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Principles of thoracic surgery
Published in Mark Davenport, James D. Geiger, Nigel J. Hall, Steven S. Rothenberg, Operative Pediatric Surgery, 2020
Shaun M. Kunisaki, James D. Geiger
A cotton-tip applicator is used to further develop the extrapleural space posteriorly and superiorly in order to further strip the pleura from the chest wall (Figure 8.7). The rib retractor is re-adjusted, and the extrapleural dissection to the posterior mediastinum is completed under direct vision. A moistened gauze sponge can also be used to facilitate this dissection.
Surgical Facilities, Peri-Operative Care, Anesthesia, and Surgical Techniques
Published in Yuehuei H. An, Richard J. Friedman, Animal Models in Orthopaedic Research, 2020
Alison C. Smith, M. Michael Swindle
Following transport to the operating room or table, a “sterile” scrub is performed prior to draping. Three successive scrubs with a surgical disinfectant are performed using sterile gauze sponges, sponge forceps and surgical gloves using the same outward pattern. An application of 70% alcohol follows a suitable contact time for the disinfectant used, then the entire area is dried. It is then preferable to apply a plastic, incisable drape over the entire area just prepared. This type of drape is a better bacterial barrier than cloth drapes because it is impermeable to fluids and prevents wicking of moisture from the periphery into the incision.
Medium-Depth and Deep Peeling
Published in Antonella Tosti, Maria Pia De Padova, Gabriella Fabbrocini, Kenneth R. Beer, Acne Scars, 2018
Full-face deep peels should be carried out under full cardiopulmonary monitoring with intravenous hydration throughout the procedure. Intravenous sedation or regional blocks make the procedure pain free. A combination of oral sedative such as lorazepam, zolpiderm, alprazolam, or diazepam and analgesic, such as tramadol, can be also considered as optional. Before the peeling, meticulous degreasing of the skin is performed using oil-free acetone-soaked gauze sponges. This step is imperative to obtain even penetration of the solution into the skin.
Expression of Claudin-4 in Lung Ischemia-Reperfusion Injury in Experimental Lung Transplantation
Published in Journal of Investigative Surgery, 2022
Antonia Luna-Flores, J. Raúl Olmos-Zuñiga, Rogelio Jasso-Victoria, Miguel Gaxiola-Gaxiola, Teresa Aguirre-Pérez, Victor Ruiz, Rogelio García-Torrentera, Mariana Silva-Martínez, Edgar Zenteno, Gabriel Gutierrez-Ospina, Patricio Santillan-Doherty
A mid-sternotomy was performed, and the large vessels and the trachea were dissected. The pig was systemically heparinized (300 U/kg, IV) (Inhepar, Pisa, Guadalajara, Jalisco, México). A polypropylene (Prolene 4-0, Ethicon, Somerville, NJ, USA) purse-string suture was placed in the pulmonary trunk; then a 10 Fr cannula (Fem-Flex II Femoral Cannulae, Edwards Lifesciences, CA, USA) was inserted into the pulmonary trunk and secured with the purse-string suture. Afterwards the cannula was guided toward the left PA, and the lung was antegrade perfused with 20 mL/kg of a low potassium dextran solution (Perfadex, Vitrolife, Kungsbacka, Sweden) at a pressure of 20 cmH2O at 4 °C. Once the perfusion was concluded, to minimize pulmonary atelectasis, a volume recruitment maneuver was performed. The trachea was immediately clamped with two clamps to keep the lungs inflated, and the lung bloc was removed. Ex vivo, the artery and left bronchial were dissected and a left atrial cuff was done. Then, additional preservation solution was administered in a retrograde fashion through each of the left pulmonary veins to remove blood clots. Finally, the lung was wrapped in gauze sponges, placed in a plastic bag containing cold preservation solution and stored at 4 °C in a refrigerator for 50 min (group I) or 6 h (group II) [9,24,25].
A novel oral formulation of BIO 300 confers prophylactic radioprotection from acute radiation syndrome in mice
Published in International Journal of Radiation Biology, 2022
Vijay K. Singh, Oluseyi O. Fatanmi, Stephen Y. Wise, Alana Carpenter, Sara Nakamura-Peek, Artur A. Serebrenik, Michael D. Kaytor
All oral gavages via the po route were administered by securing the mouse at its scruff to extend the two front legs while immobilizing the head and neck. The gavage needle was then slowly inserted into the left side of the animal’s mouth and directed along the hard palate of the mouth to the back of the throat and guided to the abdominal area to deliver the bolus. The feeding needle was wiped and disinfected between dosing using a gauze sponge that was moistened with 70% ethanol (absolute alcohol diluted to 70%) to reduce the microorganisms and saliva on the feeding needle, and was then wiped with a purified water-soaked gauze sponge to reduce irritation to the mucosa before commencing dosing.
Management of full-thickness skin grafts
Published in Baylor University Medical Center Proceedings, 2021
Mitchell Davis, Daniel Baird, Dane Hill, Heather Layher, Russell Akin
Several methods of securing FTSG have been discussed in the literature. Most methods involve the use of a suture (Figure 1a) or staples to fasten various materials on top of the graft, thus protecting and securing the graft. Examples of materials used include gauze, sponges, buttons, and even sections of tongue depressors (Figure 1b, 1c).