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Control of workplace health hazards
Published in Sue Reed, Dino Pisaniello, Geza Benke, Kerrie Burton, Principles of Occupational Health & Hygiene, 2020
Training programs should be formalised and administered long past the induction period and throughout the length of employment. Training should always incorporate the practical aspects of a job and include some form of competency assessment. If workers potentially exposed to a hazard are made fully aware of the consequences of over-exposure, and the routes and mechanisms of exposure, they are more likely to identify other exposure situations and act to reduce exposures in new situations. In this regard, it is useful in a training setting to visually represent exposure. For example, fluorescein dye (a dye used by plumbers to trace drains) can be added to aqueous solutions to simulate toxic liquids. In order to show the degree of containment, or the spread of contamination, the traces of fluorescein can be made visible by using a UV lamp. Smoke generators can be used to test ventilation systems and can show the potential movement of contaminants within the workplace. Intense lighting can also be used to show dust generation, as seen in Figure 4.4.
Control strategies for workplace health hazards
Published in Sue Reed, Dino Pisaniello, Geza Benke, Principles of Occupational Health & Hygiene, 2020
Training programs should be formalised and administered long past the induction period and throughout the length of employment. Training should always incorporate the practical aspects of a job and include some form of competency assessment. If workers potentially exposed to a hazard are made fully aware of the consequences of over-exposure, and the routes and mechanisms of exposure, they are more likely to identify other exposure situations and act to reduce exposures in new situations. In this regard, it is useful in a training setting to visually represent exposure. For example, fluorescein dye (used by plumbers to trace drains) can be added to aqueous solutions to simulate toxic liquids. In order to show the degree of containment, or the spread of contamination, the traces of fluorescein can be made visible by using a UV lamp. Smoke generators can be used to test ventilation systems and can show the potential movement of contaminants within the workplace. Intense lighting can also be used to show dust generation, as seen in Figure 4.7.
Measurement and Analysis of Flow
Published in James L. Martin, Steven C. McCutcheon, Robert W. Schottman, Hydrodynamics and Transport for Water Quality Modeling, 2018
James L. Martin, Steven C. McCutcheon, Robert W. Schottman
Many dyes become unstable as pH varies due to ionization or structural changes. For dyes that ionize, adjustments can allow correction for pH reversible effects. There is evidence that the fluorescence of fluorescein dye is susceptible to reversible structural changes as the pH changes. (Smart and Laidlaw 1977).
The effect of freeze-drying and storage on lysozyme activity, lactoferrin content, superoxide dismutase activity, total antioxidant capacity and fatty acid profile of freeze-dried human milk
Published in Drying Technology, 2022
Dorota Martysiak-Żurowska, Patrycja Rożek, Małgorzata Puta
The TAC of milk was determined in the oxygen radical absorbance capacity assay with fluorescein (ORAC-FL) based on the procedure described by Saenz, Elisia, Innis, Friel & Kitts.[24] In the ORAC method, peroxyl radicals are generated during the thermal decomposition of 2,2′-azobis(2-aminopropane) dihydrochloride (AAPH) dissolved in a phosphate buffer (pH= 7.4). The free radical damage to fluorescein (FL) leads to a decrease in fluorescent intensity. The presence of antioxidants inhibits free radical damage to the fluorescent compound. Reduction the degradation rate of fluorescein over time is proportional to the antioxidant content of the solution. Milk samples were diluted 1:199 with a phosphate buffer. All measurements were performed with a microplate reader (Synergy HT, BIOKOM). The value of TAC was expressed in µM of Trolox (TE) per mL of milk.
Synthesis and characterization of novel fluorescent reactive dyes for dyeing of cotton fabrics
Published in The Journal of The Textile Institute, 2022
Maral Pishgar, Kamaladin Gharanjig, Mohammad Esmail Yazdanshenas, Khosro Farizadeh, AboSaeed Rashidi
Fluorescein as a xanthene dye has excellent photophysical properties and high intensity of emission wavelength. So, it was remarkable and desirable. Von Bayer synthesized fluorescein in 1871 by Friedel-Crafts reaction of anhydride phthalic, resorcinol, zinc chloride as a catalyst in high temperature (170–180 °C) under HCl concentrated (Brandt & Keston, 1965) for the first time (Grimm & Lavis, 2011; Matsui et al., 2017).
A batch and fixed bed column study for fluorescein removal using chitosan modified by epichlorohydrin
Published in Journal of Environmental Science and Health, Part A, 2018
P. M. O. Silva, J. E. Francisco, J. C. M. Cajé, R. J. Cassella, W. F. Pacheco
Fluorescein (FSC) is a yellow anionic dye from the xanthenes series, synthesized by Bayer in 1871. The xanthene class had initial application in the dyeing of clothes, however, was soon abandoned due to loss of coloration when the stained fibre was exposed to light (photobleaching).