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Restorative Proctocolectomy in Colitis
Published in Peter Sagar, Andrew G. Hill, Charles H. Knowles, Stefan Post, Willem A. Bemelman, Patricia L. Roberts, Susan Galandiuk, John R.T. Monson, Michael R.B. Keighley, Norman S. Williams, Keighley & Williams’ Surgery of the Anus, Rectum and Colon, 2019
Colin Peirce, Feza Remzi, Michael R.B. Keighley
Management of PVF is best approached by first addressing any sepsis present in the pouch, vagina or perineal body using gentle debridement. Pouch-anal sepsis can initially be controlled with seton insertion. Once sepsis and inflammation are cleared, PVF may be managed surgically. A low, short PVF associated with healthy tissue may be addressed by transanal or transvaginal advancement flap closure.98 If CD is suspected, one may initiate biologic therapy and reassess the tissue quality at a later date with the possible option of surgical closure at that time. Use of covering ileostomy in these cases is greatly debated; however, this would be used routinely. Transabdominal IPAA revision is a good option in selected cases with good results and may be offered after a long and honest discussion about the morbidity of surgery, the possibility of pouch loss and possibility of a recurrent PVF.99
Noninvasive detection of COPD and Lung Cancer through breath analysis using MOS Sensor array based e-nose
Published in Expert Review of Molecular Diagnostics, 2021
Binson V A, M. Subramoniam, Luke Mathew
The participants selected for the study were advised to fast for a period of two hours prior to breath sampling. Tobacco smoking, mouthwashes, toothpastes, and medications were not allowed. They were asked to wait for 2 hours in a closed room to ensure that there is no interaction with exogenous VOCs and all the exhaled breath VOCs are a result of endogenous processes. The temperature inside the room is regulated at 23°C and relative humidity is also maintained between 30% and 50%. A constant heater voltage of 5 V was used to preheat MOS sensors and the repetition of this procedure was performed before each sample detection. Breath samples were collected in a 1 L Tedlar bag made of Polyvinyl fluoride (PVF). To eliminate the dead space air and collect the alveolar breath, the first collected breath was discarded [41,42]. The expelled breath samples of volunteers under study were collected in the Tedlar bag through a tube attached to it. The samples were collected from the subjects for few seconds in such a way the entire disease biomarker VOCs was collected in the sample holder. After each measurement, the sensor chamber is cleaned by applying pure nitrogen to it. The sampling circumstances were uncontrolled, ie., the impact of breath-hold, expiratory flow rate, and dead space inclusion were not considered [43].
Efficacy of different straw phonation doses for vocal fatigue prevention
Published in International Journal of Speech-Language Pathology, 2021
Elaine Kwong, Suen Yue Sarah Poon, Cheuk Yiu Tse
The self-reported PVF score is reflective of the severity of VF, which is believed to be identified by a set of relevant symptoms (Nanjundeswaran et al., 2015), from the participants' perspective. Among the seven treatment conditions, the only significant Pre-treatment–Post-treatment improvement was observed in the Voice rest condition. Voice rest for 15 minutes with hydration was able to alleviate VF symptoms originated from daily vocal demand that had occurred before their visit, although the visits were not scheduled at about the same time of the day. Furthermore, as the participants were required to rate the self-perceived severity on a list of VF symptoms and those symptoms would typically improve with rest (Kitch & Oates, 1994; Nanjundeswaran et al., 2015; Solomon, 2008), it was not surprising that the PVF measure was sensitive to the changes occurred in the Voice rest condition. The significant Pre-treatment–Post-treatment deterioration in PVF in the Air-3 and Water-5 conditions, on the other hand, suggested that these two doses of straw phonation may be VF-inducing, even from the participants' perspective.
SP1-induced up-regulation of lncRNA LUCAT1 promotes proliferation, migration and invasion of cervical cancer by sponging miR-181a
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2019
Liang Zhang, Shi-Kai Liu, Lili Song, Hai-Rong Yao
A total protein extraction kit (GeneLife, Mentougou, Beijing, China) was applied to lyse the HeLa and SiHa cells. Then, 20 μg of protein was loaded onto the SDS-PAGE and sequentially transferred to a polyvinyl fluoride membrane. After being blocked with 5% non-fat milk, the membranes were probed with primary and secondary antibodies. Finally, an ECL Western Blotting Substrate kit (ZeYe Biotech, Jinshan, Shanghai, China) and a ChemiScope 6000 system (CLiNX, Baoshan, Shanghai, China) were utilized to detect the protein bands. The primary antibodies against caspase-3, vimentin and caspase-9 were purchased from Sino Biological Inc. (Yizhuang, Beijing, China). The primary antibodies targeting N-Cadherin and GAPDH were obtained from Boster Biotechnology Co., Ltd. (Wuhan, Hubei, China).