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Protoplasts as Tools in the Study of Moss Development
Published in R. N. Chopra, Satish C. Bhatla, Bryophyte Development: Physiology and Biochemistry, 2019
Neil W. Ashton, Philip J. Boyd, David J. Cove, Celia D. Knight
This technique has further potential in the mutagenesis of P. patens, either by the process of “transposon tagging”37,38 or by simple insertional inactivation by the transforming plasmid DNA. The advantage of this form of mutagenesis is that the inactivated gene could be recovered for sequence analysis.
John Sulston (1942–2018): a personal perspective
Published in Journal of Neurogenetics, 2020
Robert H. Waterston, Donald G. Moerman
That sense of community and collaboration pervaded the worm mapping effort. John saw the physical map as a collaboration between his and Alan’s work to establish clone overlaps and the entire C. elegans community as they used mutants to discover genes of consequence. When members of the community would locate their genes on the genetic map, John and Alan would ship the group clones that spanned the interval, all prepublication. Once the gene was located in that interval it became another anchor between the genetic and clone maps, creating a true genome map. Or if someone had cloned a gene by homology or transposon tagging, they would send their cloned DNA to John and Alan, who would find its location on the clone map and by inference the genetic map, creating still more landmarks (Figure 3). The tradition in the worm community, fostered by the Worm Breeder’s Gazette, was one of willing sharing of data and ideas before publication, with the understanding that individuals would not unfairly exploit that prepublication access. They exploited the emerging ability to communicate across computers by posting the map on host computers in Cambridge, Boston, St. Louis and Los Angeles and allowing people to access the data via dialup connections. By the time the nearly completed map was presented at the biennial worm meeting at CSH in 1989, they could paste almost all the pieces in order along each of the six chromosomes.