China
Africa
Explore chapters and articles related to this topic
Fluorescence Lifetime: Techniques, Analysis, and Applications in the Life Sciences
Published in Margarida M. Barroso, Xavier Intes, In Vivo, 2020
Jenu Varghese Chacko, Md Abdul Kader Sagar, Kevin W. Eliceiri
FLIM serves as one of the powerful quantitative imaging techniques to probe diverse biological interactions including protein binding, lipid binding, DNA binding, oxygen concentration through quenching kinetics, ion concentration, pH values, and aggregation through changes in knr and kr, and transient local environments relative to a fluorophore. Current FLIM implementations are broadly classified into a flowchart (Figure 9.5). This list is definitely not exclusive, but rather a representation of different schemes.
Advanced Light Microscopy Techniques
Published in Jay L. Nadeau, Introduction to Experimental Biophysics, 2017
FLIM is a relatively new technique, so the extent of changes to lifetimes with environment remains to be explored for a wide range of dyes, FPs, and intrinsic fluorophores. The degree to which lifetime imaging may be useful for ultraresolution techniques also remains largely unknown, as most photoactivated dyes have not been characterized by lifetime analysis. It is only recently that FLIM has become available to a large number of academic researchers, so the number of possible applications is only beginning to be appreciated.
Spectro-Temporal Autofluorescence Contrast–Based Imaging for Brain Tumor Margin Detection and Biobanking
Published in Yu Chen, Babak Kateb, Neurophotonics and Brain Mapping, 2017
Asael Papour, Zach Taylor, Linda Liau, William H. Yong, Oscar Stafsudd, Warren Grundfest
Fluorescence lifetime imaging microscopy (FLIM) is a research technique that extracts the autofluorescence lifetime from the fluorescence intensity decay at each point on the tissue sample and delineates tissue constituents based on variations in decay time. This is accomplished by exciting the sample with a very short (picosecond) pulse of a UV laser and detecting the autofluorescence decay coefficient at a range of wavelengths (Marcu et al. 2004; Lakowicz 2006; Sun et al. 2010).
Measuring time with high precision in particle physics
Published in Radiation Effects and Defects in Solids, 2022
B. Kaynak, S. Ozkorucuklu, A. Penzo
High-precision time information is recognized as a crucial enabling factor for many large-scale research fields. Some typical examples in different fields are: Time-of-Flight (TOF) in High-Energy Physics (HEP) for Particle Identification (PID) (1,2);Time-Resolved Spectroscopy/Fluorescence Lifetime Imaging Microscopy (3,4);High-Resolution Pulsed Laser Ranging for long-distance measurements (5,6);Imaging by Time-of-Flight Positron Emission Tomography (TOF-PET) (7,8).These different applications, therefore, share a common need for highly time-resolved detection systems to improve event timing and increase detector throughput. Modern vacuum-based detector technologies reach resolutions below 10 ps (Root Mean Square, RMS) (9); recent progress in solid-state photo-detectors reduces time resolutions from hundreds to tens of picoseconds (RMS) (10).