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Electrophysiological Recording of a Gain-of-Function Polycystin-2 Channel with a Two-Electrode Voltage Clamp
Published in Jinghua Hu, Yong Yu, Polycystic Kidney Disease, 2019
Courtney Ng, Zhifei Wang, Bin Li, Yong Yu
Tricaine is kept at 4°C. Warm up the bottle to room temperature before opening the lid. Prepare a fresh 0.1% tricaine solution (1 g tricaine in 1 L deionized water), with the pH adjusted to 7.0 using NaHCO3.
Cinnamon attenuates adiposity and affects the expression of metabolic genes in Diet-Induced obesity model of zebrafish
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2019
Navrinder Kaur, Heerak Chugh, Vartika Tomar, Meena Kishore Sakharkar, Sujata K. Dass, Ramesh Chandra
Both before the start (week 0) and at end (week 4) of feeding experiments, body weight and length of anesthetized zebrafish were measured. In order to take measurements, fishes were anesthetized using buffered tricaine. Tricaine was prepared at 0.02% concentration in facility water and fish were transferred to a beaker containing the mixture. They were then monitored for stage III of anesthesia in which there was loss of equilibrium, loss of operculum movements and loss of reactivity. This stage was usually reached in a minute, after which measurements could be taken. Body weight in grams was measured by subtracting the weight of a beaker of facility water with the fish from the weight of the beaker of facility water only; using the Zebrafish Facility scale. Weight was determined every week during the experiment. Fish were allowed to recover from the anesthesia. Length in cm was measured from the anterior-most point of the mouth, to the most posterior-most region of the caudal peduncle using a ruler after body weight was taken. Length of each group was measured at week 0 and week 4 time-points.
MicroRNA-126 is a regulator of platelet-supported thrombin generation
Published in Platelets, 2020
Veronika Zapilko, Richard J. Fish, Alix Garcia, Jean-Luc Reny, Sylvie Dunoyer-Geindre, Thomas Lecompte, Marguerite Neerman-Arbez, Pierre Fontana
Aspirin (1 mM) was delivered into 5 day-old zebrafish larvae by direct soaking for 1 h at 27°C in the dark. Argatroban (Argatra®, Mitsubishi Tanabe Pharma, Düsseldorf, Germany), dissolved in ethanol (1 mg/ml), was administered by pericardial injection using a glass needle. The injection was performed under anesthesia with tricaine (164 mg/L), 20 min before the laser injury. As control, fish were injected with ethanol alone. To identify a possible effect of both drugs on thrombus formation, we analyzed laser-induced thrombocyte aggregation and evaluated occlusive thrombus formation as described above.
Dose and time response study to develop retinal degenerative model of zebrafish with lead acetate
Published in Cutaneous and Ocular Toxicology, 2022
Zebrafish was euthanized using 0.04% tricaine methanesulphonate. Eyes were enucleated and fixed overnight in 10% phosphate buffer formalin. The tissue processing was done following the method of Pearse19. Then tissue was embedded in paraffin wax and cut into 5 µm thick sections for histological analysis. The tissue was examined under light microscope (Leica DC 100, PC 1 Interface Digital Camera) at Department of Zoology, Panjab University, Chandigarh. The thickness of the outer nuclear layer, the inner nuclear layer and the photoreceptor damage were measured using Image J software.