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Quality Control and Quality Assurance in Hematology
Published in Harold R. Schumacher, William A. Rock, Sanford A. Stass, Handbook of Hematologic Pathology, 2019
Quality control techniques that are visual and not mathematical: Preparation of a thin blood film and its evaluation.Staining of a blood film and its evaluation.Overall microscopic evaluation of a blood film for diagnostic purposes.
The spleen and lymph nodes
Published in Michael Gaunt, Tjun Tang, Stewart Walsh, General Surgery Outpatient Decisions, 2018
Full blood count and blood film provide information on the number of blood cells circulating and the presence of abnormal cell types (as described above). Hypersplenism results in anaemia, leucopenia and/or thrombocytopenia.Hyposplenism results in abnormal red blood cells (Burr cells, target cells, pitted cells); red cell inclusions (Howell-Jolly bodies, siderotic granules); abnormal platelet morphology; thrombocytosis; and leucocytosis (neutrophilia, lymphocytosis, monocytosis).
Microbiological Diagnosis of Parasitic Diseases
Published in Nancy Khardori, Bench to Bedside, 2018
Besides intestinal infections, infections due to haemo-parasites, is the other common and important group of parasitic infections. A number of parasites have at least some of the stages in their life-cycle passing through blood. These stages may be demonstrated by examination of blood specimens, either using whole blood or after concentration procedures. Some of the important blood parasites include Plasmodium spp., Trypanosoma spp., Babesia spp., Leishmania donovanii, and microfilariae of some filarial nematodes. For most of these parasites, examination of stained thin and thick peripheral blood films is considered to be the most useful and recommended diagnostic technique. The blood films may be prepared directly from the specimens, specimens with anti-coagulant, or from concentrated blood specimens. The most preferred stains used are Romanowsky’s stains and include Giemsa, Wright’s, Field’s, and Leishman’s stain.
Detection of abnormal lymphocytes in the peripheral blood of COVID-19 cancer patients: diagnostic and prognostic possibility
Published in Hematology, 2022
Lobna Refaat, Mona S. Abdellateif, Ahmed Bayoumi, Medhat Khafagy, Eman Z. Kandeel, Hend A. Nooh
Peripheral blood (PB) samples were obtained from all participating subjects at admission during the routine workup of the patients. The blood samples were collected in Ethylenediaminetetraacetic acid (EDTA) vacutainers and prepared for undergoing complete blood count (CBC) analysis. Also, differential total leukocyte count (TLC) was done using SYSMEX XN1000 and SYSMEX XT 1800 analyzers, which included an absolute count of lymphocytes, monocytes, and neutrophils, eosinophils, basophils, and immature granulocytes. The immature granulocytes represented an automated count of promyelocytes, myelocytes, and metamyelocytes in the peripheral blood. Peripheral blood (PB) smears were done by spreading one drop of the blood on a slide and stained with Leishman stain. The WBC morphology was analyzed as changes from normal expected/baseline morphology. The blood films were examined by two experienced hematopathologists using Lecia light microscope with 100× oil-immersed magnifications.
Secondary erythrocytosis due to hemoglobin San Diego
Published in Baylor University Medical Center Proceedings, 2021
In December 2014, a 37-year-old Hispanic man with known mild asthma presented to a local physician with a hemoglobin of 19.4 g/dL, platelet count of 178 × 109/L, and white blood cell count of 6.5 × 109/L. He had fatigue and intermittent headaches. Physical examination was unremarkable. Blood film revealed normal cellular morphology. Serum chemistry, erythropoietin, and testosterone were normal. Janus Kinase-2 mutations (including exon 12 and 13) were negative (Table 1). A sleep study ruled out sleep apnea. A bone marrow biopsy revealed normocellular marrow with trilineage hematopoiesis only. A bone marrow cytogenetic study revealed 46, XY in all 20 metaphases. Abdominal ultrasound ruled out masses, cysts, or hepatosplenomegaly. The asthma appeared to be too mild to be the culprit for SE. He was diagnosed with SE of unknown etiology and underwent intermittent therapeutic phlebotomy until mid-2019.
Anemia and transfusion requirements among Ugandan children with severe malaria treated with intravenous artesunate
Published in Pediatric Hematology and Oncology, 2020
Michael T. Hawkes, Robert O. Opoka, Andrea L. Conroy, Robyn E. Elphinstone, Heather A. Hume, Sophie Namasopo, Kevin C. Kain
Hematologic indices (Hb, hematocrit, RBC distribution width, mean corpuscular volume, mean corpuscular Hb, and mean corpuscular Hb concentration) were assessed using automated Beckman Coulter AcT 5 Diff hematology analyser (Beckman Coulter, Inc., Fullerton, CA) at a College of American Pathologists-certified research laboratory (the Makerere University–John’s Hopkins University Core Laboratory in Kampala, Uganda). LDH activity was quantified using a commercial assay according to manufacturer’s instructions (BioVision, Milpitas, CA, USA). Haptoglobin was measured by ELISA according to manufacturer’s instructions (GenWay Biotech, San Diego, CA, USA). Stained peripheral blood films were inspected by an experienced hematology technician and scored using a standardized case record form, blinded to other clinical and hematologic parameters. Grading of polychromasia (none, 1+, 2+, or 3+) was a semi-quantitative scale, but not a standardized index. Testing for ABO/RhD blood grouping and a room temperature cross-match was performed prior to all RBC transfusions. Pre-transfusion screening for irregular antibodies and 37 °C cross-match were not performed. Due to lack of availability at our resource-constrained hospital, additional hematology testing (e.g., absolute reticulocyte count, direct antiglobulin test, bilirubin, and glucose-6-phosphate dehydrogenase activity) were not performed. Furthermore, urine was not tested for hemoglobinuria.