300 °C at 13 °C/min. The GC oven remained 300 °C for 1 min before cooling down and returning to its initial temperature. The transfer line remained at a constant temperature of 225 °C and the source remained 200 °C. Electron impact ionization was performed at −70 V to fragment the compounds that eluted. An acquisition delay of 450 s was allowed before the data was acquired at 20 spectra/s (50–950 m/z).
Cytotoxic furanosesquiterpenoids and steroids from Ircinia mutans sponges
Published in Pharmaceutical Biology, 2021
Fatemeh Heidary Jamebozorgi, Morteza Yousefzadi, Omidreza Firuzi, Melika Nazemi, Somayeh Zare, Jima N. Chandran, Bernd Schneider, Ian T. Baldwin, Amir Reza Jassbi
In order to quantify the steroid contents in the sub-fractions FW9-10- F51-80 of the winter sample by GC-MS, fraction constituents were transformed to trimethylsilyl derivatives. Briefly, 200 μL of the reagent, N-O-bis-(trimethylsilyl)-trifluoroacetamide (BSTFA) containing 1% trimethylsilyl chloride (TMCS) was added to 2 mg of F9,10 -F51-80 and vortexed well. Afterwards, the mixture was heated in a hot water bath (60 °C) for 1 h. Samples were evaporated under a stream of nitrogen gas, redissolved in 200 μL DCM and the resulting solution was subjected to GC-MS as previously described (Jassbi et al. 2013).