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Replicase
Published in Paul Pumpens, Single-Stranded RNA Phages, 2020
Meanwhile, the Hfq protein was found to bind both supercoiled and linear plasmid DNA, but the binding seemed to be sequence-nonspecific (Takada et al. 1997). Among other E. coli DNA-binding proteins, the Hfq appeared as a curved DNA-binding protein with the highest preference for curved DNA (Ali Azam and Ishihama 1999; Ali Azam et al. 1999). It led to an attractive hypothesis that the Hfq with binding activities to both DNA and RNA could play a role in the functional coordination between DNA and RNA such as transcription-translation coupling (Ali Azam and Ishihama 1999). Moreover, in tight connection with the RNA phage activity, the Hfq was qualified as a regulator of the F-plasmid TraJ and TraM synthesis in E. coli (Will and Frost 2006), encoded by the genes that were described generally in the Genetics of F pili section in Chapter 3.
Riboswitches as therapeutic targets: promise of a new era of antibiotics
Published in Expert Opinion on Therapeutic Targets, 2023
Emily Ellinger, Adrien Chauvier, Rosa A. Romero, Yichen Liu, Sujay Ray, Nils G. Walter
In recent work, a preQ1-sensing translational riboswitch from B. anthracis was found to modulate transcription-translation coupling upon ligand binding by altering the likelihood of ribosome recruitment [107]. By surveying riboswitch transcription in real-time, the Walter group found that the ribosome positively promotes transcription elongation in the absence of ligand. In contrast, the presence of cognate ligand led to a similar RNAP transcription rate with and without ribosome, suggesting that translation initiation in the wake of RNAP is controlled by ligand binding to the riboswitch. These findings open the door to the design of new antibiotics targeting the ribosome-RNAP, riboswitch-ribosome, riboswitch-RNAP or riboswitch-RNAP-ribosome interfaces.