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Autoradiography
Published in Howard J. Glenn, Lelio G. Colombetti, Biologic Applications of Radiotracers, 2019
Sven Ullberg, Bengt Larsson, Hans Tjälve
The centrifugation technique involves that sections are placed on a flat surface close to the bottom of a centrifuge tube56 which is then in the darkroom filled with a melted and strongly diluted emulsion. The silver bromide grains are then centrifuged down over the sections. This method gives a very even layer of tightly packed grains and a small quantity of gelation. It is however so laborious that it has not been frequently used.
Technologies for Separation and Drying of Algal Biomass for Varied Applications
Published in Gokare A. Ravishankar, Ranga Rao Ambati, Handbook of Algal Technologies and Phytochemicals, 2019
Julio Cesar de Carvalho, Eduardo Bittencourt Sydney, Paulo Cesar de Souza Kirnev, Adriane Bianchi Pedroni Medeiros, Carlos Ricardo Soccol
Centrifugation is the use of the centrifugal force to promote insoluble particles settling in a solid–liquid mixture. It is a popular operation for biomass recovery, especially when the aggregated value justifies the operation – as is the case for the production of astaxanthin from Haematococcus pluvialis. In pilot/industrial scales of microalgae production centrifuges of many types are used:
Granulocyte and Monocyte Antigens and Antibodies
Published in Soldano Ferrone, B. G. Solheim, HLA Typing: Methodology and Clinical Aspects, 2019
J. S. Thompson, C. D. Serverson, N. E. Goeken, J. Rhoades
Collect the leukocyte-rich supernatant, add PBS to ¾ full volume, mix and under-layer with 3.0 mℓ of cold Hypaque-Ficoll density gradient (sp gr = 1.077 at room temperature). Centrifuge at 700 × g for 20 min in the clinical centrifuge.
Triptorelin nanoparticle-loaded microneedles for use in assisted reproductive technology
Published in Drug Delivery, 2023
Xiaoyan Lu, Yiying Sun, Meishan Han, Daoyuan Chen, Xiaoyan He, Siqi Wang, Kaoxiang Sun
The NPs-MNs were manufactured using a twin-casting method, as described previously (Lu et al., 2022). NPs were added to a polydimethylsiloxane (PDMS) mold (10 × 10 array) and centrifuged into the tip groove. After drying and formation, PAA solution was added and centrifugation was undertaken again. Both centrifugations were carried out at 2800 rpm for 30 min at 10 °C using a centrifuge (5810R; Eppendorf, Hamburg, Germany). Then, the NPs-MNs were dried for 72 h at 25 °C, then demolded and stored in a dehumidifier. Stereographic microscopy using a SZN71 system (Sunny Optical Technology, Beijing, China) was undertaken to observe the morphology of NPs-MNs. Scanning electron microscopy (SEM) using an XL G2 setup (Phenom Scientific, Amsterdam, The Netherlands) was employed to observe the fine structure of individual MNs.
Impact of cerebral microbleeds on cognitive functions and its risk factors in acute cerebral infarction patients
Published in Neurological Research, 2023
Linyun Chen, Feng Liu, Xuan Tian, Tian Zhang, Jian Zhang, Fang Ran
Five milliliters of fasting cubital venous blood was collected from patients on the day of admission (the control group was on the day of physical examination) and added to an anticoagulation tube containing disodium ethylene diamine tetra-acetic acid. Serum samples were obtained by centrifugation with a Centrifuge 5418 R desktop multi-function centrifuge from Eppendorf AG, Germany. The double antibody sandwich method was used to detect the serum D-dimer level, and the chemiluminescence method was used to detect the serum S100β and neuron-specific enolase (NSE) levels. The NSE detector was Zhengzhou Antu automatic Chemiluminescence instrument A2000PULS, S100β detector was Shandong Kanghua automatic chemiluminescence instrument AXCEED400T. High-sensitive C-reactive protein (hs-CRP) was detected by rate nephelometry using Beckman L×20 automatic microprotein analyzer.
ADME properties of CHF6366, a novel bi-functional M3 muscarinic receptor antagonist and ß2 adrenoceptor agonist (MABA) radiolabelled at both functional moieties
Published in Xenobiotica, 2023
Alberto Ghiglieri, Monica Messina, Valentina Cenacchi, Claudia Piutti, Flavio Cinato, Giandomenico Brogin, Paola Puccini
Single incubation of [14C]-CHF6366 (2.15 GBq for each labelling position) was performed at the concentration of 10 µM with mouse, rat, rabbit, dog, monkey and human liver microsomes (0.8 mg/mL protein content for each species) in Dulbecco’s buffer, pH 7.4, at 37 °C in the presence of NADPH (1 mM). At t = 0 and after 20 and 60 min incubation, aliquots of the incubates were taken and the metabolism was stopped adding chilled acetonitrile. The stability of [14C]-CHF6366 was checked in parallel by incubating the radiolabelled compound for 60 min with thermally inactivated liver microsomes. The vitality of cryopreserved hepatocytes of the above-mentioned species, after thawing, was determined by the Trypan Blue exclusion method. [14C]-CHF6366 was incubated, for each species in single, at the concentration of 10 µM with these hepatocytes in Leibovitz L-15 medium at 37 °C. At t = 0, and after 30 and 90 min incubation, aliquots were taken, and the metabolism was stopped adding chilled acetonitrile. The resulting mixtures were centrifuged. Incubation supernatants were evaporated to dryness and reconstituted with an appropriate mixture of solvent A and solvent B (8:2, v:v), according to the HPLC conditions optimised for the elution of each labelled position and submitted to radio-HPLC analysis.