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Predominantly Mature Interstitial Fibrosis
Published in Philip T. Cagle, Timothy C. Allen, Mary Beth Beasley, Diagnostic Pulmonary Pathology, 2008
Thomas Sporn, Victor L. Roggli
The identification of fibrosis in biopsy specimens may be considerably hindered by poor expansion of the alveoli as an artifact of processing in the surgical pathology laboratory. Airless and collapsed lung parenchyma that is otherwise normal may be misinterpreted as showing mature fibrosis. Accordingly, the interpretation of patterns of fibrosis within the lungs is best facilitated by proper inflation and fixation of the specimen. Although these procedures are most readily accomplished on whole lung or lobectomy specimens, techniques for inflation fixation of open or thoracoscopic lung biopsy specimens have been described and are recommended by the authors (1). Examination of sections using Masson’s trichrome stain may be useful in problematic cases. Transbronchial lung biopsies generally provide insufficient information to permit a secure diagnosis in cases typified by the pattern of mature fibrosis in the lung (2). Generous sampling by means of surgical lung biopsy is generally preferred and is often a requisite for an accurate diagnosis.
Morphological, biochemical, and histopathological effects of levetiracetam on pregnant albino rats and their offspring
Published in Ultrastructural Pathology, 2023
Safaa M. H. Abdelaziz, Ranya Mohammed Abdelgalil, Shaimaa R. Abdelmohsen
For light microscopy, livers were submerged in 10% saline for 3 days, at which point they were dried in increasing ethanol of 70%, 90%, and 100% and cleared with benzene. The specimens were impregnated in paraffin wax. The paraffin squares were cut by a rotatory microtome into serial transverse segment at (4 μm) thickness. Each five progressive transverse paraffin sections were mounted on an albumenized glass slide. The successive slides gotten from each example were stained with hematoxylin and eosin for demonstration of the common hepatic architecture.27 The collagen fibers were demonstrated using Masson’s trichrome stain.28 For transmission electron microscopy, little pieces of the livers (five specimens from five adult rats of IIB subgroup and 15 specimens from their offspring) were settled by immersed in 5% glutaraldehyde in 0.1 M sodium cacodylate buffer at pH 7.3. The specimens were at that point post-fixed with 1% osmium tetroxide within the same sodium cacodylate buffer. The specimens were at that point got dried out, cleared, and embedded in epoxy resin.29
Ameliorating effect of leukotriene receptor antagonist in multi-organ toxicity induced in rat offspring, a possible role for epidermal growth factor
Published in Immunopharmacology and Immunotoxicology, 2021
Walaa Yehia Abdelzaher, Remon Roshdy Rofaeil, Sara Mohammed Naguib Abdel-Hafez, Medhat Atta, Mohamed Ahmed Bahaa El-deen, Dalia Mohamed Ali
Opening a gate for certification of the previous biochemical study was done by histological studies. In AgNPs group, reduction of cortical region with distorted glomeruli and dilated tubules were noticed, which might be a compensatory mechanism after loss of renal excretory function of nephrons by tubular degeneration. All these findings are in accordance with previous studies [22,38,39]. Melanomacrophages were frequently noticed among the sections in group received AgNPs in renal and bone tissue. These cells are structurally similar to the mammalian germinal center, leading to the hypothesis that they played a critical role in the humoral adaptive immune response [40]. The bone tissue showed disorganized epiphyseal cartilage, which is in line with Qin and coworkers who found that release of silver ions and photocatalytic properties that facilitate oxidative stress thus led to tissue damage [39]. Masson’s trichrome stain was used to detect fibrosis [41]. Sections from the group received AgNPs showed obvious deposition of collagen between the renal tubules and bone tissue. All the previously mentioned histopathological changes in bone and kidney tissue occurred with AgNPs were ameliorated by MONT. Furthermore, anti-insulin immunoreactivity in bone marrow cells was reduced in AgNPs group and increased with MONT co-administration. These results are consistent with previous reports who suggested generation of insulin producing cells from bone marrow mesenchymal stem cells with undeniable role in cartilage growth and bone health [42–45].
Oral administration of green tea Epigallocatechin-3-gallate reduces oxidative stress and enhances restoration of cardiac function in diabetic rats receiving autologous transplantation of adipose-derived stem cells
Published in Archives of Physiology and Biochemistry, 2021
Tung-Sheng Chen, Show-Yih Liou, Hsin-Hung Lin, Meng-Yu Hung, Chien-Chung Lin, Yueh-Min Lin, Kuan-Ho Lin, V. Vijaya Padma, Chun-Hsu Yao, Wei-Wen Kuo, Chih-Yang Huang
Figure 2 shows HE stain (Figure 2(A)) and Masson’s trichrome stain (Figure 2(B)) for experimental rats. In Figure 2(A), compared to sham (round shaped ventricles), we can observe that the heart morphology is significantly changed in DM group (oval shaped ventricles). The heart morphology for both of DM + ADSC and DM + ADSC + E groups is restored when compared to DM group, especially in the DM + ADSC + E group. Similar result can be found in the thickness of ventricular septum for heart tissues. The left ventricular septum (black arrows) in DM heart is thinner than sham group. By contrast, thickness of heart tissues for DM + ADSC and DM + ADSC + E groups is restored when compared to DM group, especially in the DM + ADSC + E group. Figure 2(B) shows the collagen deposition in the heart tissues. The deposition of collagen is capable of visualising by Masson’s trichrome stain. Compared to sham group, the deposition of collagen (blue colour indicated by black arrows) in the heart tissue is more significant in DM group. On the other hand, the collagen deposition in both of DM + ADSC and DM + ADSC + E groups is insignificant when compared to DM group.