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A Potential Role for Immunological Mechanisms in Halothane Hepatotoxicity
Published in Robert G. Meeks, Steadman D. Harrison, Richard J. Bull, Hepatotoxicology, 2020
Andrea K. Hubbard, Timothy P. Roth, A. Jay Gandolfi
Using trifluoroacetylated rabbit serum albumin (TFA-RSA) as the detecting antigen, the TFA moiety was found to be part of the immunogen responsible for generating a halothane-induced antibody response in rabbits (Callis et al., 1987) (Figure 2) or guinea pigs (Siadat-Pajouh et al., 1987) receiving multiple halothane exposures. Indirect evidence then suggests that a reactive metabolic intermediate of halothane could alter a hepatocyte protein to produce an immunogen composed in part of the intermediate. The subsequent anti-TFA antibody could potentially damage hepatocytes incorporating this TFA-altered liver protein. Although proof for this theory is still under investigation, it is somewhat contradicted by the fact that the presence of high levels of specific anti-TFA antibody in animals prior to halothane exposure did not exacerbate or alter the transient liver damage induced by administration of this anesthetic (Mathieu et al., 1975b; Callis et al., 1987).
Microsurgery in the Portal Area of the Rat
Published in Waldemar L. Olszewski, CRC Handbook of Microsurgery, 2019
Changes in liver protein content varied. After about 6 weeks, adenomas were discovered in long-term surviving animals — however, not in such numbers as suggested in earlier literature. Stomach ulcers were also found in few cases only. After portacaval shunt operations male rats demonstrated testicular atrophy.
Biochemical Effects in Animals
Published in Stephen P. Coburn, The Chemistry and Metabolism of 4′-Deoxypyridoxine, 2018
Rosen et al.424 reported that deoxypyridoxine (100 mg/kg) injected into B6-deficient rats 4 hr prior to sacrifice almost doubled the liver uptake of an unspecified mixture of 20 labeled amino acids injected 2 hr prior to sacrifice compared with B6-deficient animals not receiving deoxypyridoxine. B6 deficiency alone produced about 50% increase in the radioactivity of liver protein compared with normal animals. Deoxypyridoxine caused no further change. B6 deficiency caused a slight increase in the radioactivity of thymus protein. Injection of deoxypyridoxine had no further effect on the radioactivity of amino acids on proteins in the thymus.
Open hepatic artery flow with portal vein clamping protects against bile duct injury compared to pringles maneuver
Published in Scandinavian Journal of Gastroenterology, 2023
Siliang Zhang, Pingli Cao, Pinduan Bi, Fu Yang, Ming Wu, Ding Luo, Bin Yang
Total liver protein from 100 mg of liver tissue was extracted and the protein concentration was determined by the BCA method. Proteins were separated in 10% SDS-PAGE and then transferred to polyvinylidene difluoride membranes. The membranes were blocked in Blocking Buffer (Beyotime, Shanghai, China) for 1 h at room temperature and then incubated with primary antibodies including rabbit monoclonal anti-ZO-1 (1:1000, ab221547, Abcam), rabbit polyclonal anti-claudin-1 (1:1000, 13050, Proteintech, China), and anti-claudin-3 (1:1000, 16456, Proteintech, China). Internal control used the β-actin (1:2000, Beyotime, Shanghai, China). All experiments were performed in triplicate.
Topo II inhibition and DNA intercalation by new phthalazine-based derivatives as potent anticancer agents: design, synthesis, anti-proliferative, docking, and in vivo studies
Published in Journal of Enzyme Inhibition and Medicinal Chemistry, 2022
Mohamed M. Khalifa, Ahmed A. Al-Karmalawy, Eslam B. Elkaeed, Mohamed S. Nafie, Mohamed A. Tantawy, Ibrahim H. Eissa, Hazem A. Mahdy
Liver enzymes ALT and AST were significantly increased to 63.4, 64.67 (U/L), respectively, following tumour inoculation as shown in Table 3, compared with normal mice at 45.14 and 53.67 (U/L) because of hepatocellular damage. While liver protein and albumin were decreased to 6.13 and 2.97 (g/dL). Treatment with compound 9d substantially reduced liver enzymes to 42.9, 55.6 U/L, respectively, and increased liver protein and albumin to 8.04 and 6.25 (g/dL), indicating a remarkable amelioration in the hepatocellular functions.
Effects of estrogen deficiency on liver function and uterine development: assessments of Medicago sativa's activities as estrogenic, anti-lipidemic, and antioxidant agents using an ovariectomized mouse model
Published in Archives of Physiology and Biochemistry, 2021
Hajer Jdidi, Fatma Ghorbel Kouba, Nissaf Aoiadni, Raed Abdennabi, Mouna Turki, Fatma Makni-Ayadi, Abdelfattah El Feki
After 21 and 60 days, the protein level in the uterus was significantly decreased respectively by 31 and 49% in OVX mice as compared with control groups. Liver protein level was also decreased by 13% in OVX mice after 8 weeks when compared with controls. Seven β-estradiol or Medicago sativa treatments restored uterus and liver protein levels without reaching control values (Table 8).