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Caesarean Section
Published in Gowri Dorairajan, Management of Normal and High Risk Labour During Childbirth, 2022
The second approach is to ligate the anterior division of the hypogastric artery of the same side first and then empty the hematoma, especially when the hematoma is large. Sometimes it may be necessary to ligate the uterine artery at its origin from the internal iliac artery. Uterine vein injury has to be dealt with similarly by identifying the venous plexus and ligating them carefully proximally. Tamponade with surgical mops left for 24–48 hours is the last resort. It is not followed anymore as this procedure needs a relaparotomy to remove the mops. Also, as the bleeding is traumatic, it is amenable to primary haemostatic suturing. The operating surgeon must be well versed with uterine artery and internal iliac artery ligation or should take prompt help from those with expertise when needed. It is a good dictum to delineate the course of the ureter once the bleeding has been controlled to rule out any ureteric injury/ligation. A cystoscopy can also be done to look at the free intravesical spurting of urine from both ureters.
Isolated, Perfused Microvessels
Published in John H. Barker, Gary L. Anderson, Michael D. Menger, Clinically Applied Microcirculation Research, 2019
Michael J. Davis, Lih Kuo, William M. Chilian, Judy M. Muller
We typically study isolated microvessels in a physiological salt solution buffered with MOPS (morpholinopropanesulfonic acid), although other buffer systems have been and could be used.10,36 A detailed description of the MOPS solution is given by Duling et al.16 We always use highly purified or double-distilled water to prepare all solutions. Small amounts of EGTA are important, probably to chelate residual heavy metal ions that may be present (E. VanBavel, personal communication).
Preparation and evaluation of oocytes for intracytoplasmic sperm injection
Published in David K. Gardner, Ariel Weissman, Colin M. Howles, Zeev Shoham, Textbook of Assisted Reproductive Techniques, 2017
A special shallow Falcon dish (type 1006) is used for placing the denuded eggs. Nine small droplets of MOPS (3-(N-morpholino)propanesulfonic acid)-HEPES-buffered human tubal fluid culture media (MHM, Irvine Scientific, CA, USA), containing 10% synthetic serum, 5 μL each, are arranged in a square of 3 x 3 within this dish. An additional 10th droplet serves for orientation. The middle droplet, in which the sperm will be placed, contains 7%-10% polyvinylpyrrolidone (PVP). The droplets are then covered with either paraffin or mineral oil, and the dish is placed on the heated area in the hood to warm up before removal of the cumulus cells.
Untargeted lipidomic differences between clinical strains of methicillin-sensitive and methicillin-resistant Staphylococcus aureus
Published in Infectious Diseases, 2022
Philip Nikolic, Poonam Mudgil, David G. Harman, John Whitehall
Whole cell surface charge was determined using a cytochrome c biding assay based on Matsuo et al. [24] and Meehl et al. [25]. In brief, cells were incubated overnight in MHB on a shaking incubator (100 rpm, 35 °C) to a final concentration of ≈109 CFU/mL. The cells were pelleted and washed twice in 20 mM morpholinepropanesulfonic acid (MOPS), pH 7, resuspended in 0.25 mg/mL cytochrome c, in MOPS and incubated in the dark at room temperature for 10 min. The cells were centrifuged and the amount of cytochrome c in the supernatant determined at OD530 using a standard curve. Three independent experiments were run for all ten strains. The percent bound cytochrome c was compared between the MSSA group and MRSA group using a Welch Two Sample t-test conducted in R at a significance of p ≤ .05 and between each strain using a one-way ANOVA at a significance of p ≤ .05.
Effects of a new magnesium-rich artificial cerebrospinal fluid on contractile 5-hydroxytryptamine and endothelin receptors in rat cerebral arteries
Published in Neurological Research, 2019
Ya-Wen Cheng, Yi-Chen Guo, Guo-Liang Li, Yong-Ning Deng, Wen-Juan Li, Gao-Feng Xu, Zhong Deng, Yong-Xiao Cao, Guo-Gang Luo
Rats were anaesthetized with CO2 and decapitated. The brains were immediately removed and put in ice-cold 3-(N-morpholino)propanesulfonic acid (MOPS) fluid. The constituents of the MOPS fluid variants used in the study are shown in Table 1. The basilar arteries were isolated under a dissection microscope, cut into ring segments of 1 mm in length and placed in a culture plate containing 1 ml medium (clear CSF, haemorrhagic CSF or MACSF) with two kinds of antibiotics (1 × 105 u/L penicillin and 100 mg/L streptomycin). The arterial segments were incubated at 37°C in humidified 5% CO2 and 95% air and assessed at 6, 12 and 24 hours. Then, the rat basilar arteries were used for contractility analyses via myograph or frozen in liquid nitrogen for messenger ribonucleic acid (mRNA) and protein analyses.
Differential transformation and antibacterial effects of silver nanoparticles in aerobic and anaerobic environment
Published in Nanotoxicology, 2019
A Pseudomonas aeruginosa PAO1 strain from our laboratory was used. LB or MOPS media (LaBauve & Wargo 2012) was used to grow P. aeruginosa cells. To reduce the changes of AgNPs in the media, MOPS medium was modified to minimize the reaction of Ag with media components. The MOPS salts solution includes (pH, 7.2): 40 mM MOPS, 50 mM NaHCO3, 9.5 mM NH4HCO3, 1.9 mM K2HPO4, 0.5 mM MgSO4, 0.5 µM CaCl2, and trace elements (Widdel, Kohring, & Mayer 1983). Yeast extract (0.001%, w/w) and KNO3 (100 mM) was additionally supplied to support bacterial growth in anaerobic condition (Hunt and Phibbs 1983; Williams et al. 1978). Glucose (50 mM) was used as carbon source for the culturing in aerobic and anaerobic condition.