China 
Africa 
Explore chapters and articles related to this topic
The Proofreading Step
Published in Marialuisa Aliotta, Mastering Academic Writing in the Sciences, 2018
Some people tend to identify proofreading with spellchecking but this is only one aspect of it. The key purpose of proofreading your work is to detect and correct not just typographical errors but also any mistakes in grammar, punctuation, and references. It also implies checking that your text is formatted consistently and adheres to specific editorial conventions, as prescribed by different journals and universities guidelines.
Optimized expression of large fragment DNA polymerase I from Geobacillus stearothermophilus in Escherichia coli expression system
Published in Preparative Biochemistry & Biotechnology, 2023
Eva Agustriana, Isa Nuryana, Fina Amreta Laksmi, Kartika Sari Dewi, Hans Wijaya, Nanik Rahmani, Danu Risqi Yudiargo, Astadewi Ismadara, Moch Irfan Hadi, Awan Purnawan, Apridah Cameliawati Djohan
In this study, the Bst DNA polymerase gene was synthesized and harbored in the plasmid pD451-SR. The gene was designed following the original sequence of the large-fragment DNA polymerase I gene from Geobacillus stearothermophilus with codon optimization. According to patent no CN106399299A[13], the sequence of the gene has a 1758 bp-nucleotide and encodes a 586 aa-protein with a predicted molecular weight of 67.8 kDa. The enzyme can be functional and thermostable in the temperature range between 25 °C and 75 °C with the optimum temperature at 65 °C[19]. A previous study has reported the full-length of the Bst DNA polymerase I gene consisted of 2648 bp with a predicted molecular weight of 98.6 kDa[20]. Belonging to DNA polymerase type I, Bst DNA polymerase with the complete sequence comprises three kinds of activity: (I) 5′→3′ exonuclease activity, (II) 5′→3′ DNA polymerase activity, and (III) 3′→5′ exonuclease activity[21]. Domains II and III are located in the C-terminus of Bst DNA polymerase and indicated as the large fragment which has no 5′→3′ proofreading exonuclease activity. The sequence gene of the large fragment excludes a 5′ terminal containing up to 876 bp in length, hence a predicted molecular weight of protein translated is decreased to be around 67 kDa[10,22,23]. Bst DNA polymerase used in this study was indicated to belong to the large fragment for sure.
Cost-effective, high-yield production of Pyrobaculum calidifontis DNA polymerase for PCR application
Published in Preparative Biochemistry & Biotechnology, 2023
Kashif Maseh, Syed Farhat Ali, Shazeel Ahmad, Naeem Rashid
3′-5′ Exonuclease activity confers proofreading ability to DNA polymerase. 3′-5′ exonuclease activity of Pca-Pol was analyzed by degradation of a 5′ biotin labeled 40-mer ‘primer’ annealed to an 80-mer template. Pca-Pol showed 3′-5′ exonuclease activity by degrading the primer (Figure 4).