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Halophilic Microbiome
Published in Ajar Nath Yadav, Ali Asghar Rastegari, Neelam Yadav, Microbiomes of Extreme Environments, 2021
Mrugesh Dhirajlal Khunt, Rajesh Ramdas Waghunde, Chandrashekhar Uttamrao Shinde, Dipak Maganlal Pathak
Betaine is another compatible solute, synthesized by halophilic microbes in response of abiotic stresses, is an osmoprotectant and methyl group donor that inhibits nuclear factor-κB activity and NLRP3 inflammasome activation, regulates energy metabolism and mitigates endoplasmic reticulum stress, apoptosis, and in turn also has the beneficial effect of human diseases such as obesity, diabetes, cancer, and Alzheimer’s disease (Zhao et al. 2018). Betaine has a positive effect on internal human organs as it improves vascular system and enhances its performance, while also being an important nutrient for the prevention of chronic diseases (Craig 2004). It also finds application in PCR amplification to increase yield and specificity of GC rich DNA templates (Roberts 2005). Actinoployspora sp., Halorhodopira halochloris, Thioalkalivibrio versutus have been characterized for their betaine production (Shivanand and Mugeraya 2011).
Big Data and Transcriptomics
Published in Shampa Sen, Leonid Datta, Sayak Mitra, Machine Learning and IoT, 2018
Sudharsana Sundarrajan, Sajitha Lulu, Mohanapriya Arumugam
The raw sequence-based metrics checks the experiments at low-level as prior sequence alignments are not required. The raw sequence quality is assessed based on the Phred quality score (Q). Phred score measures the base-calling reliability from Sanger sequencing chromatograms. It is defined as Q = −10 × log10(P), where P is the probability of erroneous base calling. GC content gives the percentage of either guanine or cytosine bases in a DNA sequence. It is a simple way to measure the nucleotide composition. Read duplication is often determined by read length, transcript abundance, PCR amplification, and sequence depth.
Identification and characterization of candidates involved in production of OMEGAs in microalgae: a gene mining and phylogenomic approach
Published in Preparative Biochemistry and Biotechnology, 2018
Vikas U. Kapase, Asha A. Nesamma, Pannaga P. Jutur
The GC content has significant role in gene and gene regulation as well as in determining the physical properties of DNA molecules. Among the most important features of the GC content is its correlation with higher thermal stability compared to AT base pair.[48] Oliver et al. reported that the average reading frame length in GC-rich sequences is expected to be larger than in the GC-poor ones.[49] The GC content is predicted to significantly affect genome functioning, species ecology and positively correlated with the physical size of the genome.[50] Our analysis predicts that all these OMEGA gene-encoding proteins have more than 60% GC content, while among all acetyl-CoA acyltransferase shows highest GC content (Figure 3).