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Value-Added Products and Bioactive Compounds from Fruit Wastes
Published in Megh R. Goyal, Arijit Nath, Rasul Hafiz Ansar Suleria, Plant-Based Functional Foods and Phytochemicals, 2021
Ranjay Kumar Thakur, Rahel Suchintita Das, Prashant K. Biswas, Mukesh Singh
The pineapple by-products also contain a significant amount of dietary fiber, mainly insoluble dietary fiber, and can be used for the development of food-grade low-calorie fiber-enriched food products [68]. Crown of pineapple was used as the carbon source for the media for the growth of three different strains, namely, Rhodopseudomonas Saccharomyces cerevisiae (baker’s yeast), and Trichoderma harzianum. These strains were used for microbial single-cell protein production [118].
Protein and amino acids
Published in Geoffrey P. Webb, Nutrition, 2019
In the 1950s, 1960s and the 1970s, there were many very expensive projects aimed at increasing protein supplies from alternative sources to try and bridge the growing gap between the human requirement for protein and the worldwide supply. Billions of pounds at today’s prices were invested in these projects and as they were aimed primarily at solving an illusory problem (i.e. closing the protein gap) so most of this money and effort was wasted. These measures and their cost were discussed in a book by Kenneth Carpenter (1994) entitled Protein and Energy and some of these projects are listed as follows. Projects to develop cheap and palatable high-protein foods that could be produced from locally available products like fishmeal, soybeans, peanuts, coconut, sesame and cotton seeds. The development of single cell protein (SCP) from yeasts, algae and bacteria. Most of these projects never came to fruition, but Quorn is one successful result of such programmes; it is, however, now marketed as an alternative to meat for affluent vegetarians rather than a cheap protein supply for Third-World protein-deficient children. Companies like BP initiated major research and development programmes to produce SCP from by-products of the oil refining process. The breeding of genetically enhanced plants with higher and better quality protein content; these generally gave lower yields and required more fertiliser inputs and so none of those developed were ever widely used. Addition of synthetic amino acids to plant proteins like wheat to increase their quality.
Multiplex protein analysis for the study of glaucoma
Published in Expert Review of Proteomics, 2021
A related field is evolving for multiplex and high-throughput analysis of proteins in single-cells [40–42]. Despite increasingly available technologies for gene expression profiling by single-cell or nucleus sequencing, single-cell applications for protein analysis are still premature. Development of single-cell proteomics may take a while; however, its realization is not a distant dream, as many laboratories are already accomplishing to decrease the sample volume requirements for a suitable resolution. Among continuously emerging strategies, mass cytometry by time-of-flight presents an analysis technique combining flow cytometry and mass spectrometry. Using a pool of isotope-conjugated antibodies, this technique permits to distinguish different subpopulations based on co-expression of various proteins in a specific cell type. While mass cytometry decodes cellular heterogeneity for detection of proteins, their expression levels, and post-translational modifications in single-cell suspensions, imaging mass cytometry combines the mass cytometry with immunohistochemistry techniques to investigate samples in the solid state and to acquire spatial data on tissue sections [43,44]. Applications of these single-cell techniques are increasing to improve the understanding of different brain pathologies [45,46]. The vast progress in single-cell protein research is similarly promising for exclusive opportunities in future studies of glaucoma research.
Chemical Utilization of Albizia lebbeck Leaves for Developing Protein Concentrates as a Dietary Supplement
Published in Journal of Dietary Supplements, 2018
Lutful Haque Khan, V. K. Varshney
Proteins are most important for the development of the human body and maintenance of good health. Adequate amounts of proteins are essential for normal maintenance of body functions and for growth, maturation, pregnancy, lactation, and recovery from injuries and diseases. Protein deficiency and malnutrition are being experienced by a large population of the developing world. With rapid population growth coupled with limited cultivable land, shortage of protein has become a global issue. The world has recognized the grim truth that ultimately the population growth will outstrip food suppliers. Approximately 60% of the 10.9 million deaths each year among children under the age of five in the developing world are attributed to protein malnutrition (WHO, 2002). Further, shortage of good quality protein is a serious concern of feed producers. These considerations have necessitated the search for additional sources of protein. Several novel sources of protein such as fish protein concentrate (FPC; Bárzana & Garía-Garibay, 1994), single-cell protein (SCP; Nasseri et al., 2011), soybean protein (SBP; Bhatia & Greer, 2008), and insect protein (Ghaly & Alkoaik, 2010) have been suggested to meet the increasing demand of human dietary and animal protein.
Understanding Leishmania parasites through proteomics and implications for the clinic
Published in Expert Review of Proteomics, 2018
Single-cell protein analysis with advanced microfluid platforms represents another avenue for understanding disease immunobiology at the single-cell level. However, the widespread use of single-cell analysis requires the support of modelling and informatics tools that can integrate protein datasets with other omics tools. Thus, proteomics remains important to depict parasite biology, disease pathogenesis and immune responses. Analyses of subproteomes, exoproteomes and posttranslational modifications have unveiled several structural as well as functional aspects of infection. Chip/microarray-based proteomic platforms have potentially raised concerns for identifying novel protein targets for therapeutic intervention, while proteome mapping and/or their combination with other post-genomics have contributed to our understanding of several untouched aspects of host-parasite interactions, disease pathogenesis and drug resistance mechanisms. The advent of newer proteomic tools will serve to establish the framework for disease diagnostics, treatment and vaccine development.