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Arsenals of Pharmacotherapeutically Active Proteins and Peptides: Old Wine in a New Bottle
Published in Debarshi Kar Mahapatra, Swati Gokul Talele, Tatiana G. Volova, A. K. Haghi, Biologically Active Natural Products, 2020
Reaction with Dansyl Chloride: Amino group of amino acids react with dansyl chloride (1-dimethylaminonaphthalene-5-sulfonyl chloride) to form dansyl derivatives. These dansyl derivatives resist acid hydrolysis and therefore can be used for establish the N-terminal amino group or ε-amino group of peptides and proteins [106]. Dansyl derivatives are highly fluorescent and can be identified by performing thin layer chromatography on sheets of polyamide. This reaction was originally developed for detection amino acids in peptides but can also be used for proteins [107].
Amino Acids, Peptides, and Proteins
Published in Michael B. Smith, A Q&A Approach to Organic Chemistry, 2020
Dansyl chloride is 5-dimethylamino-1-naphthalenesulfonyl chloride. The N-terminal amino acid of a peptide (A) will displace the fluorine to form an N-aryl derivative, sulfonamide B. Hydrolysis leads to cleavage of the N-terminal amino acid residue from the peptide as a dansyl amino acid (C), (5-(dimethylamino)naphthalen-1-yl)sulfonyl)glycine. This compound is fluorescent and easily detected in the presence of the other amino acids liberated in the hydrolysis step.
Determination of mercury (II) in water samples by fluorescence using a dansyl chloride immobilized glass slide
Published in Instrumentation Science & Technology, 2020
Imran Muhammad, Turghun Muhammad, Amina Hoji, Xiaoxia Yang, Aziguli Yigaimu
Salinized glass slides were treated with freshly prepared dansyl chloride solution 0.0269 g (0.1 mmol) in 6.0 mL anhydrous acetonitrile in the presence of potassium carbonate (0.1 g) as a basic catalyst for 24 hours at room temperature with gentle shaking. The prepared sensor slide was washed with methanol to remove all of the unbounded dansyl chloride. After confirmation of complete removal of unbounded dansyl chloride with fluorescence using a spectrofluorometer, the slides were dried at room temperature. The structure of dansyl chloride and the modification procedure are shown in Figure 1.