China
Africa
Explore chapters and articles related to this topic
The Role of Nutrition and Nutritional Supplements in the Treatment of Dyslipidemia
Published in Stephen T. Sinatra, Mark C. Houston, Nutritional and Integrative Strategies in Cardiovascular Medicine, 2015
Expanded lipid profiles that measure lipids, lipid subfractions, particle size and number, and APO-B and A are preferred to standard obsolete lipid profiles that measure only the TC, LDL, TG, or HDL. These expanded lipid profiles such as the LPP (lipoprotein particles; Spectracell labs), NMR (nuclear magnetic resonance) (LipoScience, Raleigh, North Carolina), Berkley Heart Labs and VAP (vertical auto profile) (Atherotec, Birmingham, Alabama) improve serum lipid analysis and CHD risk profiling.23,24
Lipoprotein Metabolism and Implications for Atherosclerosis Risk Determination and Treatment Decisions
Published in P. K. Shah, Risk Factors in Coronary Artery Disease, 2006
H. Robert Superko, Szilard Voros, Spencer King III
The gold-standard laboratory method of determining lipoprotein subclass distribution is based on density as determined in the ANUC (4). This method employs a highly accurate and reproducible ultracentrifugation method that characterizes lipoprotein subclasses by floatation intervals. It is time consuming, expensive, and available only in a limited number of research laboratories. Nondenaturing GGE was developed as a less expensive method of determining lipoprotein subclass distribution (19). A rapid ultracentrifugation method, termed vertical auto profile (VAP), has been used to determine relative floatation index as a determination of change in LDL buoyancy (140). This method determines the cholesterol concentration of multiple lipoprotein fractions based on density. During profile decomposition, peak heights for predefined sub-curves for all classes are simultaneously varied until the sum of the squared deviations between the sum of the sub-curves and the parent profile is minimized using linear regression. A relatively new method used to estimate lipoprotein subclass distribution is nuclear magnetic resonance (NMR) (141). Signals are derived from methyl groups on phospholipids, cholesterol, cholesterol ester, and triglycerides. NMR assumes a constancy of lipid mass contained within a particle of given diameter and phospholipid composition and thus methyl lipid NMR signal. This system uses a library of reference spectra of lipoprotein subclasses incorporated into a linear least-square fitting computer program which works backward from the shape of the composite plasma methyl signal to compute the subclass signal intensities.
Potential role of anti-inflammatory HDL subclasses in metabolic unhealth/obesity
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2021
Hongjuan Tang, Zhicong Xiang, Longyu Li, Xiaofei Shao, Qin Zhou, Xu You, Chongxiang Xiong, Jing Ning, Tong Chen, David Deng, Hequn Zou
Several methods have been developed to partition HDL into various subclasses: analytical ultracentrifugation, isopycnic equilibrium, vertical auto profile, two-dimensional gel electrophoresis, nuclear magnetic resonance, and ion mobility [9,29]. However, these methods are labour-intensive, expensive, time-consuming, and restricted to research laboratories for measurements of HDL subfractions [29,30]. We utilize microfluidic chip electrophoresis (MCE), the effectiveness has been proven [31], that is rapid, robust, and easy-to-use for HDL subtyping in people suffering from metabolic unhealth or obesity.
Relationship between TSH Levels and the Advanced Lipoprotein Profile in the Brazilian Longitudinal Study of Adult Health (ELSA–Brasil)
Published in Endocrine Research, 2020
Érique José F.Peixoto de Miranda, Alessandra C. Goulart, Márcio Sommer Bittencourt, Raul D. Santos, Michael J. Blaha, Steven Jones, Peter P. Toth, Krishnaji Kulkarni, Itamar S. Santos, Paulo A. Lotufo, Isabela M. Bensenor
Despite previous knowledge about lipids and thyroid disease, little is known about the relationship between TSH levels and lipoprotein subfractions measured by newer separation methods. The vertical auto profile (VAP) method11 measures cholesterol in a broad range of atherogenic lipoprotein particles such as triglyceride-rich lipoproteins (TRL-C), and LDL and HDL subfractions. Alterations in the circulating levels of these lipoprotein subfractions may impact the probability of coronary heart disease development.12